Results 61 to 70 of about 70,351 (171)
乳腺癌生存期相关长非编码 MAPT-AS1 及其共表达基因筛选与验证
Zhongshan Daxue xuebao. Yixue kexue ban, 2019 【目的】筛选乳腺癌患者生存期相关差异表达长链非编码RNA（lncRNA）及其共表达基因，并验证其乳腺癌细胞中的表达情况。【方法】通过TCGA数据库筛选943例RNA-seq数据信息：（837例乳腺癌患者+106例正常对照），发现长非编码MAPT-AS1高表达，乳腺癌患者生存期更长。构建长非编码MAPT-AS1过表达和干扰质粒，将构建好的质粒转染乳腺癌细胞株T47D，并用嘌呤霉素筛选出稳定表达的T47D细胞株，通过RT-qPCR验证长非编码MAPT-AS1及其共表达基因的表达情况。【结果 ...
许家瑞 +6 more
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Study on the mechanisms of Pygopus-2 promoting invasion and metastasis in hepatocellular carcinoma [PDF]
, 2016 第一部分Pygopus-2在HCC中高表达与病人恶性预后密切相关 Pygopus-2是经典Wnt/β-Catenin信号通路转录复合体的重要成分。本研究目的是观察在原发性肝癌中Pygopus-2的表达情况及相关的临床病理意义。RT-PCR的方法检测50对肝癌病人组织标本中癌组织与癌旁组织中Pygopus-2的mRNA表达情况，结果发现癌组织中Pygopus-2的mRNA水平明显高于癌旁组织(P=0.009)。免疫组化分析显示异常表达的Pygopus-2蛋白与肝癌病人的年龄(P=0.025)、肿瘤大小(P=
李杰
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Rare Metals, Volume 45, Issue 5, May 2026.ABSTRACT The inherent brittleness of hardmetals stems from the rigid covalent bond in the ceramic matrix and the sharp ceramic/metal interface, which restricts the nucleation and motion of dislocations. In this study, a novel strategy is proposed to activate significant dislocation behaviors in the WC matrix by employing a non‐equiatomic CoNiFeCr ...
Cheng Qian +9 more
wiley +1 more source
烧伤创面内表皮生长因子、表皮生长因子受体、C-myc三种基因表达变化的临床观察
Zhongguo shiyan zhenduanxue, 2010 目的为探讨烧伤创面愈合机理,了解烧伤创面内表皮生长因子基因、表皮生长因子受体基因和C-myc基因的变化,为临床治疗提供依据。方法我们对25例病人烧伤创面的不同部位应用原位杂交方法,观察表皮生长因子基因、表皮生长因子受体基因和C-myc基因在烧伤创面肉芽中心部（肉芽中心部）、肉芽与愈合皮肤的交界部（创面交界部）、愈合皮肤部位（创面愈合部）和自身正常皮肤对照部位内表达强度和分布的特点。结果同一部位烧伤创面内,表皮生长因子基因和C-myc基因在交界部表达最强;烧伤创面中心部位表达次之,烧伤创面愈合部位表达较弱;
谷廷敏 +4 more
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FOXN2通过PI3K/AKT信号通路对肾癌细胞增殖、凋亡的影响
Zhongguo shiyan zhenduanxue, 2021 目的研究FOXN2通过PI3K/AKT信号通路对肾癌增殖、凋亡的影响。方法实验分FOXN2过表达组、空载体对照组、对照组三组;检测FOXN2在人正常肾上皮细胞HK-2和肾癌细胞786-O中的表达;检测FOXN2过表达组、空载体对照组、对照组细胞的增殖情况和凋亡情况;检测FOXN2过表达组、空载体对照组、对照组细胞PI3K、AKT、p-AKT、Bcl-2、Bax、CDK1、CyclinD1蛋白表达水平。结果 (1)与人正常肾上皮细胞HK-2相比,人肾癌细胞786 ...
李秀文, 赵桂香
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A study about the impact of overpression of Oct4 and Sox2 gene on gastric cancer cell biology and oncology characteristics [PDF]
, 2015 目的：通过构建过表达干细胞因子Oct4和Sox2的胃癌SGC7901细胞株，研究过表达干细胞因子Oct4和Sox2对SGC7901胃癌细胞干细胞特性、肿瘤学特性、及生物学特性的影响，并探讨Oct4及Sox2在肿瘤细胞中的作用机制。 方法：1.构建携带Sox2基因的重组质粒，慢病毒载体感染SGC7901-OCT4细胞株得到SGC7901-OCT4-SOX2稳转细胞株。2.利用荧光定量PCR、western–bloting检测过表达Oct4及Sox2的胃癌SGC7901细胞中胃癌干细胞标志基因表达情况。3 ...
李文鹏
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Attainment Pairing Effects on Cognitive Conflict in Technology‐enhanced EFL Cooperative Tasks
International Journal of Applied Linguistics, Volume 36, Issue 2, Page 1594-1605, May 2026.ABSTRACT This study examines how attainment pairing (high‐high versus high‐low versus low‐low attainment) impacts English as a Foreign Language (EFL) learner engagement during technology‐enhanced cooperative writing tasks. Seventy‐eight Chinese university learners formed 39 dyads (13 pairs per group) via Tencent Meeting.
Ying Liu, Allen Thurston
wiley +1 more source
Expression and Clinical Relevance of uPA and ET-1 in Non-small Cell Lung Cancer [PDF]
, 2011 Background and objective uPA and ET-1 proteins have been reported to be up-regulated in some of human cancers. The aim of this study is to investigate the alteration and clinical relevance of uPA and ET-1 protein levels in non-small cell lung cancer ...
Boshi WANG +7 more
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Focusing Events, Agenda Setting, and Narrative Numbing
Review of Policy Research, Volume 43, Issue 3, May 2026.ABSTRACT Focusing events are important in understanding policy change, as demonstrated in Birkland's work on agenda setting, the multiple streams framework, and the narrative policy framework. These frameworks emphasize the role of focusing events in drawing public attention to broader issues.
Megan K. Warnement Wrobel +1 more
wiley +1 more source
Fault diagnosis and fault-tolerant control for nonlinear systems with linear output structure [PDF]
, 2005 Article describes the process of fault diagnosis and fault-tolerant control for nonlinear systems with linear output ...
Shen, L., Wang, H., Yue, H.
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