Results 11 to 20 of about 6,816 (167)

Effects of Oxytetracycline on Population Growth and Genetic Diversity of Euplotesvannus (Marine Protist) [PDF]

open access: yes, 2016
抗生素作为一类环境污染物在海岸带生境中广泛存在,它们对海洋原生生物遗传多样性的影响还不清楚.本工作以一种海洋纤毛虫原生生物(扇形游仆虫(Euplotes vannus))为例,研究了一种常见四环类抗生素(土霉素)对其生长的影响,并通过单细胞(个体)分析,探索了抗生素对其大核基因组中核糖体小亚基RNA基因(SSU r DNA)序列多样性的影响.结果表明,与对照组相比,3个土霉素处理组(1,10和20μg/m L)中纤毛虫的自然增长率随土霉素浓度升高显著降低,细胞大小则呈增大趋势.SSU r ...
付娆   +3 more
core   +1 more source

Using miniaturized laboratory equipment and DNA barcoding to improve conservation genetics training and identify illegally traded species

open access: yesConservation Biology, EarlyView.
Abstract Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high‐throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades.
Maria Joana Ferreira da Silva   +9 more
wiley   +1 more source

The genetic differentiation and mating system of Avicennia marina populations in China [PDF]

open access: yes, 2016
本研究利用SSR分子标记技术,以海南三亚河口、湛江特呈岛和泉州洛阳桥三个地区的白骨壤(Avicenniamarina)种群为研究对象,比较分析三个白骨壤种群的遗传多样性、种群之间的遗传分化和繁育系统模式(包括花粉流、异交率和种子流)。主要结果为: 1、10对核基因组SSR引物在742个个体检测到58个等位基因位点。每个引物扩增等位基因数在5-9个之间,平均5.8个。三个地区种群共出现了15个私有等位基因位点,在等位基因水平上已发现较大的分化与遗传距离。 2、湛江白骨壤种群遗传多样性水平最高 ...
侯蕊
core  

In vitro culture, cryopreservation, and field reintroduction of the endangered Mingan thistle

open access: yesConservation Biology, EarlyView.
Abstract Current plant conservation efforts are hindered by, for example, poor seed germination, low viability, and insufficient propagation and preservation technologies. To address these problems, we devised an approach to plant conservation that integrates conservation, preservation, and restoration (CPR), which uses advanced in vitro techniques. We
Mukund R. Shukla   +4 more
wiley   +1 more source

Genetic diversity of Noctiluca scintillans [PDF]

open access: yes, 2016
夜光藻是一种常见的赤潮藻,在世界大部分近海海域均有分布。目前普遍认为夜光藻只有一个种,但还没有研究探索不同地理种群的夜光藻是否产生了种内变异。本研究通过单细胞PCR以及单细胞克隆文库的方法,比较了中国黄海、东海、南海,墨西哥湾以及阿拉伯海5个海域夜光藻核糖体基因的遗传多样性。主要研究结果如下: 1、发现22种ITS单倍型(n=103),12种28S单倍型(n=25),6种18S单倍型(n=31);这3个基因的单倍型多样度(Hd)分别为0.824、0.670、0.557,核苷酸多样度(π)分别为0 ...
潘永柏
core  

Evaluating past and future contributions of conservation programs to species recovery

open access: yesConservation Biology, EarlyView.
Abstract Impact evaluation of conservation actions is a crucial step in global efforts to curb the biodiversity crisis. Through robust impact evaluation, practitioners can assess the effectiveness of conservation strategies and optimize the use of limited resources.
Rebeca E. Young   +10 more
wiley   +1 more source

大鳞副泥鳅7个群体遗传变异的微卫星分析 [PDF]

open access: yes, 2012
利用泥鳅(Misgurnus anguillicaudatus)的微卫星引物跨种扩增大鳞副泥鳅(Paramisgurnus dabryanus),通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167~4.833,平均观测杂合度(Ho)在0.248~0.417,平均期望杂合度(He)在0.379~0.505,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用UPGMA法对7个群体基于DA遗传距离进行聚类,
俞小牧, 游翠红, 童金苟
core  

Assessing D‐Squame as a Minimally Invasive Technique to Evaluate the Cutaneous Immune Response mRNA in a Dog Model of Canine Atopic Dermatitis

open access: yesVeterinary Dermatology, EarlyView.
ABSTRACT Background Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect ≤ 15% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally ...
Xavier Langon   +5 more
wiley   +1 more source

Genetic diversity on the Genus Skeletonema (Bacillariophyceae) in the coastal waters of China [PDF]

open access: yes, 2015
骨条藻(Skeletonema)是一类广泛分布于沿海水域的浮游硅藻,也是常见的赤潮生物。目前,对于整个中国沿海骨条藻的分类和分子生物学方面的研究,比较分散,还不能很好地回答我国沿海骨条藻的种类多样性和遗传多样性的问题。因此,本文选择我国沿海的代表性海区,进行周年采样,对分离培养的纯种株系,选择核糖体LSUnrDNA和ITS片段(5.8SrDNA及转录间隔区ITS1、ITS2)对其进行遗传多样性及系统进化分析,旨在从分子生物学方面分析我国沿海骨条藻属的种内、种间和地理株系间的差异,以及系统进化关系 ...
王琰
core  

Genetic origins and climate‐induced erosion in economically important Asian walnuts

open access: yesConservation Biology, Volume 40, Issue 1, February 2026.
Abstract The global climate is undergoing unprecedented changes, posing significant threats to species persistence. However, the spatiotemporal impacts on genetic diversity remain poorly understood, hindering species conservation and management. Walnuts, generally referred to as Juglans regia and J. sigillata, are economically vital in Asia, but little
Peng‐Zhen Fan   +17 more
wiley   +1 more source

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