Results 31 to 40 of about 38,521 (297)
Metabolites, 2014 13C metabolic flux analysis (MFA) is a tool of metabolic engineering for investigation of in vivo flux distribution. A direct 13C enrichment analysis of intracellular free amino acids (FAAs) is expected to reduce time for labeling experiments of the MFA.
Nobuyuki Okahashi +3 more
doaj +1 more source
ScalaFlux: A scalable approach to quantify fluxes in metabolic subnetworks.
PLoS Computational Biology, 2020 13C-metabolic flux analysis (13C-MFA) allows metabolic fluxes to be quantified in living organisms and is a major tool in biotechnology and systems biology.
Pierre Millard +5 more
doaj +1 more source
Systems biology analysis of drivers underlying hallmarks of cancer cell metabolism. [PDF]
, 2017 Malignant transformation is often accompanied by significant metabolic changes. To identify drivers underlying these changes, we calculated metabolic flux states for the NCI60 cell line collection and correlated the variance between metabolic states of ...
Bordbar, Aarash +5 more
core +1 more source
Rapid Prediction of Bacterial Heterotrophic Fluxomics Using Machine Learning and Constraint Programming. [PDF]
PLoS Computational Biology, 2016 13C metabolic flux analysis (13C-MFA) has been widely used to measure in vivo enzyme reaction rates (i.e., metabolic flux) in microorganisms. Mining the relationship between environmental and genetic factors and metabolic fluxes hidden in existing ...
Stephen Gang Wu +8 more
doaj +1 more source
BCKDH: the missing link in apicomplexan mitochondrial metabolism is required for full virulence of Toxoplasma gondii and Plasmodium berghei [PDF]
, 2014 While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle.
Barrett, Michael P. +11 more
core +1 more source
Multi-objective experimental design for 13 C-based metabolic flux analysis [PDF]
Mathematical Biosciences, 2015 (13)C-based metabolic flux analysis is an excellent technique to resolve fluxes in the central carbon metabolism but costs can be significant when using specialized tracers. This work presents a framework for cost-effective design of (13)C-tracer experiments, illustrated on two different networks.
Bouvin, Jeroen +7 more
openaire +2 more sources
BMC Biology, 2019 The study of tumorigenic rewiring of metabolic flux is at the heart of cancer metabolic research. Here, we review two widely used computational flux inference approaches: isotope tracing coupled with Metabolic Flux Analysis (13C-MFA) and COnstraint-Based
Shoval Lagziel +2 more
doaj +1 more source
A peptide-based method for 13C Metabolic Flux Analysis in microbial communities. [PDF]
PLoS Computational Biology, 2014 The study of intracellular metabolic fluxes and inter-species metabolite exchange for microbial communities is of crucial importance to understand and predict their behaviour. The most authoritative method of measuring intracellular fluxes, 13C Metabolic
Amit Ghosh +7 more
doaj +1 more source
HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium [PDF]
, 2013 Background: While acute lung injury (ALI) contributes significantly to critical illness, it resolves spontaneously in many instances. The majority of patients experiencing ALI require mechanical ventilation.
Bonney, Megan +9 more
core +3 more sources
p13CMFA: Parsimonious 13C metabolic flux analysis.
PLoS Computational Biology, 2019 Deciphering the mechanisms of regulation of metabolic networks subjected to perturbations, including disease states and drug-induced stress, relies on tracing metabolic fluxes.
Carles Foguet +7 more
doaj +1 more source