Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal [PDF]
A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central ...
Castillo, P +4 more
core +1 more source
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems.
Yong Wang +4 more
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Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere
Stephen J. Taerum +3 more
doaj +1 more source
The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]
The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert +3 more
core +1 more source
18S rRNA: A Tale of the Tail [PDF]
More than 40 years ago, the Nomura group seduced the ribosome community with the breath-takingly simple reconstitution of the 30S ribosomal subunit from its component RNA and proteins (1). However, as with many seductions, the morning-after reality is sobering.
openaire +2 more sources
Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction [PDF]
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania ...
Barroso, Paola Andrea +11 more
core +1 more source
Novel Cryptosporidium genotype in wild Australian mice (Mus domesticus) [PDF]
A total of 250 mouse fecal specimens collected from crop farms in Queensland, Australia, were screened for the presence of Cryptosporidium spp. using PCR.
Foo, C. +4 more
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Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest
Background Plasmodium species of non-human primates (NHP) are of great interest because they can naturally infect humans. Plasmodium simium, a parasite restricted to the Brazilian Atlantic Forest, was recently shown to cause a zoonotic outbreak in the ...
Lara Cotta Amaral +12 more
doaj +1 more source
Validation of suitable internal control genes for expression studies in aging. [PDF]
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA +9 more
core +1 more source
An RNA Conformational Switch Regulates Pre-18S rRNA Cleavage [PDF]
To produce mature ribosomal RNAs (rRNAs), polycistronic rRNA transcripts are cleaved in an ordered series of events. We have uncovered the molecular basis for the ordering of two essential cleavage steps at the 3'-end of 18S rRNA. Using in vitro and in vivo structure probing, RNA binding and cleavage experiments, and yeast genetics, we demonstrate that
Allison C, Lamanna, Katrin, Karbstein
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