18S Ribosomal RNA Evaluation as Preanalytical Quality Control for Animal DNA. [PDF]
The 18S ribosomal RNA (rRNA) gene is present in all eukaryotic cells. In this study, we evaluated the use of this gene to verify the presence of PCR-amplifiable host (animal) DNA as an indicator of sufficient sample quality for quantitative real-time PCR (qPCR) analysis.
Leonard CA, Meli ML, Novacco M, Borel N.
europepmc +5 more sources
18S Ribosomal RNA Detection on Northern Blot Employing a Specific Oligonucleotide [PDF]
Elisabeth Deindl
doaj +4 more sources
Haematobia irritans clone Hi-18S-ab-fr 18S ribosomal RNA gene, partial sequence [PDF]
GenBank: EU013947.1 Haematobia irritans clone Hi-18S-ab-fr 18S ribosomal RNA gene, partial sequenceFil: Sonvico, A.. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Filiberti, Adrian.
Filiberti, Adrian +2 more
core +2 more sources
Taxonomic profiling of individual nematodes isolated from copse soils using deep amplicon sequencing of four distinct regions of the 18S ribosomal RNA gene. [PDF]
Kenmotsu H, Uchida K, Hirose Y, Eki T.
europepmc +3 more sources
Basepairing with 18S ribosomal RNA in internal initiation of translation [PDF]
In concert with the translation initiation factors ‘trans‐acting’ factors function specifically during internal initiation on picornaviral mRNAs. Of these trans‐acting factors, two have been identified as the La‐protein and the polypyrimidine tract binding protein.
Gert C. Scheper +2 more
openalex +5 more sources
The 3'-5' exoribonuclease ISG20L2 contributes to 3' terminus maturation of 18S and 28S ribosomal RNAs. [PDF]
Aubert M +9 more
europepmc +2 more sources
In Situ Detection of Aspergillus 18S Ribosomal RNA in Invasive Pulmonary Apergillosis.
We attempted to evaluate the usefulness of in situ hybridization (ISH) in the specific diagnosis of Aspergillus pulmonary infection.We used an ISH technique using a multiple digoxigenin-incorporating probe, which was constructed by means of the polymerase chain reaction (PCR) from the 18S ribosomal RNA of Aspergillus fumigatus.We studied twelve ...
Makoto Kobayashi +5 more
openalex +4 more sources
Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
doaj +1 more source
18S Ribosomal RNA and Tetrapod Phylogeny [PDF]
Previous phylogenetic analyses of tetrapod 18S ribosomal RNA (rRNA) sequences support the grouping of birds with mammals, whereas other molecular data, and morphological and paleontological data favor the grouping of birds with crocodiles. The 18S rRNA gene has consequently been considered odd, serving as "definitive evidence of different genes ...
Xuhua, Xia, Zheng, Xie, Karl M, Kjer
openaire +2 more sources
Abstrak Gen penyandi 18S ribosomal RNA (18S rRNA) dan ubiquitin merupakan anggota dari housekeeping genes yang sering digunakan sebagai kontrol internal pada analisis ekspresi. Penelitian bertujuan mengisolasi kedua gen tersebut pada tanaman Pandanus sp asal Riau.
Dewi Indriyani Roslim +2 more
openalex +4 more sources

