Recent Advances on the Structure and Function of RNA Acetyltransferase Kre33/NAT10
Cells, 2019 Ribosome biogenesis is one of the most energy demanding processes in the cell. In eukaryotes, the main steps of this process occur in the nucleolus and include pre-ribosomal RNA (pre-rRNA) processing, post-transcriptional modifications, and assembly of ...
Sophie Sleiman, Francois Dragon
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Frontiers in Veterinary Science, 2023 Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui +13 more
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Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]
, 2016 This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John +5 more
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A parsimonay analysis of eukaryotic small subunit ribosomal RNA (″18 S″) sequences [PDF]
Archives Internationales de Physiologie, de Biochimie et de Biophysique, 1993 Using the principles of Felsenstein's DNA parsimony analysis program (package PHYLIP 3.2), a program with the capacity to handle data sets of the magnitude of ss rRNA has been developed. With this program we applied an heuristic approach to 83 s rRNA sequences from the Antwerp data bank, compared along 4230 positions of their alignment.
Arrotin, P., Demoulin, Vincent
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Use of Internal Controls to Increase Quantitative Capabilities of the Ribonuclease Protection Assay
BioTechniques, 1997 Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR ...
Mary Jo J. Davis +2 more
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Karyotypic analysis and isolation of four DNA markers of the scleractinian coral Favites pentagona (Esper, 1795) (Scleractinia, Anthozoa, Cnidaria) [PDF]
Comparative Cytogenetics, 2022 We performed conventional and molecular cytogenetic studies on the Favites pentagona Esper, 1795, a scleractinian coral mostly found along the west coast of Japan. Karyotype analysis of F.
Rei Kawakami +6 more
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The terminal sequences of Bombyx mori 18S ribosomal RNA
Nucleic Acids Research, 1975 The 5' and 3' terminal T1 oligonucleotides of 32p-labelled B. mori 18S ribosomal RNA were isolated by a two dimensional electrophoretic (diagonal) technique. Nucleotide sequence analysis showed that the 3' terminal fragment, (G)AUCAUUAOH, is identical to that previously obtained from the 18S rRNA of several other eukaryotic species. The sequence of the
K U, Sprague, R A, Kramer, M B, Jackson
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Validation of suitable internal control genes for expression studies in aging. [PDF]
, 2009 Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
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Genomic organization of nucleolin gene in carp fish: Evidence for several genes [PDF]
, 2006 http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017&lng=es&nrm=isoThe protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals.
Alvarez, Marco +5 more
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Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress
Scientific Reports, 2022 As a powerful and attractive method for detecting gene expression, qRT-PCR has been broadly used in aquaculture research. Understanding the biology of taimen (Hucho taimen) has drawn increasing interest because of its ecological and economic value ...
Xiaoxing Yang +9 more
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