Results 231 to 240 of about 80,061 (261)

Images of 16S ribosomal RNA by scanning tunnelling microscopy

Journal of Microscopy, 1991
SUMMARYWe report the use of scanning tunnelling microscopy (STM) to study surface topographies of complex nucleic acid structures. From low‐resolution STM images of uncoated 16S ribosomal RNA, we demonstrate the possibility of determining several objective parameters (molecular mass and radius of gyration) in order to characterize and identify the ...
P J, Flamion, C, Cachia, J P, Schreiber, T, David, E, Lesniewska, J P, Goudonnet   +5 more
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Methylation of 16S RNA during ribosome assembly in vitro

Nature, 1974
Methyl deficient 16S RNA from kasugamycin resistant Escherichia coli has been used to study methylation of RNA during ribosome assembly in vitro. Methylation occurs at an intermediate stage and is inhibited at a late stage of assembly. 30S ribosomal proteins required for methylation, and inhibition, have been identified.
P, Thammana, W A, Held
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To Knot or Not to Knot? Examination of 16S Ribosomal RNA Models

Journal of Biomolecular Structure and Dynamics, 1998
The presence of topological knots in large RNA structures is highly unlikely given that 1) no RNA structures determined thus far contain topological knots, 2) secondary structure maps for most RNA molecules are knot free, 3) there are no known RNA topoisomerases, and 4) it is difficult to imagine how knots could be formed specifically and uniquely ...
M S, VanLoock, B A, Harris, S C, Harvey
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Complementary binding of oligonucleotides with 16S RNA and ribosomal ribonucleoproteins

Molecular Biology Reports, 1975
The accessibility of single-stranded sequences in 16S RNA in free state and in ribonucleoprotein particles (RNP) to complementary binding with isoplith fractions of oligonucleotides was studied. RNP had different protein composition and corresponded to intermediate stages of E. coli 30S subunit assembly in vitro.
A M, Kopylov, N V, Chichkova, A A, Boganov, S K, Vasilenko   +3 more
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Structure of synthetic unmethylated 16S Ribosomal RNA as purified RNA and in reconstituted 30S ribosomal subunits

Biochemistry, 1989
16S ribosomal RNA was made by in vitro transcription of a cloned gene, and its structure was compared to authentic 16S ribosomal RNA. The comparison was made by subjecting the two types of 16S rRNA to chemical reagents that react specifically with unpaired bases and determining the extent of reaction by reverse transcription and gel electrophoresis of ...
G, Ericson, K, Chevli, P, Wollenzien
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Comparative Anatomy of 16-S-like Ribosomal RNA

1985
Publisher Summary This chapter examines the range of the variation of secondary structure among the 16-S-like rRNAs. This brings into a larger structural context a recent detailed analysis of the individual helical elements and provides a basis for an accurate alignment of the corresponding regions of different primary structures.
R R, Gutell, B, Weiser, C R, Woese, H F, Noller   +3 more
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Thermodynamics of the pseudo‐knot in helix 18 of 16S ribosomal RNA

Biopolymers, 2018
AbstractA fragment of E. coli 16S rRNA formed by nucleotides 500 to 545 is termed helix 18. Nucleotides 505‐507 and 524‐526 form a pseudo‐knot and its distortion affects ribosome function. Helix 18 isolated from the ribosome context is thus an interesting fragment to investigate the structural properties and folding of RNA with pseudo‐knots.
Monika Wojciechowska, Marta Dudek, Joanna Trylska   +2 more
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Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20

Molecular Microbiology, 1993
SummaryA spontaneous mutant of Escherichia coli K‐12 was isolated that shows an increased misreading ability of all three nonsense codons together with an inability to grow at 42° C. It is demonstrated that the mutation is a deletion of the gene rpsT, coding for ribosomal protein S20. The loss of this protein not only influences the decoding properties
M, Rydén-Aulin, Z, Shaoping, P, Kylsten, L A, Isaksson   +3 more
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Directed Hydroxyl Radical Probing of 16S Ribosomal RNA in 70S Ribosomes from Internal Positions of the RNA

Biochemistry, 1998
Directed hydroxyl radical probing of 16S ribosomal RNA from Fe(II) tethered to specific sites within the RNA was used to determine RNA-RNA proximities in 70S ribosomes. We have transcribed 16S ribosomal RNA in vitro as two separate fragments, covalently attached an Fe(II) probe to a 5'-guanosine-alpha-phosphorothioate at the junction between the two ...
L F, Newcomb, H F, Noller
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[11] 16 S ribosomal RNA cataloging

1988
Publisher Summary This chapter discusses 16 S ribosomal RNA cataloging. Phylogenetic studies on cyanobacteria and chloroplasts using the 16 S ribosomal RNA (rRNA) cataloging approach as a tool have been done by two different methods: the traditional one which is generally not used anymore and the advanced technique which is presented in detail in ...
Erko Stackebrandt, Wolfgang Ludwig
openaire   +1 more source