Results 31 to 40 of about 120,971 (346)

Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal [PDF]

open access: yes, 2018
A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central ...
Castillo, P   +4 more
core   +1 more source

Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis.

open access: yesPLoS ONE, 2014
Eukaryotic 18S ribosomal RNA (rRNA) gene primers that feature a wide coverage are critical in detecting the composition of eukaryotic microscopic organisms in ecosystems.
Yong Wang   +4 more
doaj   +1 more source

Validation of a PNA Clamping Method for Reducing Host DNA Amplification and Increasing Eukaryotic Diversity in Rhizosphere Microbiome Studies

open access: yesPhytobiomes Journal, 2020
Protists and microscopic animals are important but poorly understood determinants of plant health. Plant-associated eukaryotes could be surveyed by high-throughput sequencing of 18S ribosomal RNA (rRNA) genes but the abundance of plant DNA in rhizosphere
Stephen J. Taerum   +3 more
doaj   +1 more source

The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron [PDF]

open access: yes, 1992
The nucleotide sequence of the gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis was determined. It revealed the presence of a group I intron with a length of 411 nucleotides.
De Wachter, Rupert   +3 more
core   +1 more source

18S rRNA: A Tale of the Tail [PDF]

open access: yesJournal of Molecular Biology, 2011
More than 40 years ago, the Nomura group seduced the ribosome community with the breath-takingly simple reconstitution of the 30S ribosomal subunit from its component RNA and proteins (1). However, as with many seductions, the morning-after reality is sobering.
openaire   +2 more sources

Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction [PDF]

open access: yes, 2005
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania ...
Barroso, Paola Andrea   +11 more
core   +1 more source

Novel Cryptosporidium genotype in wild Australian mice (Mus domesticus) [PDF]

open access: yes, 2007
A total of 250 mouse fecal specimens collected from crop farms in Queensland, Australia, were screened for the presence of Cryptosporidium spp. using PCR.
Foo, C.   +4 more
core   +2 more sources

Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest

open access: yesMalaria Journal, 2023
Background Plasmodium species of non-human primates (NHP) are of great interest because they can naturally infect humans. Plasmodium simium, a parasite restricted to the Brazilian Atlantic Forest, was recently shown to cause a zoonotic outbreak in the ...
Lara Cotta Amaral   +12 more
doaj   +1 more source

Validation of suitable internal control genes for expression studies in aging. [PDF]

open access: yes, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA   +9 more
core   +1 more source

An RNA Conformational Switch Regulates Pre-18S rRNA Cleavage [PDF]

open access: yesJournal of Molecular Biology, 2011
To produce mature ribosomal RNAs (rRNAs), polycistronic rRNA transcripts are cleaved in an ordered series of events. We have uncovered the molecular basis for the ordering of two essential cleavage steps at the 3'-end of 18S rRNA. Using in vitro and in vivo structure probing, RNA binding and cleavage experiments, and yeast genetics, we demonstrate that
Allison C, Lamanna, Katrin, Karbstein
openaire   +2 more sources

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