Results 121 to 130 of about 9,595 (163)

肝星状细胞通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖

Zhongshan Daxue xuebao. Yixue kexue ban, 2016
【目的】研究肝星状细胞(HSC)是否通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖。【方法】流式细胞仪分选出CD3+ T细胞，用羧基荧光素双乙酸盐琥珀酰亚胺酯(CFSE)标记后与人肝星状细胞系LX2细胞共培养, PHA刺激72 h后,流式细胞仪检测T细胞增殖的百分比;为了研究肝星状细胞调控T细胞增殖的机制，将CD3+ T细胞与LX2细胞共培养3 d，流式细胞仪检测CD8+CD28-T淋巴细胞的百分比。进一步将CD3+ T细胞与LX2细胞共培养3 d，通过流式细胞仪分选出CD8+CD28 ...
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[Establishment of an Epstein-Barr virus infection model using human nasal organoids]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wei J, Zheng H, Zhao Y, Yu Y, Xu Y, Li G.   +5 more
europepmc   +1 more source

最优化细胞因子诱导杀伤细胞/自然杀伤细胞 少因子培养体系的探索

Zhongshan Daxue xuebao. Yixue kexue ban, 2009
【目的】 尝试使用IL-2、IL-7和IL-12的单因子双因子和三因子组合进行杀伤细胞/自然杀伤细胞(CIK/NK)细胞扩增培养,探讨最优化CIK/NK细胞少因子培养体系的建立方法】 采用Ficoll-Hypaque法分离脐血单个核细胞并分成6组,每组分别加入IL-2(80 ng/mL) IL-7(40 ng/mL)及IL-12(40 ng/mL)的单因子双因子和三因子组合(IL-7组;IL-12 组;IL-7+IL-12组;IL-2 + IL-7组;IL-2 + IL-12组;IL-2 + IL-7 +
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[Mefloquine HCl promotes DNA repair and alleviates radiation-induced lung epithelial cell injury]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang Y, Wen Q, Huang H, Zhou M, Wang J.
europepmc   +1 more source

[Functional characterization of double-negative T cells isolated from leukoreduction filter residues]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Xiang Z, Wu Y, Huang K, Wu F, Lin J, Sang L, Yang L.   +6 more
europepmc   +1 more source

人诱导多能干细胞向外周神经元分化的实验研究

Zhongshan Daxue xuebao. Yixue kexue ban, 2014
摘要： 【目的】 探讨人诱导多能干细胞（ｈｉＰＳ细胞）在体外向外周神经元分化的能力。【方法】人诱导多能干细胞在无饲养层培养条件下维持培养，然后在神经培养基中悬浮培养分化为神经球，通过把神经球贴壁在多聚赖氨酸和层粘连蛋白包被的培养板上，利用流式分选细胞方法获得神经嵴干细胞（ＮＣＳＣ），通过把神经嵴干细胞在外周神经诱导培养基中诱导分化，获得外周神经元，利用免疫荧光染色的方法检测神经嵴干细胞和外周神经元的标记物。【结果】贴壁的神经球出现特征性的神经花环（Ｎｅｕｒａｌ ｒｏｓｅｔｔｅｓ）结构 ...
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[cGAS-STING agonist cGAMP enhances natural killer cell-mediated cytotoxicity against gastric cancer cells]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wang Q, Chai Z, Deng Y, Zhang Z, Gong Y, Gao S, Feng P.   +6 more
europepmc   +1 more source

[Sulforaphane reduces reactive astrocyte-mediated neuron apoptosis <i>in vitro</i> by inhibiting the MAPK/NF-κB signaling pathway in Aβ42 oligomer-activated astrocytes]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang S, Huang T, Yang C, Chen J, Lü T, Zhang J.   +5 more
europepmc   +1 more source

[Expression and significance of the <i>FABP6</i> long transcript in clear cell renal cell carcinoma]. [PDF]

Beijing Da Xue Xue Bao Yi Xue Ban
Yin H, Wang Z, Shu F, Zhang Z, Liang H, Zhang S.   +5 more
europepmc   +1 more source

[Total Astragalus saponins reduce astrocyte swelling and AQP4 expression following oxygen-glucose deprivation and reoxygenation by inhibiting the p38 pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Yin Q, Lei Y, Wang X, Yang G, Tang Z.
europepmc   +1 more source