Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis [PDF]
BMC Genomics, 2004 Background Two-dimensional gel electrophoresis (2D-PAGE) has proven over the years to be a reliable and efficient method for separation of hundreds of proteins based on charge and mass.
Kashani-Poor Noushin +7 more
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Unitarity and Page Curve for Evaporation of 2D AdS Black Holes [PDF]
Entropy, 2022 We explore the Hawking evaporation of two-dimensional anti-de Sitter (AdS2), dilatonic black hole coupled with conformal matter, and derive the Page curve for the entanglement entropy of radiation.
Mariano Cadoni, Andrea P. Sanna
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Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
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Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis [PDF]
Proteome Science, 2011 Background Immunoprecipitation and subsequent 2D-PAGE/mass spectrometry are powerful tools to study post-translational protein modifications. Often disregarded in this workflow is the impact of the chemical cross-linker upon antibody affinity, as well as
Slupphaug Geir +3 more
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Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE. [PDF]
Mol Cancer, 2010 Background Cancer arises from normal cells through the stepwise accumulation of genetic alterations. Cancer development can be studied by direct genetic manipulation within experimental models of tumorigenesis.
Pütz SM +3 more
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2D Diagonal Redox SDS-PAGE of Proteins [PDF]
Bio-Protocol, 2013 2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).
Christian Schwarz, Joerg Nickelsen
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Jackiw-Teitelboim gravity and the models of a Hawking-Page transition for 2D black holes [PDF]
, 2020 We propose a top down construction for Jackiw-Teitelboim (JT) gravity using compactification of $ D=5 $ gravity theories in the presence of Abelian ($ U(1) $) as well as $ SU(2) $ Yang-Mills (YM) fields.
Arindam Lala +2 more
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Hawking-Page and entanglement phase transition in 2d CFT on curved backgrounds [PDF]
Journal of High Energy PhysicsThe thermodynamics and the entanglement properties of two-dimensional conformal field theories (2d CFTs) on curved backgrounds are studied. By means of conformal mapping we study the equivalent system on flat space governed by the deformed Hamiltonian ...
Akihiro Miyata +3 more
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Advances in Experimental Medicine and Biology, 2019 Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D SDS PAGE) is a method that separates proteins according to their isoelectric points in the first dimension and molecular masses in the second dimension.
Nancy C. Kendrick +4 more
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2D PAGE: Sample Preparation and Fractionation [PDF]
Methods in Molecular Biology™, 2008 Anton Posch
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