Results 41 to 50 of about 246,967 (290)

Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]

, 2011
Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Bogert, B., van den, Vos, W.M., de, Zoetendal, E.G., Kleerebezem, M.   +3 more
core   +1 more source

16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice [PDF]

, 2015
Acknowledgements The authors acknowledge the assistance of Grietje Holtrop (RINH-BioSS) with the statistical analysis of the data and the Wellcome Trust Sanger Institute’s 454 pyrosequencing team for generating 16S rRNA gene data. AWW, PS and JP received
Flint, Harry J., Martin, Jennifer C., Parkhill, Julian, Scott, Karen P., Scott, Paul, Walker, Alan W.   +5 more
core   +2 more sources

Small and mighty: adaptation of superphylum Patescibacteria to groundwater environment drives their genome simplicity. [PDF]

, 2020
BackgroundThe newly defined superphylum Patescibacteria such as Parcubacteria (OD1) and Microgenomates (OP11) has been found to be prevalent in groundwater, sediment, lake, and other aquifer environments.
Adams, Benjamin G, Alm, Eric J, Arkin, Adam P, Detienne, Brittny L, Fields, Matthew W, Gao, Shuhong, Hazen, Terry C, He, Zhili, Joyner, Dominique C, Klingeman, Dawn M, Lowe, Kenneth A, Ning, Daliang, Rocha, Andrea M, Shi, Weiling, Spencer, Sarah J, Stahl, David A, Tian, Renmao, Wu, Linwei, Yang, Yunfeng, Zhang, Ping, Zhang, Ya, Zhou, Jizhong   +21 more
core   +2 more sources

Catheter-Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing [PDF]

Journal of Clinical Microbiology, 2002
ABSTRACT We describe the application of 16S rRNA gene sequencing in defining two cases of catheter-related Microbacterium bacteremia. In the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia.
Yuen, KY, Lau, SKP, Woo, PCY, Woo, GKS
openaire   +4 more sources

Information about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification.

PLoS ONE, 2019
Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, its use remains limited due to the presence of variable copy numbers with sequence variation ...
Jerald Conrad Ibal, Huy Quang Pham, Chang Eon Park, Jae-Ho Shin   +3 more
doaj   +1 more source

Novel hemotropic mycoplasmas are widespread and genetically diverse in vampire bats [PDF]

, 2017
Bats (Order: Chiroptera) have been widely studied as reservoir hosts for viruses of concern for human and animal health. However, whether bats are equally competent hosts of non-viral pathogens such as bacteria remains an important open question. Here,
Altizer, S.M., Becker, D.J., Bergner, L.M., Camus, M.S., Chizhikov, V.E., Orton, R.J., Streicker, D.G., Volokhov, D.V.   +7 more
core   +1 more source

Accurate Identification of Common Pathogenic Nocardia Species: Evaluation of a Multilocus Sequence Analysis Platform and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. [PDF]

PLoS ONE, 2016
Species identification of Nocardia is not straightforward due to rapidly evolving taxonomy, insufficient discriminatory power of conventional phenotypic methods and also of single gene locus analysis including 16S rRNA gene sequencing.
Meng Xiao, Lu Pang, Sharon C-A Chen, Xin Fan, Li Zhang, Hai-Xia Li, Xin Hou, Jing-Wei Cheng, Fanrong Kong, Yu-Pei Zhao, Ying-Chun Xu   +10 more
doaj   +1 more source

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco) [PDF]

, 2012
To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns.
Amar, Mohamed, Bendaou, Najib, Berrada, Imane, De Vos, Paul, El Fahime, ElMostafa, Melloul, Marouane, Swings, Jean, Willems, Anne   +7 more
core   +1 more source

Comparing the Efficacy of MALDI-TOF MS and Sequencing-Based Identification Techniques (Sanger and NGS) to Monitor the Microbial Community of Irrigation Water

Microorganisms, 2023
In order to intensify and guarantee the agricultural productivity and thereby to be able to feed the world’s rapidly growing population, irrigation has become very important. In parallel, the limited water resources lead to an increase in usage of poorly
Botond Bendegúz Surányi, Benjamin Zwirzitz, Csilla Mohácsi-Farkas, Tekla Engelhardt, Konrad J. Domig   +4 more
doaj   +1 more source

Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules. [PDF]

PLoS ONE, 2016
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes.
James E McDonald, Niels Larsen, Andrea Pennington, John Connolly, Corrin Wallis, David J Rooks, Neil Hall, Alan J McCarthy, Heather E Allison   +8 more
doaj   +1 more source