Structural and functional analysis of Utp24, an endonuclease for processing 18S ribosomal RNA. [PDF]
PLoS ONE, 2018 The precursor ribosomal RNA is processed by multiple steps of nucleolytic cleavage to generate mature rRNAs. Utp24 is a PIN domain endonuclease in the early 90S precursor of small ribosomal subunit and is proposed to cleave at sites A1 and A2 of pre-rRNA.
Weidong An, Yifei Du, Keqiong Ye
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Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis. [PDF]
PLoS ONE, 2014 Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems.
Yong Wang +4 more
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18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA. [PDF]
Biomed Res Int, 2016 The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis.
Leonard CA, Meli ML, Novacco M, Borel N.
europepmc +5 more sources
Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress [PDF]
BMC GenomicsBackground Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels.
Ping Han +4 more
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18S Ribosomal RNA Detection on Northern Blot Employing a Specific Oligonucleotide
BioTechniques, 2001 Elisabeth Deindl
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18S Ribosomal RNA and Tetrapod Phylogeny [PDF]
Systematic Biology, 2003 Previous phylogenetic analyses of tetrapod 18S ribosomal RNA (rRNA) sequences support the grouping of birds with mammals, whereas other molecular data, and morphological and paleontological data favor the grouping of birds with crocodiles. The 18S rRNA gene has consequently been considered odd, serving as "definitive evidence of different genes ...
Karl M. Kjer +3 more
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Structure of 18 S Ribosomal RNA in Native 40 S Ribosomal Subunits [PDF]
Journal of Biological Chemistry, 1997 We have analyzed the structure of 18 S rRNA in native 40 S subunits using chemical modification followed by primer extension. The native subunits were modified using the single-stranded specific reagents dimethyl sulfate and 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate.
Lovisa Holmberg +2 more
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Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
PLoS ONE, 2014 To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
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The final step of 40S ribosomal subunit maturation is controlled by a dual key lock
eLife, 2021 Preventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3′ end, is ...
Laura Plassart +12 more
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Basepairing with 18S ribosomal RNA in internal initiation of translation [PDF]
FEBS Letters, 1994 In concert with the translation initiation factors ‘trans‐acting’ factors function specifically during internal initiation on picornaviral mRNAs. Of these trans‐acting factors, two have been identified as the La‐protein and the polypyrimidine tract binding protein.
Gert C. Scheper +2 more
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