Results 11 to 20 of about 6,107 (167)
In vitro culture, cryopreservation, and field reintroduction of the endangered Mingan thistle
Conservation Biology, EarlyView.Abstract Current plant conservation efforts are hindered by, for example, poor seed germination, low viability, and insufficient propagation and preservation technologies. To address these problems, we devised an approach to plant conservation that integrates conservation, preservation, and restoration (CPR), which uses advanced in vitro techniques. We
Mukund R. Shukla +4 more
wiley +1 more source
Genetic diversity of Noctiluca scintillans [PDF]
, 2016 夜光藻是一种常见的赤潮藻，在世界大部分近海海域均有分布。目前普遍认为夜光藻只有一个种，但还没有研究探索不同地理种群的夜光藻是否产生了种内变异。本研究通过单细胞PCR以及单细胞克隆文库的方法，比较了中国黄海、东海、南海，墨西哥湾以及阿拉伯海5个海域夜光藻核糖体基因的遗传多样性。主要研究结果如下： 1、发现22种ITS单倍型(n=103)，12种28S单倍型(n=25)，6种18S单倍型(n=31)；这3个基因的单倍型多样度(Hd)分别为0.824、0.670、0.557，核苷酸多样度(π)分别为0 ...
潘永柏
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Evaluating past and future contributions of conservation programs to species recovery
Conservation Biology, EarlyView.Abstract Impact evaluation of conservation actions is a crucial step in global efforts to curb the biodiversity crisis. Through robust impact evaluation, practitioners can assess the effectiveness of conservation strategies and optimize the use of limited resources.
Rebeca E. Young +10 more
wiley +1 more source
Zhejiang Daxue xuebao. Lixue ban, 2019 大花无柱兰是一种珍稀兰科植物，具有一定的观赏和药用价值，但数量十分稀少，该物种亟待保护。本研究采用SRAP 分子标记技术，对10 个居群的115 份DNA 样品进行PCR 扩增，并开展遗传多样性分析。从81 对引物中筛选出9 个条带清晰、多态性好、重复性高的引物组合，共扩增得到305 条谱带。在物种水平上，多态性比率（PPB） 为100%，Nei’s 基因多样性指数（H） 为0.209 8，Shannon’s 指数（I） 为0.340 2；在居群水平上，PPB 为24.59%～52.13%，H 为0 ...
ZHOUMengying(周梦莹) +6 more
doaj +1 more source
, 2012 利用泥鳅(Misgurnus anguillicaudatus)的微卫星引物跨种扩增大鳞副泥鳅(Paramisgurnus dabryanus),通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167～4.833,平均观测杂合度(Ho)在0.248～0.417,平均期望杂合度(He)在0.379～0.505,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用UPGMA法对7个群体基于DA遗传距离进行聚类,
俞小牧, 游翠红, 童金苟
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Veterinary Dermatology, EarlyView.ABSTRACT Background Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect ≤ 15% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally ...
Xavier Langon +5 more
wiley +1 more source
Genetic diversity on the Genus Skeletonema (Bacillariophyceae) in the coastal waters of China [PDF]
, 2015 骨条藻（Skeletonema）是一类广泛分布于沿海水域的浮游硅藻，也是常见的赤潮生物。目前，对于整个中国沿海骨条藻的分类和分子生物学方面的研究，比较分散，还不能很好地回答我国沿海骨条藻的种类多样性和遗传多样性的问题。因此，本文选择我国沿海的代表性海区，进行周年采样，对分离培养的纯种株系，选择核糖体LSUnrDNA和ITS片段（5.8SrDNA及转录间隔区ITS1、ITS2）对其进行遗传多样性及系统进化分析，旨在从分子生物学方面分析我国沿海骨条藻属的种内、种间和地理株系间的差异，以及系统进化关系 ...
王琰
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Genetic origins and climate‐induced erosion in economically important Asian walnuts
Conservation Biology, Volume 40, Issue 1, February 2026.Abstract The global climate is undergoing unprecedented changes, posing significant threats to species persistence. However, the spatiotemporal impacts on genetic diversity remain poorly understood, hindering species conservation and management. Walnuts, generally referred to as Juglans regia and J. sigillata, are economically vital in Asia, but little
Peng‐Zhen Fan +17 more
wiley +1 more source
基于mtDNA D-loop区和Cyt b基因序列对鹅喉羚南疆亚种遗传多样性与系统进化地位的分析
野生动物学报, 2022 为进一步明确南疆地区鹅喉羚(Gazella subgutturosa)的遗传分化、遗传多样性水平和系统进化地位,以鹅喉羚南疆亚种(G.s.yarkandensis)为研究对象,运用PCR扩增和测序技术,研究mtDNA D-loop区和mtDNA Cyt b基因的序列特征,利用邻接法构建系统发育树和中介网络关系,以此阐明鹅喉羚南疆亚种的遗传多样性水平和系统进化地位。结果表明:鹅喉羚南疆亚种mtDNA D-loop区序列碱基A、T含量为60.38%,存在23个多态位点,占总位点数的2.90 ...
张永浩 韦庆浩 郭丽君 阿布都热合曼·吐尔逊 王玉涛
doaj
, 2010 为认识我国淡水鱼类烂鳃病的病原以及柱形病在我国的发生情况,实验从发生烂鳃病的病鱼中分离细菌性病原,经过生理生化特性分析以及是否在含托普霉素的Shieh培养基中生长并形成黄色假根状菌落,是否产生降解明胶和硫酸软骨素的酶类等特性的鉴定,并结合16SrDNA序列分析,证实柱状黄杆菌(Flavobacterium columnare)是所分离的烂鳃病的病原。同时,研究也证实20世纪曾经命名为烂鳃(Gill-rot)病病原的鱼害黏球菌(Myxococcus piscicola Lu,Nie & Ko,1975 ...
姚卫建 +7 more
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