Results 151 to 160 of about 158,818 (264)

Surveying Biodiverse Coral Reef Ecosystems Across the Tree of Life With Environmental DNA. [PDF]

Mol Ecol Resour
Wishingrad V, Bowen BW, Toonen RJ.
europepmc   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Differences in accumulation of wt 18S and miscleaved 18S rRNAs are due to differences in decay not transcription.

(A) Pulse-chase analysis of rRNA stability. Tagged wt 18S or miscleaved 18S-2 and 18S-4 rRNAs were expressed in the background of endogenous wt ribosomes (BY4741 cells).
Elise S. Brunk (18447335), Adam J. Getzler (18447338), Katrin Karbstein (232578), Melissa D. Parker (8124692)   +3 more
core   +1 more source

Identifying an appropriate endogenous control for real-time PCR of microRNAs in colorectal liver metastases: A comparison of 18s rRNA and small nuclear RNA RNU6B. [PDF]

Oncol Lett
Franz C, Hartmann S, Ulrich A, Polychronidis G, Kahlert C, Klupp F, Al-Saeedi M.   +6 more
europepmc   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Getting to the Core of the Matter-Assessing the Role of Replication in Metabarcoding-Based sedaDNA. [PDF]

Mol Ecol Resour
Baños E, Segura CR, De Boer EJ, Cundy AB, Barrera XT, Nogué S, Holman LE, Rius M.   +7 more
europepmc   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source

Bypassing Rio1 stabilizes miscleaved 18S rRNAs.

(A) 3′-RACE-sequencing of 18S rRNAs extracted from 40S ribosomal subunits purified from Gal::Pno1; Gal::Dim1 cells depleted of endogenous Pno1 and Dim1 by growth in glucose and supplemented with plasmids expressing Dim1-E85A and either Pno1 or Pno1-KKKF (
Elise S. Brunk (18447335), Adam J. Getzler (18447338), Katrin Karbstein (232578), Melissa D. Parker (8124692)   +3 more
core   +1 more source

Hybridization capture increases on-target nanopore sequencing of plant RNA tobamovirus- derived cDNA libraries. [PDF]

Front Microbiol
Espindola AS, Mosquera-Yuqui F, Ochoa-Corona F, Marek S.   +3 more
europepmc   +1 more source

Prospecting the protein design landscape

FEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona, Katharina T. Stonig, Jens Meiler, Clara T. Schoeder, Monica L. Fernández‐Quintero   +4 more
wiley   +1 more source