Results 41 to 50 of about 253,664 (304)

Information about variations in multiple copies of bacterial 16S rRNA genes may aid in species identification.

PLoS ONE, 2019
Variable region analysis of 16S rRNA gene sequences is the most common tool in bacterial taxonomic studies. Although used for distinguishing bacterial species, its use remains limited due to the presence of variable copy numbers with sequence variation ...
Jerald Conrad Ibal, Huy Quang Pham, Chang Eon Park, Jae-Ho Shin   +3 more
doaj   +1 more source

Comparing the Efficacy of MALDI-TOF MS and Sequencing-Based Identification Techniques (Sanger and NGS) to Monitor the Microbial Community of Irrigation Water

Microorganisms, 2023
In order to intensify and guarantee the agricultural productivity and thereby to be able to feed the world’s rapidly growing population, irrigation has become very important. In parallel, the limited water resources lead to an increase in usage of poorly
Botond Bendegúz Surányi, Benjamin Zwirzitz, Csilla Mohácsi-Farkas, Tekla Engelhardt, Konrad J. Domig   +4 more
doaj   +1 more source

Accurate Identification of Common Pathogenic Nocardia Species: Evaluation of a Multilocus Sequence Analysis Platform and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. [PDF]

PLoS ONE, 2016
Species identification of Nocardia is not straightforward due to rapidly evolving taxonomy, insufficient discriminatory power of conventional phenotypic methods and also of single gene locus analysis including 16S rRNA gene sequencing.
Meng Xiao, Lu Pang, Sharon C-A Chen, Xin Fan, Li Zhang, Hai-Xia Li, Xin Hou, Jing-Wei Cheng, Fanrong Kong, Yu-Pei Zhao, Ying-Chun Xu   +10 more
doaj   +1 more source

Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules. [PDF]

PLoS ONE, 2016
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes.
James E McDonald, Niels Larsen, Andrea Pennington, John Connolly, Corrin Wallis, David J Rooks, Neil Hall, Alan J McCarthy, Heather E Allison   +8 more
doaj   +1 more source

metaSPARSim: a 16S rRNA gene sequencing count data simulator

BMC Bioinformatics, 2019
Background In the last few years, 16S rRNA gene sequencing (16S rDNA-seq) has seen a surprisingly rapid increase in election rate as a methodology to perform microbial community studies.
Ilaria Patuzzi, Giacomo Baruzzo, Carmen Losasso, Antonia Ricci, Barbara Di Camillo   +4 more
doaj   +1 more source

SituSeq: an offline protocol for rapid and remote Nanopore 16S rRNA amplicon sequence analysis

ISME Communications, 2023
Microbiome analysis through 16S rRNA gene sequencing is a crucial tool for understanding the microbial ecology of any habitat or ecosystem. However, workflows require large equipment, stable internet, and extensive computing power such that most of the ...
Jackie Zorz, Carmen Li, Anirban Chakraborty, Daniel A. Gittins, Taylor Surcon, Natasha Morrison, Robbie Bennett, Adam MacDonald, Casey R. J. Hubert   +8 more
doaj   +1 more source

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

BMC Microbiology, 2016
Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community.
F. Fouhy, Adam G. Clooney, C. Stanton, M. Claesson, P. Cotter   +4 more
semanticscholar   +1 more source

Linking 16S rRNA Gene Classification to amoA Gene Taxonomy Reveals Environmental Distribution of Ammonia-Oxidizing Archaeal Clades in Peatland Soils

mSystems, 2021
A highly resolved taxonomy for ammonia-oxidizing archaea (AOA) based on the alpha subunit of ammonia monooxygenase (amoA) was recently established, which uncovered novel environmental patterns of AOA, challenging previous generalizations.
Haitao Wang, Alexandre Bagnoud, Rafael I. Ponce-Toledo, Melina Kerou, Micha Weil, Christa Schleper, Tim Urich   +6 more
doaj   +1 more source

The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool

Microbiome, 2018
Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion.
J. de Vrieze, A. Pinto, W. Sloan, U. Ijaz   +3 more
semanticscholar   +1 more source

Do we need a standardized 16S rRNA gene amplicon sequencing analysis protocol for poultry microbiota research?

Poultry Science
Bacteria are the major component of poultry gastrointestinal tract (GIT) microbiota and play an important role in host health, nutrition, physiology regulation, intestinal development, and growth.
Joshua M. Lyte, Mitiku M. Seyoum, Diana Ayala, Jannigje G. Kers, Valentina Caputi, Timothy Johnson, Li Zhang, Joshua Rehberger, Guolong Zhang, Sami Dridi, Brett Hale, Jean E. De Oliveira, Daniel Grum, Alexandra H. Smith, Michael Kogut, Steven C. Ricke, Anne Ballou, Bill Potter, Monika Proszkowiec-Weglarz   +18 more
doaj   +1 more source