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2D-DIGE analysis of rice proteins from different cultivars
Regulatory Toxicology and Pharmacology, 2010The 2D-DIGE (2-Dimensional Fluorescence Difference Gel Electrophoresis) method was applied to proteomic phenotyping of natural variants in 10 varieties of rice (Nipponbare, Koshihikari, Sasanishiki, Akitakomachi, Hitomebore, Hinohikari, Kasalath, Rexark, Bleiyo, Cho-ko) from the world rice collection (WRC) in the GenBank of the National Institute of ...
Reiko, Teshima +3 more
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2D-DIGE Proteomic Analysis of Mouse Liver Within 1 Week
2022Several years have passed since LC (liquid chromatography)-MS (mass spectrometry) became the mainstream for proteomic analysis; however, conventional fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their ...
Shotaro, Kamata, Isao, Ishii
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2D DIGE Analysis of Protein Extracts from Muscle Tissue
20122D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis.
C. Gelfi, S. De Palma
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Two-Dimensional Gel Electrophoresis and 2D-DIGE
2017Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the proteome of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three ...
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An Overview of 2D DIGE Analysis of Marine (Environmental) Bacteria
2012Microbes are the "unseen majority" of living organisms on Earth and main drivers of the biogeochemical cycles in marine and most other environments. Their significance for an intact biosphere is bringing environmental bacteria increasingly into the focus of genome-based science.
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Application of 2D-DIGE and iTRAQ Workflows to Analyze CSF in Gliomas
2019Proteomics is an indispensable tool for disease biomarker discovery. It is widely used for the analysis of biological fluids such as cerebrospinal fluid (CSF), blood, and saliva, which further aids in our understanding of disease incidence and progression.
Aishwarya A, Rao +3 more
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Comparative 3-Sample 2D-DIGE Analysis of Skeletal Muscles
2022The skeletal muscle proteome consists of a large number of diverse protein species with a broad and dynamic concentration range. Since mature skeletal muscles are characterized by a distinctive combination of contractile cells with differing physiological and biochemical properties, it is essential to determine specific differences in the protein ...
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2D-DIGE A Powerful Tool for Proteome Analysis
2020In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways.
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Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE
2012Metastasis remains the primary cause of cancer patient death. Although the precise molecular mechanisms at play remain largely unknown, tumor progression is currently hypothesized to follow a series of sequential steps known as the metastatic cascade.
Rommel A, Mathias +2 more
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Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
2009Two-dimensional difference gel electrophoresis (2D-DIGE) is an advanced variation of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE); protein samples are labeled with different fluorescent dyes, mixed and separated by 2D-PAGE. 2D-DIGE solves major inherent drawbacks of 2D-PAGE, demonstrating great utility in biomarker studies.
Tadashi, Kondo, Setsuo, Hirohashi
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