Results 81 to 90 of about 410,225 (302)
Deep learning for predicting 16S rRNA gene copy number
Scientific ReportsCulture-independent 16S rRNA gene metabarcoding is a commonly used method for microbiome profiling. To achieve more quantitative cell fraction estimates, it is important to account for the 16S rRNA gene copy number (hereafter 16S GCN) of different ...
Jiazheng Miao +3 more
doaj +1 more source
Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis
Emerging Infectious Diseases, 2002 In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi +7 more
doaj +1 more source
High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota [PDF]
, 2008 The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU ...
Vos, W.M., de +6 more
core +1 more source
Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription
FEBS Open Bio, EarlyView.In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang +10 more
wiley +1 more source
Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model
FEBS Open Bio, EarlyView.This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito +7 more
wiley +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Harnessing the intragenomic variability of rRNA operons to improve differentiation of Vibrio species
Scientific ReportsAlthough the 16S rRNA gene is frequently used as a phylogenetic marker in analysis of environmental DNA, this marker often fails to distinguish closely related species, including those in the genus Vibrio.
Amaia Leunda-Esnaola +4 more
doaj +1 more source
Microbiology SpectrumPrecise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj +1 more source
How conserved are the conserved 16S-rRNA regions? [PDF]
PeerJ, 2017 The 16S rRNA gene has been used as master key for studying prokaryotic diversity in almost every environment. Despite the claim of several researchers to have the best universal primers, the reality is that no primer has been demonstrated to be truly ...
Marcel Martinez-Porchas +3 more
doaj +2 more sources