Results 101 to 110 of about 417,138 (346)

Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

mSystems, 2015
We continue to uncover a wealth of information connecting microbes in important ways to human and environmental ecology. As our scientific knowledge and technical abilities improve, the tools used for microbiome surveys can be modified to improve the ...
William Walters, Embriette R. Hyde, Donna berg-Lyons, Gail L Ackermann, G. Humphrey, A. Parada, J. Gilbert, J. Jansson, J. Caporaso, J. Fuhrman, A. Apprill, R. Knight   +11 more
semanticscholar   +1 more source

Suppressing t(4;11) Acute Leukemia by Lipopolymer Nanoparticle Delivery of siRNA Targeting KMT2A::AFF1 with Enhanced Extrahepatic Delivery

Advanced Healthcare Materials, EarlyView.
This study introduces a new lipopolymer nanoparticle (LPNP) system that efficiently delivers siRNA to leukemia cells. The LPNPs silence the leukemia fusion gene KMT2A::AFF1, induce apoptosis, and decrease leukemia burden in mice. These results demonstrate the potential of LPNPs as a targeted siRNA therapy for acute lymphoblastic leukemia.
Mohammad Nasrullah, Remant KC, Amarnath Praphakar Rajendran, Saba Abbasi Dezfouli, Cezary Kucharski, Xiaoyan Jiang, Spencer B. Gibson, Joseph Brandwein, Olaf Heidenreich, Hasan Uludağ   +9 more
wiley   +1 more source

16S rRNA methyltransferases: the end of aminoglycosides?

, 2019
16S rRNA methyltransferases (16S RMTases) confer high-level aminoglycoside resistance (MICs >256 mg/L) to Gram-negative bacteria and are an emerging threat. The prevalence of 16S RMTases in the UK is entirely unknown as are the drivers behind their emergence.
openaire   +4 more sources

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source

Accuracy of taxonomy prediction for 16S rRNA and fungal ITS sequences

PeerJ, 2018
Prediction of taxonomy for marker gene sequences such as 16S ribosomal RNA (rRNA) is a fundamental task in microbiology. Most experimentally observed sequences are diverged from reference sequences of authoritatively named organisms, creating a challenge
Robert C. Edgar
semanticscholar   +1 more source

Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard

MicrobiologyOpen, 2020
Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here, we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental ...
O. Zemb, C. Achard, J. Hamelin, M. De Almeida, B. Gabinaud, L. Cauquil, L. Verschuren, J. Godon   +7 more
semanticscholar   +1 more source

Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing

PLoS ONE, 2013
Tuberculosis (TB) remains a global threat in the 21st century. Traditional studies of the disease are focused on the single pathogen Mycobacterium tuberculosis. Recent studies have revealed associations of some diseases with an imbalance in the microbial community.
Man Kit Cheung, Stephen Kwok-Wing Tsui, Patrick Tik Wan Law, Hoi Shan Kwan, Wai Yip Lam, Kai Man Kam, Wenyan Nong, Chun Hang Au, Wendy Yin Wan Fung   +8 more
openaire   +5 more sources

From Lab to Market: Paper‐Based CRISPR Diagnostics and Commercialization Pathways

Advanced Sensor Research, EarlyView.
Paper‐based CRISPR diagnostics represents one of the most widely commercialized CRISPR assay technologies, yet challenges remain for broader point‐of‐care use. Abstract The global health landscape is continually challenged by infectious diseases that can swiftly escalate into pandemics, underscoring the need for rapid, accurate, and cost‐effective ...
Selen Dalgan, Qingshan Wei
wiley   +1 more source

NOX2 Contributes to High‐Frequency Outer Hair Cell Vulnerability in the Cochlea

Advanced Science, EarlyView.
This study first identifies NOX2 as a differentially expressed gene related to oxidative damage in the apical and basal turns through single‐cell RNA sequencing. NOX2 gene knockout mitigates OHCs damage caused by neomycin and noise and enhances Nrf2 expression and nuclear translocation.
Meihao Qi, Zejun Gao, Yang Qiu, Renfeng Wang, Keyong Tian, Bo Yue, Xinyu Zhang, Peng Zhang, Ziqi Wu, Qingwen Zhu, Zhenzhen Liu, Zhuoyao Ma, Xueying Zhou, Yu Han, Jun Chen, Jianhua Qiu, DingJun Zha   +16 more
wiley   +1 more source

Multiplex-PCR protocol development for rapid screening of white spot syndrome virus (WSSV) in shrimp

Journal of Fisheries, 2020
This study was aimed to develop a faster single step multiplex PCR protocol for the simultaneous detection of white spot syndrome virus (WSSV) with its host (i.e. shrimp) as internal positive control. To do so, four combinations of primer were tested (I.
Sharmin Aktar, Md. Shahin Parvez, H M Rakibul Islam, Md. Nazmul Ahsan   +3 more
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