构建MS2-RIP方法用于鉴定lncRNA DANCR在肿瘤细胞中的相互作用分子 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的为了探讨lncRNA DANCR在肿瘤细胞中的作用机制，本研究拟构建一种新的MS2-RIP方法用于鉴定DANCR的相互作用分子。方法利用MS2噬菌体衣壳蛋白可与噬菌体复制酶编码基因5’端一段由19个碱基组成的RNA茎环结构序列，即MS2结合位点（MS2 Binding Site，MS2 BS）高效结合的特点，构建串联表达MS2 BS和DANCR的过量表达质粒，通过MS2蛋白与MS2 BS的亲和作用，使DANCR得到富集，并且同步捕获与DANCR相互作用的分子，用于进一步分析鉴定 ...
巫孟师 +4 more
doaj +2 more sources
PTBP1介导长链非编码RNA RP11-879F14.2发挥抑制心肌纤维化的作用 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的研究长链非编码RNA（lncRNA）RP11-879F14.2调控心肌成纤维细胞纤维化表型的作用及机制。方法对心衰患者及健康对照者心肌组织进行Masson染色检测心肌胶原水平。lncRNA表达谱芯片检测心衰患者及健康对照者心肌中lncRNAs的表达变化，实时定量荧光PCR（RT-qPCR）验证RP11-879F14.2在心衰患者心肌中的表达。利用重组RP11-879F14.2腺病毒（rAd-RP11-879F14.2）感染人心房肌成纤维细胞（HAFs），检测纤维化相关基因Col1a1 ...
杨莹 +7 more
doaj +2 more sources
Construction and validation of prognostic risk model for hepatocellular carcinoma based on biological analysis of palmitoyl-associated enzyme long-chain non-coding RNA [PDF]
, 2023 Objective·To explore the effect of screening the expression of long non-coding RNA (lncRNA) related to palmitoylation on prognosis of liver cancer based on The Cancer Genome Atlas (TCGA) database and construct a risk prediction model in liver cancer ...
LI Yahui +4 more
core +1 more source
TGF-β1/SMAD在糖尿病肾病中的作用机制与研究进展 [PDF]
, 2023 Diabetic nephropathy (DN) is a common complication of diabetes and a leading cause of end-stage renal disease. Transforming growth factor-β1 (TGF-β1)/SMAD signaling activation plays an important role in the onset and progression of DN.
Chen, Haiyong +4 more
core +1 more source
Effects of lncRNA PKD2-2-3 on cell proliferation, clone formation, migration, and invasion of lung adenocarcinoma [PDF]
, 2023 Background and purpose: Long non-coding RNA (lncRNA) is abnormally expressed in lung adenocarcinoma patients, and closely related to tumor occurrence, development and chemotherapy resistance.
JIA Liqing, GE Xiaolu, JIANG Lin, ZHOU Xiaoyan
core +1 more source
Zhongguo shiyan zhenduanxue, 2022 长链非编码 RNA(Long non-coding RNA,LncRNA)位于细胞质或细胞核中，长度超过200nt，是一类非编码 RNA 分子，可通过结合表观遗传蛋白修饰其靶基因；与 mRNA 碱基互补结合，抑制翻译和降解；作为ceRNA 竞争性结合 microRNA 等方式调控基因的表达，参与调节机体的各项生命活动[1-2]。
钟剑锋, 钟莉莉, 于双
doaj
Research progress in the relationship between PREX1 gene and tumor pathogenesis [PDF]
, 2023 Phosphatidylinositol-3， 4， 5-triphosphate-dependent Rac exchange factor 1 （PREX1） is a coding gene that is abnormally expressed in a variety of tumors. As a guanine nucleotide exchange factor （RacGEF） of small GTP binding protein Rac1， PREX1 gene-encoded
Chen Ting, Zhou Yu
core +1 more source
Advances in Chinese medicine-mediated AMPK/mTOR signaling pathway for the treatment of osteoporosis [PDF]
, 2023 Osteoporosis （OP） is a systemic bone disease and a common orthopedic disorder in elderly patients. High morbidity， disability and mortality rates and medical cost of OP bring huge burden to the patients and their family.
Zhang Wenhao, Yi Lin, Xiang Wenyuan, Remira·Aimaiti, Deng Yingjie, Liao Jun, Liang Zhiquan, Li Leijiang, Fang Rui
core +1 more source
Mechanism of alternative splicing in gene transcription and research progress in head and neck squamous cell carcinoma [PDF]
, 2023 Misregulation of alternative splicing may result in splicing defects in different types of cancers under multiple pathological conditions. Alternative splicing events may serve as potential molecular markers during the diagnosis and treatment for cancer.
Meng Guangxue, Ma Hong, Liao Dezhong
core +1 more source
Circular RNA CircHIPK3 Promotes NCI-H1299 and NCI-H2170 Cell Proliferation through miR-379 and its Target IGF1 [PDF]
, 2017 背景与目的已有的研究证明:环状RNA是一类在哺乳动物中普遍存在的具有稳定闭合环状结构的内源性RNA分子。环状RNA; circHIPK3(circular RNA HIPK3, circHIPK3)在肝细胞癌(hepatocellular carcinoma,; HCC)中表达水平较高,促进肝癌细胞生长。但是其在非小细胞肺癌(non-small cell lung cancer,; NSCLC)中的作用及其调控机制尚无文献报道。本研究拟探讨环状RNA; circHIPK3对NSCLC细胞系NCI ...
朱学军, 王云, 田芳, 肖哲
core +1 more source