Results 71 to 80 of about 393,637 (235)

Deep learning for predicting 16S rRNA gene copy number

Scientific Reports
Culture-independent 16S rRNA gene metabarcoding is a commonly used method for microbiome profiling. To achieve more quantitative cell fraction estimates, it is important to account for the 16S rRNA gene copy number (hereafter 16S GCN) of different ...
Jiazheng Miao, Tianlai Chen, Mustafa Misir, Yajuan Lin   +3 more
doaj   +1 more source

How conserved are the conserved 16S-rRNA regions? [PDF]

PeerJ, 2017
The 16S rRNA gene has been used as master key for studying prokaryotic diversity in almost every environment. Despite the claim of several researchers to have the best universal primers, the reality is that no primer has been demonstrated to be truly ...
Marcel Martinez-Porchas, Enrique Villalpando-Canchola, Luis Enrique Ortiz Suarez, Francisco Vargas-Albores   +3 more
doaj   +2 more sources

Efficacy and Safety Assessment of 5‐Fluorouracil, Irinotecan and Oxaliplatin‐Loaded Implants in Mouse and Pig Models for Pancreatic Cancer Therapy

Advanced Materials, EarlyView.
A novel FIRINOX‐loaded implant demonstrates controlled drug release, and potent therapeutic efficacy in pancreatic ductal adenocarcinoma mouse models with safety confirmed in a large‐animal pre‐clinical model. This innovative drug delivery platform offers a promising new treatment approach for non‐resectable, treatment‐resistant pancreatic cancer ...
Samantha J. Wade, Ashna A. Kumar, Samuel O. Nitschke, Paul Joyce, Elahe Minaei, Stephanie E. Reuter Lange, Chelsea Penney, Holly Warren, Ruby Shepherd, Jeremy N. Dinoro, Gordon G. Wallace, Anthony Dosseto, Sameera Ansar, Naila Islam, Alistair Lochhead, George Sharbeen, Phoebe A. Phillips, Morteza Aghmesheh, Wayne J. Hawthorne, Kara L. Vine‐Perrow   +19 more
wiley   +1 more source

Identification of Host-Specific Bacteroidales 16S rDNA Sequences from Human Sewage and Ruminant Feces [PDF]

, 2011
The need to identify the source of fecal contamination of water has led to the development of various fecal source identification methods, a field known as microbial source tracking (MST). One promising method of MST focuses on fecal members of the order
Allsop, Ashelford, Ashelford, Bernhard, Dick, Dorai-Raj, Dore, Fiksdal, Hayashi, Hutchison, Kildare, Kobayashi, Kreader, Lamendella, Leven, Maidak, Mieszkin, Okabe, Ozutsumi, Paster, Rajendhran, Suau, Tajima, von Wintzingerode, Wery, Whitford   +25 more
core   +1 more source

rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

Microbiology Spectrum
Precise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj   +1 more source

High‐Throughput 3D Matrigel‐based Droplet Microfluidics for Single‐Cell Function‐to‐Omics Analysis of Cytotoxic Immune Cells in Solid Tumor Interactions

Advanced Materials Technologies, EarlyView.
A 3D Matrigel‐based droplet microfluidics platform that allows physiologically relevant analysis of single immune‐tumor interactions in solid tumors. By integrating real‐time functional imaging with fluorescence‐activated droplet sorting and RNA sequencing, the system links immune cytotoxic behavior to transcriptomic profiles, providing a scalable ...
Christina Sharkey, Harry Akligoh, Michael Finocchiaro, Abhishek Srihari Rampelli, Zongwei Wang, Aria F. Olumi, Tania Konry   +6 more
wiley   +1 more source

Microbial residence time is a controlling parameter of the taxonomic composition and functional profile of microbial communities. [PDF]

, 2019
A remaining challenge within microbial ecology is to understand the determinants of richness and diversity observed in environmental microbial communities.
Achermann, Stefan, Fenner, Kathrin, Johnson, David R, Joss, Adriano, Mansfeldt, Cresten, Men, Yujie, Villez, Kris, Walser, Jean-Claude   +7 more
core   +3 more sources

Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]

, 2020
Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet, Gryp, Tessa, Joossens, M., Vaneechoutte, Mario   +3 more
core   +1 more source

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source

Harnessing the intragenomic variability of rRNA operons to improve differentiation of Vibrio species

Scientific Reports
Although the 16S rRNA gene is frequently used as a phylogenetic marker in analysis of environmental DNA, this marker often fails to distinguish closely related species, including those in the genus Vibrio.
Amaia Leunda-Esnaola, Evgeni Bunin, Pablo Arrufat, Peter B. Pearman, Vladimir R. Kaberdin   +4 more
doaj   +1 more source