Renal pelvis urobiome dysbiosis is associated with postoperative systemic inflammatory response syndrome after percutaneous nephrolithotomy [PDF]
Early prediction and diagnosis of systemic inflammatory response syndrome (SIRS) following percutaneous nephrolithotomy (PCNL) are critical. This study aimed to investigate differences in clinical characteristics and the renal pelvis urobiome between ...
Qing Wang +5 more
doaj +2 more sources
Incipient Range Expansion of Green Turtles in the Mediterranean. [PDF]
ABSTRACT In response to global climate change, numerous taxa are expanding their living ranges. In highly migratory species such as sea turtles, this expansion may be driven by individuals from nearby or distant areas. Recent nests outside the species' typical nesting range and reports of adult‐sized individuals in the western Mediterranean suggest a ...
Marín-Capuz G +12 more
europepmc +2 more sources
Association between ocular surface microbiota species and type 2 diabetes mellitus with or without retinopathy and related clinical parameters [PDF]
Diabetic retinopathy (DR) is the most prevalent blinding microvascular complication of diabetes mellitus. Although altered ocular surface microbiota (OSM) has been recently linked to type 2 diabetes mellitus (DM) and diabetic retinopathy (DR), its ...
Jihong Wang +5 more
doaj +2 more sources
Integrated analysis of the aqueous humor microbiome and lens capsule transcriptome in high myopia cataract: a pilot study [PDF]
IntroductionHigh myopia is a critical risk factor that accelerates cataract onset and increases surgical complexity, yet the underlying mechanisms remain incompletely understood.MethodsIn this study, we collected aqueous humor samples (6 from high myopia
Yaoyan Qiu +24 more
doaj +2 more sources
Escherichia coli promotes colorectal cancer metastasis by maintaining enhancer-promoter loops through releasing neutrophil extracellular traps [PDF]
The involvement of intestinal microbiota in the process of neutrophil-mediated colorectal cancer liver metastasis (CRCLM) is not yet fully understood. Here, we show that Escherichia coli (E. coli) is prevalent in CRC tissues with LM using 2bRAD-M-Seq and
Banglun Pan +7 more
doaj +2 more sources
Critically ill patients will benefit from single isolated laminar-air-flow wards by improving the environmental microbial composition [PDF]
Background Hospital-associated infection (HAI) is an important issue in intensive care units (ICUs). We still lack direct evidence on whether the ICU patients and/or the medical system can benefit from single isolated laminar-air-flow (LAF) wards ...
Tianji Zhang +6 more
doaj +2 more sources
A lung microbial signature for postoperative recurrence in stage I–II non-small cell lung cancer [PDF]
Background Postoperative recurrence remains a prevalent and lethal threat to survival in early-stage non-small cell lung cancer (NSCLC), affecting approximately 20% of patients within five years. While emerging evidence implicates the lung microbiota and
Chunyan Gu +12 more
doaj +2 more sources
Species-resolved sequencing of low-biomass microbiomes by 2bRAD-M [PDF]
Abstract Microbiome samples with low microbial biomass or severe DNA degradation remain challenging for amplicon-based (e.g., 16S/18S-rRNA) or whole-metagenome sequencing (WMS) approaches. Here, we introduce 2bRAD-M, a highly reduced and cost-effective metagenome-sequencing strategy which only sequences ~1% of ...
Zheng Sun +15 more
openaire +2 more sources
Microbial signatures of neonatal bacterial meningitis from multiple body sites
As a common central nervous system infection in newborns, neonatal bacterial meningitis (NBM) can seriously affect their health and growth. However, although metagenomic approaches are being applied in clinical diagnostic practice, there are some ...
Yuyang Hou +9 more
doaj +1 more source
High-Resolution Microbial Fingerprinting for Forensic Individual Identification: A Proof-of-Concept Study Integrating 2bRAD-M and Hierarchical Attention Network. [PDF]
Background: Human skin and saliva microbial communities have emerged as promising forensic biomarkers due to their individual specificity. However, existing studies are limited by small sample sizes and methodological inconsistencies. This proof-of-concept study aims to develop a novel framework integrating 2bRAD-M sequencing with a hierarchical ...
Li H +10 more
europepmc +2 more sources

