Results 91 to 100 of about 393,637 (235)

Gut Microbiota‐Derived Anandamide Mediates the Therapeutic Effects of Urolithin A on Alcohol‐Induced Cognitive and Social Dysfunction via CB1R‐DRD2‐RAP1 Signaling Axis

Advanced Science, EarlyView.
This study reveals that Urolithin A (UA) counteracts alcohol‐induced cognitive and social dysfunction (AICSD) via a gut microbiome‐dependent mechanism. UA‐enriched Bacteroids sartorii and Parabacteroids distasonis elevate anandamide (AEA), which activates the CB1R‐DRD2‐Rap1 signaling cascade to drive synaptic repair and reduce neuroinflammation ...
Hongbo Zhang, Zibin Li, Yao Xiao, Ji Bian, Caian He, Chao Liu, Lan Gong, Lin Han, Zhigang Liu, Min Wang   +9 more
wiley   +1 more source

Multiplex-PCR protocol development for rapid screening of white spot syndrome virus (WSSV) in shrimp

Journal of Fisheries, 2020
This study was aimed to develop a faster single step multiplex PCR protocol for the simultaneous detection of white spot syndrome virus (WSSV) with its host (i.e. shrimp) as internal positive control. To do so, four combinations of primer were tested (I.
Sharmin Aktar, Md. Shahin Parvez, H M Rakibul Islam, Md. Nazmul Ahsan   +3 more
doaj  

Bioprinting of Microtissues Within Mechanically Tunable Support Baths to Engineer Anisotropic Musculoskeletal Tissues

Advanced Science, EarlyView.
This study presents a novel 4D bioprinting platform for engineering biomimetic musculoskeletal grafts. By tuning the mechanical properties of support baths, we enhance tissue fusion, collagen alignment, and cell differentiation. Using this strategy, we successfully fabricate scaled‐up, anisotropic tissues such as meniscus, articular cartilage, and ...
Francesca D. Spagnuolo, Gabriela S. Kronemberger, Daniel J. Kelly   +2 more
wiley   +1 more source

Terminal restriction fragment length polymorphism is an “old school” reliable technique for swift microbial community screening in anaerobic digestion [PDF]

, 2018
The microbial community in anaerobic digestion has been analysed through microbial fingerprinting techniques, such as terminal restriction fragment length polymorphism (TRFLP), for decades.
De Vrieze, Jo, Ijaz, Umer Z., Saunders, Aaron M., Theuerl, Susanne   +3 more
core   +6 more sources

ANALISIS SEKUENSING 16S rRNA DI BIDANG MIKROBIOLOGI

JKS (Jurnal Kedokteran Syiah Kuala), 2011
Abstrak. Gen 16S ribosomal RNA (16S rRNA) memiliki daerah yang conserved (lestari) sehingga tepat digunakan dalam Polymerase Chain Reaction (PCR) dan analisis sekuensing untuk menentukan taksonomi, filogeni dan keanekaragaman antar spesies.
Tristia Rinanda
doaj  

The overlooked evolutionary dynamics of 16S rRNA revises its role as the “gold standard” for bacterial species identification

Scientific Reports
The role of 16S rRNA has been and largely remains crucial for the identification of microbial organisms. Although 16S rRNA could certainly be described as one of the most studied sequences ever, the current view of it remains somewhat ambiguous.
Oldřich Bartoš, Martin Chmel, Iva Swierczková   +2 more
doaj   +1 more source

Biodegradable Carbonate Nanogels Loaded with Anti MFAP‐5 siRNA for Anti‐stromal Therapy of Hepatocellular Carcinoma

Advanced Science, EarlyView.
Polycarbonate nanogels enable lipid‐free delivery of anti‐MFAP‐5 siRNA into cancer‐associated fibroblasts (CAF) in hepatocellular carcinoma. In a cirrhotic murine model, this approach silences MFAP‐5, reduces fibroblast activation, and suppresses tumor growth by inhibiting NOTCH/Hes1‐driven angiogenesis. CAF‐targeted MFAP‐5 RNAi and conserved signaling
Paul Schneider, Alexander Fuchs, Michael Fichter, Vanessa Bolduan, Jenny Schunke, Özlem Akilli, Nádia C. Correia, Yannick Ehlers, Christian Czysch, Pia Winterwerber, Eliana Stanganello, Barbara Schrörs, Mustafa Diken, Christoph Eckert, Shusruto Rishik, Andreas Keller, Jörn M. Schattenberg, Stephan Grabbe, Matthias Bros, Lutz Nuhn, Leonard Kaps   +20 more
wiley   +1 more source

Bacterial Community Reconstruction Using A Single Sequencing Reaction [PDF]

, 2010
Bacteria are the unseen majority on our planet, with millions of species and comprising most of the living protoplasm. While current methods enable in-depth study of a small number of communities, a simple tool for breadth studies of bacterial population
Amir, Amnon, Zuk, Or
core  

Primer selection impacts specific population abundances but not community dynamics in a monthly time-series 16S rRNA gene amplicon analysis of coastal marine bacterioplankton. [PDF]

, 2018
Primers targeting the 16S small subunit ribosomal RNA marker gene, used to characterize bacterial and archaeal communities, have recently been re-evaluated for marine planktonic habitats.
Carlson, Craig A, Nelson, Craig E, Wear, Emma K, Wilbanks, Elizabeth G   +3 more
core   +1 more source

Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

Advanced Science, EarlyView.
In MASLD, YTHDC1 undergoes increased lactylation and ubiquitination, reducing its expression. AARS1 mediates lactylation at lysine 565, while disrupted binding to LDHA further promotes lactylation, suppressing YTHDC1. This downregulation enhances PTPN22 mRNA stability, leading to NLRP3 dephosphorylation and activation, which exacerbates inflammation ...
Feng Zhang, Linghua Zeng, Kunkun Zou, Keying Lin, Fangting Yao, Jinglun Song, Shumeng Zhang, Hanshu Feng, Zhichao Yang, Chunlei Wang, Hongtao Diao, Xue Kong, Tengfei Pan, Joonki Kim, Tianqi Duo, Linqiang Li, Yu Bian   +16 more
wiley   +1 more source