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2D DIGE of the cerebrospinal fluid proteome in neurological diseases
Expert Review of Proteomics, 20102D DIGE is a promising approach to comparative proteome analysis known for a high sensitivity and high reproducibility compared with classical 2DE techniques. It offers new possibilities for the detection of cerebrospinal fluid (CSF) biomarkers in neurological diseases, such as dementia, amyotrophic lateral sclerosis or multiple sclerosis.
Hayrettin Tumani +2 more
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Analysis of the Saliva Proteome Using 2D-DIGE
2022Saliva is now an established biofluid with a number of important applications in use across research and clinical settings. Saliva contains an assortment of biomolecules, including proteins, metabolites, RNA, DNA, and microorganisms. Numerous biomolecules enter saliva from the blood by passing through the intercellular spaces, reflecting the ...
Paul, Dowling, Eleanor M, O'Sullivan
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2017
The two-dimensional difference gel electrophoresis method is a valuable approach for proteomics. The method, using cyanine fluorescent dyes, allows the co-migration of multiple protein samples in the same gel and their simultaneous detection, thus reducing experimental and analytical time.
Matias, Pasquali +3 more
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The two-dimensional difference gel electrophoresis method is a valuable approach for proteomics. The method, using cyanine fluorescent dyes, allows the co-migration of multiple protein samples in the same gel and their simultaneous detection, thus reducing experimental and analytical time.
Matias, Pasquali +3 more
openaire +2 more sources
Sample Preparation and Protein Determination for 2D-DIGE Proteomics
2022Fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) is a widely employed method for efficient protein separation and the determination of abundance changes in distinct proteoforms. This makes this gel-based method a key technique of comparative approaches in top-down proteomics.
Stephen Gargan, Kay Ohlendieck
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Identification of Ubiquitination-Associated Proteins Using 2D-DIGE
2022Ubiquitination is a post-translational modification, in which a small regulatory protein (~8.6 kDa) is tagged as a single moiety or as a chain to target proteins. Ubiquitination is the most versatile cellular regulatory mechanism, essential to the physiological and pathophysiological cellular events that regulate protein turnover, gene transcription ...
Paul, Dowling, Despina, Bazou
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2D-DIGE and Fluorescence Image Analysis
20172D-DIGE is still a very widespread technique in proteomics for the identification of panels of biomarkers, allowing to tackle with some important drawback of classical two-dimensional gel-electrophoresis. However, once 2D-gels are obtained, they must undergo a quite articulated multistep image analysis procedure before the final differential analysis ...
ROBOTTI, Elisa, MARENGO, Emilio
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Differential Proteome Analysis Using 2D-DIGE
2012Classical two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) allows comparison and -quantitation of proteomes by visualization of protein patterns using gel stains and comparative image analysis. The introduction of fluorescent reagents for protein labeling (difference in-gel electrophoresis or DIGE) has brought substantial improvement in ...
Caroline, May +4 more
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2D DIGE for the Analysis of RAMOS Cells Subproteomes
2012Overexpression of human polμ in a Burkitt's lymphoma-derived B cell line (RAMOS), in which somatic hypermutation (SHM) is constitutive, induced an increase in somatic mutations in the parental cell line (Nucleic Acids Res 32:5861-5873, 2004). To further study Polμ implications in SHM, a dominant-negative (DN) mutant of Polμ (Polμ-DN) was generated ...
Marisol, Fernández, Juan Pablo, Albar
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2012
The technique of two-dimensional (2D) gel electrophoresis is a powerful tool for separating complex mixtures of proteins, but since its inception in the mid 1970s, it acquired the stigma of being a very difficult application to master and was generally used to its best effect by experts.
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The technique of two-dimensional (2D) gel electrophoresis is a powerful tool for separating complex mixtures of proteins, but since its inception in the mid 1970s, it acquired the stigma of being a very difficult application to master and was generally used to its best effect by experts.
openaire +2 more sources
Gel electrophoresis is a key technique of modern biochemistry and frequently used for efficient protein separation prior to top-down proteomics. For comparative studies, differential fluorescent tagging can be employed to avoid gel-to-gel variations, which has been extensively used in two-dimensional difference gel electrophoresis (2D-DIGE).
Kay, Ohlendieck, Paul, Dowling
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Kay, Ohlendieck, Paul, Dowling
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