Results 91 to 100 of about 417,138 (346)

16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene [PDF]

Journal of Clinical Microbiology, 1998
ABSTRACT Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches.
Reischl, U., Feldmann, K., Naumann, L., Gaugler, B. J., Bescher Ninet, Béatrice Alice, Hirschel, Bernard, Emler, Stefan   +6 more
openaire   +4 more sources

Photothermal Hydrogel with Mn3O4 Nanoparticles Alleviates Intervertebral Disc Degeneration by Scavenging ROS and Regulating Extracellular Matrix Metabolism

Advanced Functional Materials, EarlyView.
The MPTT‐nanozyme‐hydrogel system (Mn3O4@ChS‐HA) provides a multifunctional therapeutic strategy for intervertebral disc degeneration (IVDD), effectively targeting oxidative stress and enhancing AF repair by restoring extracellular matrix (ECM) and redox homeostasis.
Yangyang Chen, Chan Xue, Feifei Ni, Binwu Hu, Miaoheng Yan, He Zhang, Yuxiang Hu, Xiaoyao Peng, Guangfang Li, Yu Han, Hongjian Liu, Zengwu Shao, Qin Wang, Yulong Wei   +13 more
wiley   +1 more source

Deep learning for predicting 16S rRNA gene copy number

Scientific Reports
Culture-independent 16S rRNA gene metabarcoding is a commonly used method for microbiome profiling. To achieve more quantitative cell fraction estimates, it is important to account for the 16S rRNA gene copy number (hereafter 16S GCN) of different ...
Jiazheng Miao, Tianlai Chen, Mustafa Misir, Yajuan Lin   +3 more
doaj   +1 more source

rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

Microbiology Spectrum
Precise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj   +1 more source

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Satoru Watanabe, Yoichi Sakata, Keigo Mizuno, Naohiro Kishida, Naoshi Fujimoto, Masaharu Suzuki, Kenji Komatsu, Tomoki Yokoyama, Michihiro Akiba, Akihiro Ohnishi, Satoko Matsukura   +10 more
openaire   +4 more sources

Microbubble‐Controlled Delivery of Biofilm‐Targeting Nanoparticles to Treat MRSA Infection

Advanced Functional Materials, EarlyView.
Here, an effective strategy using microbubble (MB)‐controlled delivery of biofilm‐targeting nanoparticles (BTNs) for removal and therapy of methicillin‐resistant Staphylococcus aureus (MRSA) biofilm infections is introduced. In vivo delivery of MB with BTN is demonstrated to silence key bacterial genes involved in biofilm formation (icaA), bacterial ...
Ju Yeon Chung, Yujin Ahn, Joo Hun Lee, Seungju Yang, Seung Cheol Lee, Hyunjoon Kong, Hyun Jung Chung   +6 more
wiley   +1 more source

Clearance of viable Mycobacterium ulcerans from Buruli ulcer lesions during antibiotic treatment as determined by combined 16S rRNA reverse transcriptase /IS 2404 qPCR assay.

PLoS Neglected Tropical Diseases, 2017
IntroductionBuruli ulcer (BU) caused by Mycobacterium ulcerans is effectively treated with rifampicin and streptomycin for 8 weeks but some lesions take several months to heal. We have shown previously that some slowly healing lesions contain mycolactone
Mabel Sarpong-Duah, Michael Frimpong, Marcus Beissner, Malkin Saar, Ken Laing, Francisca Sarpong, Aloysius Dzigbordi Loglo, Kabiru Mohammed Abass, Margaret Frempong, Fred Stephen Sarfo, Gisela Bretzel, Mark Wansbrough-Jones, Richard Odame Phillips   +12 more
doaj   +1 more source

Colon‐Targeted Natural Polysaccharide‐Berberine Armored Hydrogel for the Treatment of Colitis

Advanced Healthcare Materials, EarlyView.
In this research, a novel hydrogel system targeting the colon is developed, incorporating Rhubarb polysaccharides and berberine‐loaded dendrimer. This hydrogel, forms through intermolecular hydrogen bonding and electrostatic interactions, accumulates in colonic tissues, effectively alleviating pathological immune hyperactivation while modulating gut ...
Miao Guo, Bo Li, Hongyi Li, Yi Chen, Qin Yuan, Mingju Shui, Hefeng Zhou, Wei Hao, Shengpeng Wang   +8 more
wiley   +1 more source

Therapeutic Implants: Mechanobiologic Enhancement of Osteogenic, Angiogenic, and Myogenic Responses in Human Mesenchymal Stem Cells on 3D‐Printed Titanium Truss

Advanced Healthcare Materials, EarlyView.
This study investigates a synergistic effect between 3D‐printed surface features and mechanical micro‐strain in enhancing the osteogenic, angiogenic, and myogenic responses of human mesenchymal stem cells (hMSCs). Load‐induced mechanotransduction, facilitated by the implant's architectural design, significantly amplifies hMSC differentiation.
Se‐Hwan Lee, Ali Kiapour, Brendan D. Stoeckl, Ellen Y. Zhang, Matthew R. Begley, Jarod Oldham, Lewis Harrison, Jessee Hunt, Robert L. Mauck, Su Chin Heo   +9 more
wiley   +1 more source

Harnessing the intragenomic variability of rRNA operons to improve differentiation of Vibrio species

Scientific Reports
Although the 16S rRNA gene is frequently used as a phylogenetic marker in analysis of environmental DNA, this marker often fails to distinguish closely related species, including those in the genus Vibrio.
Amaia Leunda-Esnaola, Evgeni Bunin, Pablo Arrufat, Peter B. Pearman, Vladimir R. Kaberdin   +4 more
doaj   +1 more source