Results 21 to 30 of about 14,469 (162)
Veterinary Dermatology, Volume 36, Issue 3, Page 291-301, June 2025.Background – Canine interdigital furunculosis (CIF) is a complex, relapsing inflammatory condition, typically complicated by deep bacterial infections requiring prolonged systemic antibiotics. Hypothesis/Objectives – This split‐body study, where dogs acted as their own control, evaluated whether the adjunctive use of fluorescent light energy (FLE ...
A. Lange +12 more
wiley +1 more source
Ultrasound in Obstetrics &Gynecology, Volume 65, Issue 4, Page 470-477, April 2025.ABSTRACT Objective To evaluate the presence and levels of cytomegalovirus (CMV) cell‐free DNA (cfDNA) fragments in women pregnant with a fetus with symptomatic congenital CMV (cCMV). Methods The study comprised nine women whose fetuses were diagnosed with cCMV between June 2019 and July 2024 at 20 + 4 to 34 + 1 weeks' gestation (n = 8) or neonatally (n
B. H. W. Faas +9 more
wiley +1 more source
Veterinary Dermatology, Volume 35, Issue 5, Page 500-507, October 2024.Abstract Background Canine atopic dermatitis (cAD) is a hereditary, generally pruritic and predominantly T‐cell‐driven inflammatory skin disease, involving an interplay between skin barrier abnormalities, allergen sensitisation and microbial dysbiosis.
L. Widorn +2 more
wiley +1 more source
Fetal and neonatal neuroimaging in twin–twin transfusion syndrome
Ultrasound in Obstetrics &Gynecology, Volume 63, Issue 6, Page 746-757, June 2024.ABSTRACT Objectives To describe the types of brain injury and subsequent neurodevelopmental outcome in fetuses and neonates from pregnancies with twin–twin transfusion syndrome (TTTS). Additionally, to determine risk factors for brain injury and to review the use of neuroimaging modalities in these cases.
M. S. Spruijt +8 more
wiley +1 more source
pEgr-hPTEN体外稳定转染对人脑胶质瘤SHG-44细胞周期及增殖的影响
Zhongguo shiyan zhenduanxue, 2004 目的 探讨外源野生型PTEN基因对人脑胶质瘤SHG 4 4细胞周期及增殖的影响 ,阐明PTEN基因抑制肿瘤细胞增殖的机制。方法 脂质体包裹重组质粒pEgr hPTEN体外转染内源性PTEN基因突变的SHG 4 4胶质瘤细胞 ;G4 18筛选稳定表达细胞株并扩增培养 ;采用细胞计数法检测肿瘤细胞增殖速度 ;流式细胞术检测肿瘤细胞周期的变化。结果 经 10到 2 0dG4 18筛选得到稳定表达细胞株 ;重组质粒pEgr hPTEN稳定转染可明显抑制SHG 4 4细胞的体外增殖 ,细胞周期明显改变 ...
田梅, 朴春姬, 李修义, 杨巍
doaj
The phosphorylation of BAG3 by CDK5 and its roles in astrocytes [PDF]
, 2016 细胞周期素依赖性蛋白激酶5（Cyclin-DependentKinase5，CDK5)是细胞周期蛋白依赖性丝氨酸/苏氨酸蛋白激酶家族中的一个成员，在神经系统的发育中发挥了至关重要的作用。CDK5的调节亚基P35、P39是神经系统特异性表达的蛋白，因此赋予了CDK5在神经系统中特异的激酶活性，使CDK5在调控神经递质传递，神经元凋亡等方面发挥了重要作用。对于CDK5的研究一直集中在对其新底物的发现和鉴定上，至今为止有40余个CDK5的底物被鉴定出来。作为脯氨酸依赖性蛋白激酶，CDK5可以特异磷酸化包含有 ...
张冠云
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Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】研究转 HBx 基因肝癌细胞增殖与拮抗凋亡特性,探讨肝癌恶性表型的分子基础。【方法】以携 HBx 基因 重组逆转录病毒感染 QGY7701肝癌细胞, G418 筛选,PCR 与 RT-PCR 鉴定; 流式细胞仪( FCM)检测细胞增殖周期; 用阿霉素 诱导细胞凋亡,FCM 定量检测。【结果】QGY7701 肝癌细胞经 HBx 基因转染后,G418 筛选 4～ 6 周获阳性克隆株 QGY/HBx, PCR 与 RT -PCR 分别证实细胞有 HBx 基因整合与 HBx mRNA 表达 ...
闵 军, 刘彦文, 余新炳
doaj
The expression and function of Brg1 in ocular surface tissues under normal and disease situation [PDF]
, 2015 背景：Brahma相关基因1（Brahma-relatedgene1，Brg1）是染色质重塑复合物SWI/SNF的ATP酶亚基之一，广泛存在于真核细胞中。以往的研究表明，Brg1结合复合物参与不同类型细胞的细胞周期控制、凋亡和细胞分化。到目前为止，没有关于眼表上皮细胞Brg1表达以及功能的报道。 方法：对小鼠、兔以及人眼表组织的Brg1表达和定位进行免疫荧光染色和蛋白质印迹检测。人角膜上皮细胞株（HCE），小鼠角膜上皮细胞系（TKE2），小鼠原代培养的角膜上皮细胞（mCEC）分别在DMEM/F12+10％
刘婷婷
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pEgr-hp53体外稳定转染联合辐射对人肺腺癌A549细胞周期进程及增殖的影响
Zhongguo shiyan zhenduanxue, 2008 目的探讨pEgr-hp53重组质粒体外稳定转染联合辐射对人肺腺癌A549细胞周期进程及增殖的影响,阐明其抑制肿瘤细胞增殖的机制。方法脂质体包裹重组质粒pEgr-hp53体外转染A549细胞,G418筛选稳定表达细胞株并扩增培养,采用细胞计数法检测肿瘤细胞增殖速度,流式细胞术检测肿瘤细胞周期的变化。结果经pEgr-hp53体外转染A549细胞10到20d,G418筛选得到稳定表达的细胞株,联合X射线照射可明显抑制A549细胞的体外增殖,细胞周期进程明显改变,G1期和G2期发生阻滞。结论提示pEgr ...
董丽华, 刘锋, 李艳博, 龚守良
doaj
Cloning and function analysis of rice OsICK1 gene [PDF]
, 2006 细胞分化是植物生长和发育的重要过程。在真核生物中，细胞周期蛋白依赖性激酶（CDK）是细胞周期调控的中心组分，对细胞周期起着核心性的调控作用。细胞周期蛋白依赖性激酶（CDK）能够与细胞周期蛋白（cyclin）结合，形成Cyclin-CDK复合物，激活CDK的激酶活性，使底物蛋白质磷酸化，调控细胞周期的运行。不同种类的CDK能够与不同种类的周期蛋白结合，构成不同的CDK激酶，在细胞周期的不同时期表现出活性，对细胞周期的不同时期进行调节。细胞周期蛋白依赖性激酶抑制剂(ICK ...
何艺宾
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