Results 101 to 110 of about 292,385 (304)

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

Multiplex-PCR protocol development for rapid screening of white spot syndrome virus (WSSV) in shrimp

Journal of Fisheries, 2020
This study was aimed to develop a faster single step multiplex PCR protocol for the simultaneous detection of white spot syndrome virus (WSSV) with its host (i.e. shrimp) as internal positive control. To do so, four combinations of primer were tested (I.
Sharmin Aktar, Md. Shahin Parvez, H M Rakibul Islam, Md. Nazmul Ahsan   +3 more
doaj  

Community analysis of picocyanobacteria in an oligotrophic lake by cloning 16S rRNA gene and 16S rRNA gene amplicon sequencing

The Journal of General and Applied Microbiology, 2015
In this study, the picocyanobacterial species composition of Lake Miyagase was examined by analyzing the 16S rRNA gene in a clone library and by amplicon sequencing using a benchtop next-generation sequencer. Five separate samples were analyzed from different days over a ten-month period. In the picocyanobacterial lineage, 9 and 12 OTUs were identified
Fujimoto, Naoshi, Mizuno, Keigo, Yokoyama, Tomoki, Ohnishi, Akihiro, Suzuki, Masaharu, Watanabe, Satoru, Komatsu, Kenji, Sakata, Yoichi, Kishida, Naohiro, Akiba, Michihiro, Matsukura, Satoko   +10 more
openaire   +3 more sources

Canagliflozin Alleviates Diabetic Glomerular Endothelial Injury via Melibiose in a Microbiota‐Dependent Manner

Advanced Science, EarlyView.
Canagliflozin treatment reshapes the gut microbiota in DKD and elevates levels of melibiose, a metabolite derived from Roseburia intestinalis. Melibiose directly binds to and enhances the enzymatic activity of glyoxalase 1, leading to decreased methylglyoxal accumulation.
Wei Zhang, Yi Song, Changkun Li, Yuanyuan Luo, Mingwei Shao, Feng Guo, Fangyi Wei, Xunjie Fan, Wenwen Guo, Fengmei Xu, Yanhong Sang, Dongming Zhang, Yanhong Zhou, Lianwei Wang, Zhiqiang Kang, Yingjun Yang, Chunhua Song, Yanxia Liu, Xiaojun Ma, Jiao Wang, Chong Li, Shengnan Ma, Lin Zhao, Zhi Qin, Guolan Xing, Qiubo Zhao, Jun Li, Shumin Song, Dan Zhao, Ting Huang, Qingzhu Wang, Yanyan Zhao, Guijun Qin   +32 more
wiley   +1 more source

Application of 16S rRNA metagenomics to analyze bacterial communities at a respiratory care centre in Taiwan

, 2017
In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed.
Tang, Chuan Yi;Yiu, Siu-Ming;Kuo, Han-Yueh;Tan, Te-Sheng;Liao, Ki-Hok;Liu, Chih-Chin;Hon, Wing-Kai;Liou, Ming-Li, 郭漢岳;郭弘揚   +1 more
core   +1 more source

ANALISIS SEKUENSING 16S rRNA DI BIDANG MIKROBIOLOGI

JKS (Jurnal Kedokteran Syiah Kuala), 2011
Abstrak. Gen 16S ribosomal RNA (16S rRNA) memiliki daerah yang conserved (lestari) sehingga tepat digunakan dalam Polymerase Chain Reaction (PCR) dan analisis sekuensing untuk menentukan taksonomi, filogeni dan keanekaragaman antar spesies.
Tristia Rinanda
doaj  

The overlooked evolutionary dynamics of 16S rRNA revises its role as the “gold standard” for bacterial species identification

Scientific Reports
The role of 16S rRNA has been and largely remains crucial for the identification of microbial organisms. Although 16S rRNA could certainly be described as one of the most studied sequences ever, the current view of it remains somewhat ambiguous.
Oldřich Bartoš, Martin Chmel, Iva Swierczková   +2 more
doaj   +1 more source

Multi‐Enzyme Mimetic Molybdenum Nitride Nanozymes Reshape Subgingival Microenvironment for Synergistic Periodontitis Therapy via ROS Regulation and Microbiome Remodeling

Advanced Science, EarlyView.
ABSTRACT Periodontitis, a chronic inflammatory disease initiated and sustained by plaque microorganisms and host immune response, remains an intractable oral disease and a leading cause of tooth loss worldwide. Traditional mechanical debridement and adjunctive antibiotic or antiseptic therapy often shows limited efficacy due to the complex anatomical ...
Weiyu Zhang, Zhongwei Yang, Yunfan Zhang, Longwei Wang, Xiaoyu Zhang, Jiahui Mao, Yizhi Dai, Yifan Yuan, Mingge Wang, Xin Yang, Xin Yu, Jing Liu, Chunying Chen   +12 more
wiley   +1 more source

Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota‐Mediated Bile Acid Homeostasis

Advanced Science, EarlyView.
Nanocellulose, derived from microcrystalline cellulose (MCC) through sulfuric acid hydrolysis or mechanical grinding to produce CNC or CNF, was tested in a rat ICP model. Particularly, CNF improved gut microbiota composition, reduced secondary bile acid metabolism, and restored bile acid homeostasis through modulation of the gut–liver axis.
Muhua Yu, Hongjie Dai, Xiaocui Zhong, Qibin Li, Hui Yuan, Yang Yang, Daiyong Huang, Lei Zhang, Rui Ran, Tian He, Yuanzhi Huang, Silas Villas‐Boas, Sergey Tumanov, Richard D. Cannon, Boris Novakovic, Richard Saffery, Yuhao Zhang, Xiaojing Dong, Ting‐Li Han   +18 more
wiley   +1 more source

High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota

, 2008
The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU ...
Vos, W.M., de, de Vos, Willem M., Zoetendal, Erwin G., Rajilic-Stojanovic, M., Zoetendal, E.G., Rajilić-Stojanović, Mirjana, de Vos, W.M.   +6 more
core   +1 more source