Results 111 to 120 of about 587,426 (318)
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj +1 more source
In Situ Detection of Aspergillus 18S Ribosomal RNA in Invasive Pulmonary Apergillosis.
We attempted to evaluate the usefulness of in situ hybridization (ISH) in the specific diagnosis of Aspergillus pulmonary infection.We used an ISH technique using a multiple digoxigenin-incorporating probe, which was constructed by means of the polymerase chain reaction (PCR) from the 18S ribosomal RNA of Aspergillus fumigatus.We studied twelve ...
Yuji Ohtsuki+5 more
openaire +4 more sources
The mechanism map of how L.johnsonii generates cyclo(pro‐trp) to alleviate CKD–SHPT by promoting the expression of CaSR and TRPV4 in IECs, enhancing Ca2+ absorption, and negatively feedback inhibiting iPTH secretion. Abstract Patients with chronic kidney disease (CKD) are at a high risk of developing secondary hyperparathyroidism (SHPT), which may ...
Xiong Zeng+13 more
wiley +1 more source
The research findings indicate that dimethyl itaconate (DI) alleviates Escherichia coli (E. coli) induced endometritis by modulating the gut microbiota and enriching the abundance of norank_f_Muribaculaceae, thereby increasing the production of guanosine.
Yuhang He+6 more
wiley +1 more source
The simplest possible beginning of abiogenesis has been a riddle from the last century, which is most successfully solved by the Lipid World hypothesis. However, origin of the next stages of evolution starting form lipids is still in dark. We propose a 'Lipid-RNA World Scenario' based on the assumption that modern stable lipid-RNA interactions are ...
arxiv
Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere
Stephen J. Taerum+3 more
doaj +1 more source
Mitofusin 1 Drives Preimplantation Development by Enhancing Chromatin Incorporation of Histone H3.3
MFN1 exerts its function partially through histone H3.3 in early embryos. MFN1 colocalizes with PADI6, and its deficiency results in compromised cytoplasmic lattices and ribosomal subunits, leading to declined H3.3 protein level. This, in turn, hinders the incorporation of H3.3 into the embryonic genome, thereby causing failure of male pronucleus ...
Xiao‐yan Shi+9 more
wiley +1 more source
RAB3B Dictates mTORC1/S6 Signaling in Chordoma and Predicts Response to mTORC1‐Targeted Therapy
RAB3B is unveiled as a prominent oncogenic regulator in chordoma, which can block the DUSP12‐mediated dephosphorylation of p‐S6 (S235/236). The combination of RAB3B and p‐S6 indicates a good prognostic value and predicts mTORC1 inhibitors response for chordoma patients.
Jianxuan Gao+15 more
wiley +1 more source
Summary: Many positive-strand RNA viruses, including all known coronaviruses, employ programmed −1 ribosomal frameshifting (−1 PRF) to regulate the translation of polycistronic viral RNAs.
Lian-Huan Wei, Yu Sun, Junjie U. Guo
doaj
Phenotypic plasticity in the Caribbean sponge Callyspongia vaginalis (Porifera: Haplosclerida)
Sponge morphological plasticity has been a long-standing source of taxonomic difficulty. In the Caribbean, several morphotypes of the sponge Callyspongia vaginalis have been observed.
Susanna López-Legentil+4 more
doaj +1 more source