Zhongshan Daxue xuebao. Yixue kexue ban, 2011 【目的】 探究一种高效稳定分离、扩增人脐带间充质干细胞（hUC-MSCs）的方法。【方法】取材脐带20条，获得Wharton’s Jelly组织，分别用酶联合消化法（12例）和酶序贯消化法（8例）来分离、提取hUC-MSCs，比较两种方法分离hUC-MSCs的成功率、原代培养时间及获得的细胞数量；利用低糖DMEM（LG-DMEM）完全培养基和LD-Mesen培养基（MesenPro RSTM与LG-DMEM的混合培养基）分别培养hUC-MSCs，比较两种培养体系的扩增效率 ...
doaj
[A prospective study on application of human umbilical cord mesenchymal stem cells combined with autologous Meek microskin transplantation in patients with extensive burns]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi, 2023 Yan TT +15 more
europepmc +1 more source
Fe3O4@PDA标记的脐带间充质干细胞对高糖环境下肾小管上皮细胞自噬的影响
Zhongguo shiyan zhenduanxue目的分析Fe3O4@PDA标记的脐带间充质干细胞(hUCMSCs) 在高糖（HG）环境下对肾小管上皮细胞（HK2细胞）的影响，探讨其对HG环境下HK2细胞自噬的作用机制。方法合成Fe3O4@PDA纳米粒子（Fe3O4@PDA NPs），培养hUCMSCs、HK2细胞，将HK2细胞分为4组，包括①对照组②HG组③Fe3O4@PDA hUCMSCs组④hUCMSCs组。通过CCK8检测各组HK2细胞活力，Western blot检测各组HK2细胞中自噬相关蛋白Beclin1和p62的表达水平，RT ...
张金妹 +4 more
doaj
人脐带间充质干细胞治疗大鼠输卵管炎对血清炎症因子及 输卵管结构功能的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 摘 要： 【目的】 为了寻找治疗输卵管性不孕的有效方法，探讨人脐带间充质干细胞（ＷＪＭＳＣ）治疗输卵管炎的应用前景。【方法】 成年雌性Ｗｉｓｔａｒ大鼠５０只，随机分为模型组（４０只）和对照组（１０只）。模型组大鼠双侧输卵管注入混合菌后，再随机分成５组；急性治疗前组（５只）于造模后第２０天取材；急性治疗组（１０只）于造模第２０天，给予腹腔注射ＷＪＭＳＣ（１ × １０６／ｍＬ）， ７ ｄ一次，共３次，疗程结束１周后５只取材；慢性治疗前组（５只）于造模后３０ ｄ 取材；慢性治疗组（１０只 ...
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Nurr1基因修饰人脐血间充质干细胞源性多巴胺能 神经元移植治疗帕金森病
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 探讨Nurr1基因修饰的人脐血间充质干细胞(HUCB-MSC)源性的多巴胺能神经元移植对帕金森病(PD)模型鼠的治疗作用.【方法】 SD大鼠PD模型随机分为假手术组(A组)?单纯HUCB-MSC组(B组)、Nurr1基因修饰HUCB-MSC组(C组)。将HUCB-MSC及Nurr1基因修饰后的HUCB-MSC体外分化为多巴胺能神经元,并通过立体定向的手段将其移植入毁损侧纹状体,观察移植前后PD模型大鼠旋转行为的变化和脑内多巴胺(DA)含量的改变,以及纹状体区酪氨酸羟化酶 (TH) 表达的变化。【
doaj
[Human umbilical cord mesenchymal stem cells protect against neonatal white matter injury by activating the Nrf2/Keap1/HO-1 signaling pathway]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiWang C, Wang MX, Zhu YP.
europepmc +1 more source
[Chitosan hydrogel loaded with human umbilical cord mesenchymal stem cell-derived exosomes promotes healing of chronic diabetic wounds in rats]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoQiu X, Wang M, Tang J, Zhou J, Jin C.
europepmc +1 more source
[Human umbilical cord mesenchymal stem cell grafting alleviates inflammatory response in type 1 diabetic mice by suppressing M1 macrophage polarization through Chi3l1]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoLiu X, Xu Y, Sheng H, Liu H.
europepmc +1 more source
[Application of umbilical cord mesenchymal stem cells in the treatment of severe immune-mediated thrombocytopenia after allogeneic hematopoietic stem cell transplantation in children]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiZhang B, Luan Z, Tang XF, Wu NH.
europepmc +1 more source
[Role of the nuclear factor-kappa B signaling pathway in the repair of white matter injury in neonatal rats through human umbilical cord mesenchymal stem cell transplantation]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiZhang SJ +4 more
europepmc +1 more source