Results 51 to 60 of about 822,650 (184)
, 2006 表达方法是知识的创造和发展的一个重要方面,对知识的承传也有重要影响。技术知识的图像表达与绘画艺术密切相关。中国画家在以再现视觉效果为追求的对位置向背方面的揣摸及绘画实践中总结出了一些与现在的透视理论一致的绘画方法,并将这些方法用于机械、器物的绘画之中。晋代,数学家已经运用文字和图来阐释数学知识,地图专家总结了绘图的法则。唐代画家在描绘台阁、车舆、器物等时追求“形似”,敦煌壁画多具有纵向的移动透视或散点透视画法的特征,间有相当于一点透视的画法 ...
田淼, 张柏春
core
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Microstructural characterization of Li0.32La0.56Fe0.01Ti0.94Sb0.06O3 (LLFeTSO) using AC‐TEM revealed that Fe3+/Sb3+ ion co‐doping induces the migration of lattice oxygen to grain boundaries (GBs), enhancing GB conductivity. However, the atomic arrangement at the GBs remains highly ordered and lacks sufficient cation vacancy carriers.
Binxuan Jiang +8 more
wiley +1 more source
, 2003 探讨人肝癌细胞(SMMC-7721)的同步化方法及粘附分子integrin β_1 在不同周期肝癌细胞上的表达。采用胸腺嘧啶脱氧核苷、秋水仙碱顺序阻断和胸腺嘧啶脱氧核苷双阻断后释放培养的方法分别获得G_1期和S期的肝癌细胞,用流式细胞仪进行周期分析;采用抗体阻断技术和流式细胞仪检测肝癌细胞上integrin β_1的表达。结果发现未同步化肝癌细胞integrin β_1表达的荧光强度为95.70;G_1期和S期细胞的同步率分别为74.09％和98.29％,integrin β_1表达的荧光强度相应为76.
赵彦华 +4 more
core +1 more source
拓扑异构酶2α受抑癌基因p53的调控并调节前列腺癌细胞的增殖能力
Zhongguo shiyan zhenduanxue, 2017 目的观察TOP2α对前列腺癌细胞增殖能力的影响以及抑癌基因p53对TOP2α的调控能力。方法以Lipofectamine2000试剂转染针对p53和非特异性对照组的siRNA,干扰前列腺癌细胞C4-2中p53的表达,采用Realtime PCR和Western Blot检测细胞内TOP2α的mRNA和蛋白水平表达变化;采用质粒转染法在293T细胞中过表达p53后检测TOP2α蛋白水平的变化,明确p53对TOP2α表达的影响 ...
梁婷婷, 王永坤, 王尧
doaj
, 2005 从籼稻川75A(OryzasativaL.)黄化苗叶片中直接提取基因组总DNA.根据文献报道的水稻花药绒毡层特异启动子Osg6B序列设计引物,采用TouchdownPCR技术获得水稻花药绒毡层特异表达的启动子Tsp1,将该片段克隆在载体pMD18-TVector上转化到大肠杆菌JM109,并酶切和PCR验证.序列分析发现,该片段与Osg6B启动子同源性为96%,且具有真核生物启动子的多种特征序列,表明启动子Tsp1为来源于籼稻川75A中的水稻花药绒毡层特异表达的启动子 ...
陈荣军 +4 more
core
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Regulating oxygen vacancies (Ov) in SnO2 to facilitate high‐efficiency carbon dioxide electroreduction reduction reaction (CO2RR) is still a significant challenge. Therefore, a strategy based on Ag‐induced interfacial electron transfer was proposed to modulate Ov in SnO2 for CO2 electroreduction. The prepared Ag/SnO2 with an optimal Ov content
Haoyu Qi +7 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2008 目的探讨乳腺癌ER、PR、Her-2表达与临床病理特征的相关性。方法采用回顾性分析,统计383例乳腺癌患者ER、PR、Her-2的表达;分析ER、PR、Her-2表达的相关性;及其与乳腺癌临床病理特征的关系。结果①ER、PR、Her-2阳性率分别为60.4%、64.1%、17.2%。②ER与PR表达呈正相关;ER、PR分别与Her-2表达负相关;Her-2与ER、PR共同表达呈负相关 ...
郭欢续, 白欧
doaj
miR-497-5p靶向WWP1调控人瘢痕疙瘩成纤维细胞的增殖和凋亡
Zhongguo shiyan zhenduanxue, 2020 目的探讨微小RNA-497-5p(miR-497-5p)是否通过靶向含有WW结构域的E3泛素蛋白连接酶1(WWP1)调控人瘢痕疙瘩成纤维细胞的增殖和凋亡。方法实时荧光定量PCR(qPCR)和蛋白质印迹法(Western blot)测定瘢痕疙瘩成纤维细胞(KFB)和正常皮肤成纤维细胞(NFB)中miR-497-5p、WWP1 mRNA和蛋白表达。TargetScan预测miR-497-5p和WWP1的靶向关系,双荧光素酶报告和Western blot进一步验证。构建miR-497 ...
高栋梁, 高东东, 池凯, 杨喜明
doaj
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT Biomass‐based hydrogels are widely regarded as ideal substrates for self‐powered wearable sensors. However, the practical applications of hydrogels are commonly limited by their inherent low strength and toughness, which restrict their capacity to withstand repeated stretching, bending and other mechanical loads arising from human motion in ...
Boyu Du +9 more
wiley +1 more source
Rare Metals, Volume 45, Issue 10, October 2026.ABSTRACT With the depletion of energy resources and the vigorous development of renewable energy technologies, the demand for efficient energy storage technologies has become increasingly urgent. Dielectric ceramics feature ultrafast charge–discharge speed, but low energy density and inferior efficiency hinder device miniaturization.
Junxuan Liu +14 more
wiley +1 more source