Results 31 to 40 of about 19,100 (167)
Characterization and function analysis of DNA replication enzymes from Hyperthermophilic archaea Palaeococcus pacificus DY20341 [PDF]
, 2016 DNA是至今发现的绝大多数生物遗传信息的携带者。DNA复制机制保证了细胞分裂期间遗传信息的复制和传递，是维持生物遗传稳定性的前提。DNA复制过程涉及几十种蛋白和酶的参与以确保遗传信息被准确而及时的复制。 研究DNA复制对人类疾病，特别是肿瘤及传染病的机理探索有至关重要的意义。然而参与真核生物(包括人类)DNA复制的酶系非常复杂，对真核生物DNA复制机制的研究面临着诸多挑战。细菌的DNA复制系统相对简单，但其复制酶系与真核生物差别较大。古菌的复制酶系与真核生物的复制酶系相似，而且相对简单 ...
赵文昭
core
Plant nutrient‐acquisition strategies contribute to species replacement during primary succession
Journal of Ecology, Volume 113, Issue 4, Page 988-1003, April 2025.Pioneer species Hippophae acquires phosphorus and nitrogen mainly by ‘mining’ and N2‐fixing strategies and thus dominates in the beginning of primary succession. As soil nutrient availability increases with the mobilization of apatite‐P and input of N‐rich plant residues, Hippophae' strategies become less efficient than Populus's scavenging strategies.
Xiao‐Long Li +12 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2005 目的 分离获得高产量、高比活性的胶原酶。方法 以溶组织梭菌作为菌种 ,确立最佳培养条件 ;应用胶原悬浮分析法测定胶原酶总活性 ;应用FALGPA胶原酶活性测量法进行比活性分析。结果 获得了高产量、高比活性的胶原酶。结论 通过改良并优化溶组织梭菌培养条件 ,可以获得低成本、高产量、高活性的胶原酶。
常淑芳 +5 more
doaj
Translocation of Mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death [PDF]
, 2016 在最近的研究结果中，混交激酶域蛋白Mlkl(mixedlineagekinasedomain-like protein)被确认为Rip3蛋白在细胞坏死信号通路中的下游蛋白。然而，Mlkl如何 在细胞坏死信号通路中行使怎样的功能，尚未明晰。通过在Mlkl基因敲除的细 胞中回补Mlkl蛋白进行一系列的研究，实验数据表明，Mlkl蛋白氨基端结构域 是其在坏死通路中行使功能的必要部分。在坏死过程中，Mlkl蛋白形成多聚体； 或者在细胞中利用分子生物学手段人为地诱导形成Mlkl蛋白多聚体，也能够引 起细胞坏死 ...
李文娟
core
Veterinary Dermatology, Volume 35, Issue 6, Page 617-625, December 2024.Background – Autosomal recessive ichthyosis leads to structural or biochemical changes that impair skin barrier function. Hypothesis/Objectives – To assess (1) the phenotype and genotype in a litter of Jack Russell Terriers with autosomal recessive congenital ichthyosis (ARCI), and (2) the defective skin barrier and determine if a topical ceramide can ...
Elizabeth Mauldin +7 more
wiley +1 more source
Shuitu Baochi Xuebao, 2011 通过野外调查和室内分析相结合,运用相关分析和通径分析等统计方法,研究酸化梨园不同土壤pH梯度下土壤理化性质与土壤酶活性的关系。结果表明:除蔗糖酶仅在5.0～5.5变化趋势最明显外,过氧化氢酶、酸性磷酸酶和脲酶活性在4个pH梯度4.0～4.5,4.5～5.0,5.0～5.5,5.5～6.0下分别表现出明显的层次差异。同时土壤水分和盐分也表现出明显的空间层次差异。土壤酶活性与土壤温度、水分、盐分、pH、各土壤有效养分间相关性较好。就通径分析表明,土壤pH对过氧化氢酶活性的影响最为显著,对蔗糖酶、酸性磷酸酶 ...
赵静 +6 more
doaj
Functional Ecology, Volume 38, Issue 11, Page 2433-2446, November 2024.Read the free Plain Language Summary for this article on the Journal blog. Abstract Plant phosphorus (P) acquisition strategy is considered to be an intrinsic driver behind plant succession. However, variations in plant P acquisition strategies in connection to soil P fraction changes after wetland drainage remain unclear.
Zhenhui Jiang +6 more
wiley +1 more source
Shuitu Baochi Xuebao, 2011 土壤酶活性的高低可以直接或间接反映土壤肥力和土壤健康程度。研究选取烟台市黄务镇破坏山体周边7种植被恢复模式,测定土壤脲酶、多酚氧化酶、过氧化物酶、过氧化氢酶活性及土壤理化性质和细菌、真菌、放线菌数量。目的是探讨不同植被恢复模式对土壤酶活性的影响机制,为合理恢复植被提供参考依据。结果表明:不同植被恢复模式的土壤酶活性表现为0-10cm土层高于10-20cm土层;对土壤酶活性的改善作用为混交林>纯林>灌草丛,人工植被恢复模式中以黑松麻栎混交林最优;土壤酶活性具有显著差异 ...
张刘东 +5 more
doaj
Protein phosphatase 2A-B55δ enhances chemotherapy sensitivity of human hepatocellular carcinoma under the regulation of microRNA-133b [PDF]
, 2016 目的：原发性肝细胞癌(hepatocellularcarcinoma，HCC)是我国公共卫生关注的热点问题之一，寻找增强HCC化疗敏感性的有效干预靶点具有重要意义。蛋白磷酸酶2A(proteinphosphatase2A，PP2A)是重要的肿瘤抑制因子；PPP2R2D基因编码的PP2A-B55δ亚基参与细胞周期有丝分裂调控。我们前期研究验证了PPP2R2D基因3’-非翻译区(3’-untranslatedregion，3’UTR)的+15G>C多态性位点受微小RNA-133b(microRNA-133b，
庄群瑛
core
Methods in Ecology and Evolution, Volume 15, Issue 8, Page 1408-1421, August 2024.Abstract Environmental DNA (eDNA) monitoring of species distribution has become a critical tool in ecology, conservation biology and fisheries for identifying the presence and distribution of diverse organisms, including important, threatened and invasive species.
Jiwei Yang +4 more
wiley +1 more source