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随机引物引导的乙型肝炎病毒DNA探针标记和重复杂交

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 1992
随机引物引导的探针标记足一种比切中移位更为有效的探针标记方法,作者应用这种方法制备32P标记的乙型肝炎病毒(HBV)DNA探针。经液闪测定,探什比放射性为lxl09cpm/ug,(32)P掺入率为71.43%,均比切口移位有关参数上限高出数倍。将这种探针用于尼龙膜载样杂交,在每100cm2的样膜所加探针放射性强度为5 x106cpm、浓度为20ug/L条件下,放射自显影24小时即可出示清晰结果。并且检出HBV DNA灵敏度达0.1Pg,检测HBSAg和HBCAg双阳性血清,HBVDNA阳性率达87 ...
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[Progress in metabolomics of multiple myeloma]. [PDF]

open access: yesZhonghua Xue Ye Xue Za Zhi
Ye X, Sun ZX, Qu XY, Fan L.
europepmc   +1 more source

[Extracellular matrix materials and tissue regeneration and repair]. [PDF]

open access: yesZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Zhao L, Xie H.
europepmc   +1 more source

[Effect and mechanism of astaxanthin on the aging of high glucose-treated human skin fibroblasts]. [PDF]

open access: yesZhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Tang LJ   +6 more
europepmc   +1 more source

[Research progress on lactylation modification in pathogenesis of osteoarthritis]. [PDF]

open access: yesZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Wu Y   +7 more
europepmc   +1 more source

[Research progress on cellular metabolic reprogramming in skin fibrosis]. [PDF]

open access: yesZhejiang Da Xue Xue Bao Yi Xue Ban
Qian S, Dai S, Guo C, Xu J.
europepmc   +1 more source

[Research progress on the quercetin target role and signaling pathway in anti-liver fibrosis]. [PDF]

open access: yesZhonghua Gan Zang Bing Za Zhi
Zhao RQ   +9 more
europepmc   +1 more source

[Pathogenic mechanisms of Leber hereditary optic neuropathy caused by m.3472T>C mutation]. [PDF]

open access: yesZhejiang Da Xue Xue Bao Yi Xue Ban
Zhang H, Shan W, Yasheng M, Guan M.
europepmc   +1 more source

[Application and advances of exosome-hydrogel system in wound healing]. [PDF]

open access: yesZhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Qiu Y, Tan Q.
europepmc   +1 more source

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