Results 41 to 50 of about 10,357 (168)
Integrative Conservation, Volume 4, Issue 3, Page 277-286, September 2025.This study provides an innovation to address the challenge in accessibility to samples and genetic markers in Snow leopard conservation genetic studies. We performed next‐generation sequencing (NGS) on 19 fecal samples obtained from the eastern Qinghai‐Tibet Plateau, and successfully assembled 17 complete mitogenome sequences (~16,720 bp) and ...
Chen Cheng +7 more
wiley +1 more source
The Role of Messenger RNA Alternative Polyadenylation During Developmental Gene Expression Regulation and Adapdation of Rice [PDF]
, 2016 水稻（Oryzasativa）不仅是世界上最重要的粮食作物之一，也是单子叶模式植物。水稻品种japonicaNipponbare和indica93-11为两个水稻亚种代表，广泛的种植于我国的南北区域，对环境与气候形成一定的适应性，在我国形成北粳南籼的生产格局。它们各自具有独自的生理特性和生长周期。转录组为特定生理条件下，细胞中全部转录产物的集合。通过对水稻发育过程转录组的研究，可以全面地了解基因表达调控在水稻发育过程中的调节作用以及对环境适应性。多聚腺苷化是基因在转录形成成熟的转录本之前的加工过程之一 ...
付海辉
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External validation of QUiPP App in three independent European cohorts of symptomatic women
Ultrasound in Obstetrics &Gynecology, Volume 66, Issue 2, Page 163-174, August 2025.ABSTRACT Objective To validate externally the QUantitative Innovation in Predicting Preterm birth (QUiPP) App v.2 for the prediction of spontaneous preterm birth (sPTB) in symptomatic women attending tertiary care in Europe. Methods The QUiPP App v.2 was validated in three independent datasets: a prospective European multicenter cohort across five ...
A. M. Fischer +8 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】获取人蛋白酪氨酸磷酸酯酶4A2（PTP4A2）基因并高效表达纯化,对于产物的体外活性进行测定。【方法】用RT-PCR技术,从肝癌细胞系HepG2的总RNA中,获得编码PTP4A2基因功能片段的DNA。测序后,通过酶切亚克隆至表达载体pGEX-4T-2,构建重组表达载体,并导入大肠杆菌E.coliBL21中,IPTG诱导表达重组的GST融合蛋白。对重组融合蛋白过谷胱甘肽琼脂糖柱进行纯化,western印记进行鉴定,质谱检测蛋白分子量。通过其对底物磷酸多肽的去磷酸化反应,鉴定其活性。【结果 ...
程超 +5 more
doaj
He jishu传统的堆芯计算程序为了提高计算效率，通常采用简化的模型或者松散耦合的方式。然而，随着计算机性能的不断提高，对计算精度的追求越来越高，传统的方法无法更好地满足目前对于计算性能的要求。本研究旨在开发一种采用更精确理论模型、更紧密多物理场耦合方式的堆芯计算程序。采用半解析节块方法、微观燃耗方法和子通道热工水力模型开发了三维多物理堆芯分析程序，并通过程序内部耦合的方式实现中子学计算、热工水力计算以及燃料性能分析计算。同时为了提高计算效率，采用了组件-堆芯改进型两步法。为了验证程序的计算性能 ...
司 胜义 +5 more
doaj +1 more source
Screening of SNP Loci of Growth-related Candidate Gene and Genetic Diversity of Five Populations Based On Microsatellite Markers in Fujian Oyster, Crassostrea angulata [PDF]
, 2015 福建牡蛎(Crassostreaangulata，C.angulata)又名葡萄牙牡蛎，具有重要的经济价值和生态价值，但由于长期未经选育以及人工育苗的应用，导致牡蛎养殖出现经济性状衰退及死亡率逐年增高等现象，亟需加强种质资源保护，开展遗传改良研究。本研究利用单核苷酸多态性分子标记（singlenucleotidepolymorphism，SNP）对福建牡蛎生长相关的5个候选基因，即胰岛素相关多肽（insulinrelatedpeptides，IRPs）、ɑ-淀粉酶（ɑ-Amylase，ɑ-AMY ...
楚瑞敏
core
New Phytologist, Volume 247, Issue 2, Page 504-517, July 2025.Summary Repetitive DNA is a feature of all organisms, ranging from archaea and plants to humans. DNA repeats can be seen both in coding and in noncoding regions of the genome. Due to the recurring nature of the sequences, simple DNA repeats tend to be more prone to errors during replication and repair, resulting in variability in their unit length ...
Sridevi Sureshkumar +3 more
wiley +1 more source
Study on the microbial community structure, diversity and abundance in water-soil interface sediments on the watershed of three rivers [PDF]
, 2013 为了探讨无机盐分、养分及有机质矿化作用对微生物群落结构、多样性和功能微生物丰度影响，河流水土界面微生物群落结构和碳、氮、硫循环功能微生物种群的演替规律，本论文以九龙江、闽江和瓯江三江流域作为研究区域，并增加温室培养实验，对其中水土界面沉积物样品中的理化参数、微生物群落、多样性和功能微生物丰度进行测定；采用基因文库构建技术、变性梯度凝胶电泳技术（DGGE）和illumina高通量测序技术分析了样品中微生物群落结构及活动规律；利用实时荧光定量PCR（RT-PCR）技术对功能微生物进行定量 ...
李虎
core
Ultrasound in Obstetrics &Gynecology, Volume 65, Issue 4, Page 470-477, April 2025.ABSTRACT Objective To evaluate the presence and levels of cytomegalovirus (CMV) cell‐free DNA (cfDNA) fragments in women pregnant with a fetus with symptomatic congenital CMV (cCMV). Methods The study comprised nine women whose fetuses were diagnosed with cCMV between June 2019 and July 2024 at 20 + 4 to 34 + 1 weeks' gestation (n = 8) or neonatally (n
B. H. W. Faas +9 more
wiley +1 more source
Gaoya dianqi, 2016 采用小波变换（wavelet transform,WT）和相关向量机（relevance vector machine,RVM）结合的方法对风电场发电功率短期预测进行研究。先利用小波变换将信号分解为不同频率的子序列,提取出风速中的低频趋势分量和高频波动分量;再结合风向和温度数据,运用RVM在不同分量上分别进行预测,并通过重构得到功率的预测结果。此方法应用于国内某风电场,仿真结果表明,通过小波分析能够把握风速变换规律,RVM预测法有较强的学习能力,小波—相关向量机法有效提高了预测精度,表明了该方法的可行性。
刘雨莎, 杨晓萍, 李锐, 张豪
doaj