Results 31 to 40 of about 608,629 (167)

Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies

Malaria Journal, 2021
Background Plasmodium 18S rRNA is a sensitive biomarker for detecting Plasmodium infection in human blood. Dried blood spots (DBS) are a practical sample type for malaria field studies to collect, store, and transport large quantities of blood samples ...
Ming Chang, Selena Johnston, Annette M. Seilie, Dianna Hergott, Sean C. Murphy   +4 more
doaj   +1 more source

Mass Fractal Dimension of the Ribosome and Implication of its Dynamic Characteristics [PDF]

Physical Review E 73, 042901 (2006), 2006
Self-similar properties of the ribosome in terms of the mass fractal dimension are investigated. We find that both the 30S subunit and the 16S rRNA have fractal dimensions of 2.58 and 2.82, respectively; while the 50S subunit as well as the 23S rRNA has the mass fractal dimension close to 3, implying a compact three dimensional macromolecule.
arxiv   +1 more source

Characterization of the two intra-individual sequence variants in the 18S rRNA gene in the plant parasitic nematode, Rotylenchulus reniformis. [PDF]

PLoS ONE, 2013
The 18S rRNA gene is fundamental to cellular and organismal protein synthesis and because of its stable persistence through generations it is also used in phylogenetic analysis among taxa.
Seloame T Nyaku, Venkateswara R Sripathi, Ramesh V Kantety, Yong Q Gu, Kathy Lawrence, Govind C Sharma   +5 more
doaj   +1 more source

Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination [PDF]

Proc. Natl. Acad. Sci. USA 105, 18159-18164 (2008), 2008
In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E.
arxiv   +1 more source

Synergistic interaction network between the snR30 RNP, Utp23, and ribosomal RNA during ribosome synthesis

RNA Biology, 2022
snR30/U17 is a highly conserved H/ACA RNA that is required for maturation of the small ribosomal subunit in eukaryotes. By base-pairing to the expansion segment 6 (ES6) of 18S ribosomal RNA (rRNA), the snR30 H/ACA Ribonucleoprotein (RNP) indirectly ...
Timothy J. Vos, Ute Kothe
doaj   +1 more source

Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Microbiome, 2018
Background Targeted amplicon deep sequencing (TADS) of the 16S rRNA gene is commonly used to explore and characterize bacterial microbiomes. Meanwhile, attempts to apply TADS to the detection and characterization of entire parasitic communities have been
Briana R. Flaherty, Eldin Talundzic, Joel Barratt, Kristine J. Kines, Christian Olsen, Meredith Lane, Mili Sheth, Richard S. Bradbury   +7 more
doaj   +1 more source

Parasites and blood-meal hosts of the tsetse fly in Tanzania: a metagenomics study

Parasites & Vectors, 2022
Background Tsetse flies can transmit various Trypanosoma spp. that cause trypanosomiasis in humans, wild animals, and domestic animals. Amplicon deep sequencing of the 12S ribosomal RNA (rRNA) gene can be used to detect mammalian tsetse hosts, and the ...
Ju Yeong Kim, Jun Ho Choi, Sung-Hyun Nam, Robert Fyumagwa, Tai-Soon Yong   +4 more
doaj   +1 more source

Burkholderia genome mining for nonribosomal peptide synthetases reveals a great potential for novel siderophores and lipopeptides synthesis [PDF]

MicrobiologyOpen, 2016, 5 (3), pp.512 - 526, 2016
Burkholderia is an important genus encompassing a variety of species, including pathogenic strains as well as strains that promote plant growth. We have carried out a global strategy, which combined two complementary approaches. The first one is genome guided with deep analysis of genome sequences and the second one is assay guided with experiments to ...
arxiv   +1 more source

Partial methylation at Am100 in 18S rRNA of baker's yeast reveals ribosome heterogeneity on the level of eukaryotic rRNA modification.

PLoS ONE, 2014
Ribosome heterogeneity is of increasing biological significance and several examples have been described for multicellular and single cells organisms.
Markus Buchhaupt, Sunny Sharma, Stefanie Kellner, Stefanie Oswald, Melanie Paetzold, Christian Peifer, Peter Watzinger, Jens Schrader, Mark Helm, Karl-Dieter Entian   +9 more
doaj   +1 more source

Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences [PDF]

, 2012
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing protocols are, however, limited by the fraction of the 16S rRNA gene that is interrogated and ...
arxiv   +1 more source