Results 251 to 260 of about 5,167,336 (292)

2D-PAGE Analysis Using Evolutionary Computation

2009
This paper presents the preliminary studies for the creation of a new tool to assist in medical diagnostic. The tool will help in the analysis of 2D-PAGE images. In order to create a 2D-PAGE image of an ideal patient—the patient could be healthy or ill—the tool will help us in the creation of an image that facilitates and speeds up future diagnostics ...
Pablo Mesejo, Enrique Fernández-Blanco, Diego Martínez-Feijóo, Francisco J. Blanco   +3 more
openaire   +1 more source

Purification and Analysis of Variant and Modified Histones Using 2D PAGE

2008
Two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) systems employing combinations of acetic acid/urea (AU), acetic acid/urea/Triton X-100 (AUT) and sodium dodecyl sulfate (SDS) gel formulations are uniquely effective for resolution of histone variants and their modified derivatives.
George R, Green, Duc P, Do
openaire   +2 more sources

Automated PreScan Function for Scanning Fluorescently Stained 2D-PAGE Gels

Journal of Proteome Research, 2005
To automate the acquisition of images from fluorescently stained gels, the power of the excitation laser(s) must be optimized for each sample to prevent spot saturation (or to allow unimportant spots to saturate) yet still retaining sensitivity. In this work, we describe the implementation and effectiveness of a pre-scan function in a robotic solution ...
Peter, Bäck, Sofia, Bengtsson, Peter, James   +2 more
openaire   +2 more sources

Sequential Analysis of N- and O-Linked Glycosylation of 2D-PAGE Separated Glycoproteins

Journal of Proteome Research, 2002
A robust method has been developed that allows analysis of both N- and O-linked oligosaccharides released from glycoproteins separated using 2D-PAGE and then electroblotted to PVDF membrane. This analysis provides efficient oligosaccharide profiling applicable to glycoproteomic analysis.
Wilson, Nicole L., Schulz, Benjamin L., Karlsson, Niclas G., Packer, Nicolle H.   +3 more
openaire   +5 more sources

Fluoreszenzmarkierung von Proteinen für 1D- und 2D-PAGE

BIOspektrum, 2013
Fluorescent pre-labelling of proteins prior to electrophoretic separations is not only advantageous for multiplex detection strategies, like DIGE (difference gel electrophoresis), but also as a general protein visualization procedure. We introduce a fluorescent amino-reactive dye which is compatible with all standard additives used for protein sample ...
Anja Griebel, Christian Obermaier, Reiner Westermeier, Martin Moche, Knut Büttner   +4 more
openaire   +1 more source

2D PAGE Databases for Proteins in Human Body Fluids

2008
With the development of the Internet, a growing number of proteomics databases have become available. The web is a powerful tool for data integration because it links the components constituting these databases, in general gel images and protein information, while offering rapid means to navigate from one database to another.
Christine Hoogland, Khaled Mostaguir, Jean-Charles Sanchez, Denis F. Hochstrasser, Ron D. Appel   +4 more
openaire   +1 more source

The mouse SWISS-2D PAGE database: a tool for proteomics study of diabetes and obesity

PROTEOMICS, 2001
A number of two-dimensional electrophoresis (2-DE) reference maps from mouse samples have been established and could be accessed through the internet. An up-to-date list can be found in WORLD-2D PAGE (http://www.expasy.ch/ch2d/2d- index.html), an index of 2-DE databases and services.
Sanchez JC, Chiappe D, Converset V, Hoogland C, Binz PA, Paesano S, Appel RD, Wang S, Sennitt M, Nolan A, Cawthorne MA, Hochstrasser DF   +11 more
openaire   +3 more sources

Interactions of nanoparticles with body proteins — improvement of 2D-PAGE-analysis by internal standard

International Journal of Pharmaceutics, 2000
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is the method of choice to investigate protein adsorption of blood proteins (opsonization) onto nanoparticular drug carriers. In general the reproducibility of the obtained adsorption patterns is satisfying.
K, Seehof, M, Kresse, K, Mäder, R H, Müller   +3 more
openaire   +2 more sources

Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE

2018
Absolute protein quantification for the analysis of proteome dynamics is more and more required by the scientific community. Therefore a number of methods have recently been reported that aim at determining concentrations of single proteins in complex samples, all of them having their advantages and limitations.
openaire   +2 more sources

2D-Page Analysis of Vietnamese Colorectal Cancer Tissue Samples

2017
Colorectal cancer is one of the five most common cancers in both men and women worldwide. This study aimed to find out the differences in the protein profile of the normal and CRC tissue samples which could aid the finding of potential biomarkers for diagnostics.
Kim Phuong Uyen Le, Phuong Uyen Vo, Kieu Minh Le, Thi Thu Hien Pham, Huu Hung Nguyen, Dang Giap Do, Minh Thong Tran, Ngoc Phuc Chau Do, Thi Thu Hoai Nguyen   +8 more
openaire   +1 more source