肝星状细胞通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖
Zhongshan Daxue xuebao. Yixue kexue ban, 2016 【目的】研究肝星状细胞(HSC)是否通过诱导产生CD8+CD28-T淋巴细胞抑制T细胞增殖。【方法】流式细胞仪分选出CD3+ T细胞，用羧基荧光素双乙酸盐琥珀酰亚胺酯(CFSE)标记后与人肝星状细胞系LX2细胞共培养, PHA刺激72 h后,流式细胞仪检测T细胞增殖的百分比;为了研究肝星状细胞调控T细胞增殖的机制，将CD3+ T细胞与LX2细胞共培养3 d，流式细胞仪检测CD8+CD28-T淋巴细胞的百分比。进一步将CD3+ T细胞与LX2细胞共培养3 d，通过流式细胞仪分选出CD8+CD28 ...
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[Angio-associated migratory cell protein is highly expressed in hepatocellular carcinoma and promotes tumor cell invasion and metastasis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLi C, Yin G, Cheng X, Jiang Y.
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[Indole-3-acetic acid alleviates <i>Cryptococcus neoformans</i>-induced pyroptosis in cerebral microvascular endothelial cells by regulating stress granule-mediated NLRP3 inflammasome activation]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoChen J +5 more
europepmc +1 more source
最优化细胞因子诱导杀伤细胞/自然杀伤细胞 少因子培养体系的探索
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 尝试使用IL-2、IL-7和IL-12的单因子双因子和三因子组合进行杀伤细胞/自然杀伤细胞(CIK/NK)细胞扩增培养,探讨最优化CIK/NK细胞少因子培养体系的建立方法】 采用Ficoll-Hypaque法分离脐血单个核细胞并分成6组,每组分别加入IL-2(80 ng/mL) IL-7(40 ng/mL)及IL-12(40 ng/mL)的单因子双因子和三因子组合(IL-7组;IL-12 组;IL-7+IL-12组;IL-2 + IL-7组;IL-2 + IL-12组;IL-2 + IL-7 +
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[Effect of dephosphorylation of tumor metastasis suppressor gene <i>LASS2</i> on vacuolar ATPase activity and invasiveness of prostate cancer]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanLiu Y +8 more
europepmc +1 more source
[The role of extracellular vesicles in early embryo development and their application in assisted reproductive technologies]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanWang H, Li X, Lan H, Tong X.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘要： 【目的】 探讨人诱导多能干细胞（ｈｉＰＳ细胞）在体外向外周神经元分化的能力。【方法】人诱导多能干细胞在无饲养层培养条件下维持培养，然后在神经培养基中悬浮培养分化为神经球，通过把神经球贴壁在多聚赖氨酸和层粘连蛋白包被的培养板上，利用流式分选细胞方法获得神经嵴干细胞（ＮＣＳＣ），通过把神经嵴干细胞在外周神经诱导培养基中诱导分化，获得外周神经元，利用免疫荧光染色的方法检测神经嵴干细胞和外周神经元的标记物。【结果】贴壁的神经球出现特征性的神经花环（Ｎｅｕｒａｌ ｒｏｓｅｔｔｅｓ）结构 ...
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[Effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiTang LJ +6 more
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[Preparation of polycaprolactone-polyethylene glycol-concentrated growth factor composite scaffolds and the effects on the biological properties of human periodontal ligament stem cells]. [PDF]
Hua Xi Kou Qiang Yi Xue Za ZhiGao L +5 more
europepmc +1 more source
[Exosomes from folic acid-treated subpatellar fat pad-derived mesenchymal stem cells promote M2 polarization of macrophages <i>in vitro</i>]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWang Z +9 more
europepmc +1 more source