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Circadian Profiling of the Arabidopsis Proteome Using 2D-DIGE [PDF]

open access: yesFrontiers in Plant Science, 2016
Clock-generated biological rhythms provide an adaptive advantage to an organism, result in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under ...
David E Somers   +2 more
exaly   +4 more sources

2D-DIGE based proteome analysis of wheat-Thinopyrum intermedium 7XL/7DS translocation line under drought stress [PDF]

open access: yesBMC Genomics, 2022
Background Drought stress is the most limiting factor for plant growth and crop production worldwide. As a major cereal crop, wheat is susceptible to drought. Thus, discovering and utilizing drought-tolerant gene resources from related species are highly
Fengkun Lu   +6 more
doaj   +2 more sources

Proteomic changes during B cell maturation: 2D-DIGE approach. [PDF]

open access: yesPLoS ONE, 2013
B cells play a pivotal role in adaptive immune system, since they maintain a delicate balance between recognition and clearance of foreign pathogens and tolerance to self.
Johanna Salonen   +3 more
doaj   +2 more sources

2D DIGE does not reveal all: A scotopic report suggests differential expression of a single Calponin family member protein for tetany of sphincters! [PDF]

open access: yesFrontiers in Medicine, 2015
Using 2D differential gel electrophoresis (DIGE) and MS (mass spectrometry), a recent report by Rattan and Ali (2015) compared proteome expression between tonically contracted sphincteric smooth muscles of the internal anal sphincter (IAS), in comparison
Arun eChaudhury
doaj   +2 more sources

A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection

open access: yesCells, 2023
Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-up shotgun has become more popular than gel-based top-down proteomics.
Huriye Ercan   +9 more
doaj   +1 more source

Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2015
Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The purpose of this
Jae Eun Lee   +5 more
doaj   +1 more source

Serum protein signatures differentiating autoimmune pancreatitis versus pancreatic cancer. [PDF]

open access: yesPLoS ONE, 2013
Autoimmune pancreatitis (AIP) is defined by characteristic lymphoplasmacytic infiltrate, ductal strictures and a pancreatic enlargement or mass that can mimic pancreatic cancer (PaCa). The distinction between this benign disease and pancreatic cancer can
Klaus Felix   +9 more
doaj   +1 more source

Identification of cellular calcium binding protein calmodulin as a regulator of rotavirus A infection during comparative proteomic study. [PDF]

open access: yesPLoS ONE, 2013
Rotavirus (RV) being the major diarrhoegenic virus causes around 527000 children death (
Shiladitya Chattopadhyay   +9 more
doaj   +1 more source

An Optimized Fluorescence-Based Bidimensional Immunoproteomic Approach for Accurate Screening of Autoantibodies. [PDF]

open access: yesPLoS ONE, 2015
Serological proteome analysis (SERPA) combines classical proteomic technology with effective separation of cellular protein extracts on two-dimensional gel electrophoresis, western blotting, and identification of the antigenic spot of interest by mass ...
Virginie Dutoit-Lefèvre   +10 more
doaj   +1 more source

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