Results 81 to 90 of about 403,409 (349)

Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

Emerging Infectious Diseases, 2002
In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these ...
Claudio T. Sacchi, Anne M. Whitney, Leonard W. Mayer, Roger Morey, Arnold Steigerwalt, Ariana Boras, Robin S. Weyant, Tanja Popovic   +7 more
doaj   +1 more source

New Insights into Atonic Postpartum Hemorrhage: Animal Model Construction Based on Placental Nanodelivery Systems

Advanced Healthcare Materials, EarlyView.
This study develops a placenta‐targeted nanodelivery system co‐loading HMGB1 protein and the NLRP3 agonist nigericin to establish an animal model of atonic postpartum hemorrhage. The model accurately recapitulates clinical phenotypes, including prolonged labor and uterine contractility dysfunction, while revealing inflammatory activation in placental ...
Jiangxue Qu, Ruiqi Wu, Hai Jiang, Lian Chen, Yingying Tian, Shuai Zeng, Tongmin Liu, Cheng Zhao, Huifeng Shi, Xiaolong Liang, Yangyu Zhao   +10 more
wiley   +1 more source

rRNA operon improves species-level classification of bacteria and microbial community analysis compared to 16S rRNA

Microbiology Spectrum
Precise identification of species is fundamental in microbial genomics and is crucial for understanding the microbial communities. While the 16S rRNA gene, particularly its V3-V4 regions, has been extensively employed for microbial identification ...
Sohyoung Won, Seoae Cho, Heebal Kim
doaj   +1 more source

Targeting the ARRDC3–DRP1 Axis via hUMSC‐Derived Exosomal CRYAB for Neuroprotection in Cerebral Ischemia/Reperfusion Injury

Advanced Healthcare Materials, EarlyView.
Intranasally administered hUMSC‐derived exosomes modulate the CRYAB–ARRDC3–Drp1 axis, alleviating mitochondrial dysfunction and ferroptosis, enhancing neuronal survival, reducing oxidative stress, and promoting functional recovery in ischemia‐reperfusion injury, offering a promising therapeutic strategy for ischemic stroke.
Rong ji, Zengyu Zhang, Zhuohang Liu, Kaicheng Yang, Xueyu Mao, Min Chu, Yong Wang, Jing Zhao   +7 more
wiley   +1 more source

Harnessing the intragenomic variability of rRNA operons to improve differentiation of Vibrio species

Scientific Reports
Although the 16S rRNA gene is frequently used as a phylogenetic marker in analysis of environmental DNA, this marker often fails to distinguish closely related species, including those in the genus Vibrio.
Amaia Leunda-Esnaola, Evgeni Bunin, Pablo Arrufat, Peter B. Pearman, Vladimir R. Kaberdin   +4 more
doaj   +1 more source

Clearance of viable Mycobacterium ulcerans from Buruli ulcer lesions during antibiotic treatment as determined by combined 16S rRNA reverse transcriptase /IS 2404 qPCR assay.

PLoS Neglected Tropical Diseases, 2017
IntroductionBuruli ulcer (BU) caused by Mycobacterium ulcerans is effectively treated with rifampicin and streptomycin for 8 weeks but some lesions take several months to heal. We have shown previously that some slowly healing lesions contain mycolactone
Mabel Sarpong-Duah, Michael Frimpong, Marcus Beissner, Malkin Saar, Ken Laing, Francisca Sarpong, Aloysius Dzigbordi Loglo, Kabiru Mohammed Abass, Margaret Frempong, Fred Stephen Sarfo, Gisela Bretzel, Mark Wansbrough-Jones, Richard Odame Phillips   +12 more
doaj   +1 more source

Bacterial Community Reconstruction Using A Single Sequencing Reaction [PDF]

, 2010
Bacteria are the unseen majority on our planet, with millions of species and comprising most of the living protoplasm. While current methods enable in-depth study of a small number of communities, a simple tool for breadth studies of bacterial population
Amir, Amnon, Zuk, Or
core  

Geobacter, Anaeromyxobacter and Anaerolineae populations are enriched on anodes of root exudate-driven microbial fuel cells in rice field soil [PDF]

, 2015
Plant-based sediment microbial fuel cells (PMFCs) couple the oxidation of root exudates in living rice plants to current production. We analysed the composition of the microbial community on anodes from PMFC with natural rice field soil as substratum for
Arends, Bond, Bond, Chae, Cheng, Chung, Conrad, Davis, De Schamphelaire, Donovan, Holmes, Holmes, Hori, Ishii, Ishii, Jung, Kaku, Kramer, Lee, Liu, Logan, Lovley, Lueders, Lueders, Mengoni, Rabaey, Reimers, Richter, Strik, Sun, Tender, Timmers, Treude, Yamada   +33 more
core   +1 more source

3D‐Printed Gastrointestinal Stents: In Vivo Evaluation in a Swine Small Bowel Perforation Model

Advanced Healthcare Materials, EarlyView.
Gastrointestinal fistulae and perforations can lead to severe complications including sepsis and patient death. In this work, the efficacy of 3D‐printed gastrointestinal stents composed of poly‐lactic‐acid (PLA) was evaluated in an in vivo swine model.
Gweniviere Capron, Parinaz Fathi, Tor Wolf Jensen, Derek J. Milner, André J. van der Vlies, Regan L. Moody, Elizabeth A. Bangert, Blair Rowitz, Dipanjan Pan, Matthew B. Wheeler   +9 more
wiley   +1 more source

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

Advanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang, Houyu Ling, Xizong Chen, Yufei Duan, Yu Liu, Shuntian Cai, Yandan Ren, Wenlong Li, Lei Ren, Miao Wang   +9 more
wiley   +1 more source