Results 61 to 70 of about 1,600 (128)
Effect of neferine on diabetic nephropathy by regulating SDF-1/CXCR4 signal pathway [PDF]
Objective·To investigate the effect of neferine (Nef) on renal tissues of diabetic nephropathy (DN) rats and its related mechanism.Methods·DN model rats were constructed by feeding high-fat diet combined with intraperitoneal injection of streptozotocin ...
LIU Lei +4 more
core +1 more source
携带人可溶性TRAIL基因的重组腺病毒在肺癌细胞中的转染效率及表达特性研究
Zhongguo shiyan zhenduanxue, 2010 目的研究重组腺病毒感染肺腺癌细胞A549的转染效率及重组腺病毒介导的人可溶性肿瘤坏死因子相关诱导凋亡配体（hTRAIL95-281）基因的体外表达。方法用不同感染滴度（MOI）（0-10 000 VP/cell）的重组腺病毒Ad-CMV-EGFP转染A549细胞后48 h收集细胞,经流式细胞仪检测其转染效率,并在共聚焦荧光显微镜下观察绿色荧光蛋白表达;用MOI为5 000 VP/cell的Ad-CMV-EGFP感染肿瘤细胞后不同时间（24 h、48 h、72 h和96 h）收集细胞 ...
朴春姬, 田梅, 杨巍, 李修义
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2011 【目的】 研究海洛因对乳鼠心肌细胞钙活动的影响以及维拉帕米对海洛因作用下的乳鼠心肌细胞钙活动的影响作用【方法】 使用体外培养5 d的SD乳鼠心肌细胞,标记细胞内游离钙,应用激光共聚焦显微镜下检测细胞内荧光强度变化可指示细胞内游离Ca浓度([Ca]i)变化;并应用碘化丙啶进行活性细胞检测【结果】 0.01 mol/L海洛因可增加培养心肌细胞平均细胞内钙浓度和钙瞬变的幅度,对自发性收缩节律无明显影响;20 μmol/L维拉帕米可明显抑制海洛因诱导的钙超载,并可降低收缩节律,减少细胞死亡【结论 ...
doaj
浙江大学学报. 农业与生命科学版Benzo[a]pyrene (BaP), an injurant produced in high-temperature processing foods, has been reported to cause colon injury. This study sought to investigate whether habitual consumption of whole grain buckwheat (WGB) or oat (WGO) could prevent 50 mg/kg BaP-
贾颖宇(JIA Yingyu) +7 more
doaj +1 more source
Research Progress of ROS-Mitochondrial Pathway on Cardiovascular Protection of Tea Polyphenols [PDF]
Reactive oxygen species (ROS) imbalance and mitochondrial dysfunction played critical roles in the pathogenesis of cardiovascular diseases which are the leading cause of death worldwide.
Jinchan PI +5 more
core +1 more source
Significant fundamental translational research on breast cancer in China: progress and prospects [PDF]
Breast cancer is the most prevalent malignant tumor that poses a threat to women's health in China, with incidence and mortality rates persistently increasing. Given this critical situation, there is an urgent need to optimize therapeutic options through
LU Yufeng, WANG Han, XIE Yifan, JIANG Yizhou, SHAO Zhimin
core +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2012 【目的】 探讨Akt抑制剂124005对人鼻咽癌细胞CNE1放射增敏效应及其潜在机制。【方法】 CCK8法检测不同浓度124005对人鼻咽癌CNE1细胞的抑制作用,计算IC50值,选择低于IC50的药物浓度作为增敏浓度,集落形成实验检测放射线加或不加124005以及124005+放射线组联合或不联合自噬抑制剂3-MA对CNE1细胞生存曲线的影响,间接免疫荧光法检测γ-H2AX观察DNA损伤修复和GFP-LC3转染CNE1中自噬标志物LC3的聚集,流式细胞术检测细胞周期分布和凋亡、免疫印迹法 ...
doaj
Application of pyroptosis score in the treatment and prognosis evaluation of gastric cancer. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2023 Li L, Liao A.
europepmc +1 more source
Investigating the Effects of Camellia petelotii Chi Extract on Nonalcoholic Fatty Liver Disease Based on TLR-4/NF-κB Signaling Pathway [PDF]
To examine the effects and mechanisms of Camellia petelotii Chi extract on the rat model of non-alcoholic fatty liver disease (NAFLD), a set of ten SD rats was randomly chosen as the blank group, while the remaining 50 rats were given a high-sugar, high ...
Chunping TANG +7 more
core +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban脊髓损伤（SCI）作为一种常见的神经系统疾病，主要由机械性创伤引起，通常会导致患者出现大范围的感觉、运动和自主神经损伤，严重影响患者生存质量。近年来研究发现，凋亡、坏死性凋亡、自噬、焦亡、铁死亡以及铜死亡等调节性细胞死亡（RCD）的发生机制与SCI的发生发展密切相关，且各RCD之间还存在一定的串扰关系。因此，本文将对SCI发病过程中与RCD相关的机制和基于细胞死亡抑制剂、外泌体和组织工程疗法治疗SCI的最新研究进展进行综述，以期为未来SCI相关基础研究及临床治疗提供参考。
赵依婷, 秦合伟
doaj