【目的】对成人视网膜前体细胞进行体外分离、培养和鉴定。【方法】成人眼球 12 只,“机械分离联合酶消化法”分离出睫状上皮层的色素性细胞, 10 ng/mL 碱性成纤维细胞生长因子(bFGF) +10 ng/mL 表皮生长因子(EGF) 的无血清 DMEM/F12 培养液中培养, 并从三方面鉴定其神经干细胞特性: A. 免疫荧光染色检测神经干细胞特异性抗原神经巢蛋白 Nestin 的表达 ; B. 自我更新能力 : 原代神经球消化传代后 , 继续培养观察新神经球的形成; C. 多向分化潜能: 原代细胞培养
唐仕波, 林少芬
doaj
[Dihydroartemisinin inhibits the progression of oral squamous cell carcinoma]. [PDF]
Shan T +5 more
europepmc +1 more source
【目的】 探讨体外分离培养细针活检滑膜组织成纤维样滑膜细胞(FLS)的方法65377;【方法】 滑膜组织来自接受盲式细针滑膜活检术的活动期类风湿关节炎患者,分别采用消化酶培养法65380;组织块培养法和改良组织块培养法分离培养FLS65377;采用台盼蓝染色进行细胞计数及活性鉴定,并应用倒置相差显微镜65380;透射电镜65380;免疫细胞化学染色65380;流式细胞术对第3 ~ 4代FLS进行鉴定65377;【结果】 3种原代分离培养的方法均可成功培养出FLS65377;台盼蓝染色示FLS细胞计数约 ...
doaj
[<i>Huangqin Qingre Chubi</i> Capsule inhibits JAK/STAT-driven synovial angiogenesis in rheumatoid arthritis by suppressing the LncRNA EBLN3P/miR-369-3p/NFIX axis]. [PDF]
Sun M, Wang Y, Liu F.
europepmc +1 more source
[Advances in the construction and application of a novel liver organoid model for hepatitis B virus infection]. [PDF]
Li H, Xiang KH.
europepmc +1 more source
[Protective effect and mechanism of TSPAN9-mediated mitocytosis in interleukin-1β-induced rat chondrocyte senescence]. [PDF]
Chen Q, Da W, Luo N, Shen B.
europepmc +1 more source
[Effects of polyvinyl alcohol-boric acid-based functionalized hydrogels on HSFs and HaCaT cells]. [PDF]
Song W +13 more
europepmc +1 more source
[Effects of cold atmosphere plasma treatment on the biological behavior of human gingival fibroblasts]. [PDF]
Zheng M +7 more
europepmc +1 more source
[Selenocystine inhibits colon cancer cell growth by promoting reactive oxygen species generation to trigger oxidative damage]. [PDF]
Song Q +8 more
europepmc +1 more source
[A preliminary study on the role of platelet mitochondria in the proliferation and metabolism of multiple myeloma cells]. [PDF]
Zhang LY, Xiang YH, Li YY, Zhang J.
europepmc +1 more source

