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Modified Metal-Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
2011Protein phosphorylation is a dynamic process of widespread regulatory significance. Phosphoproteomics attempts to provide a global view of this process during biological processes, but the approach is generally limited by the low relative amounts of phosphoproteins in biological samples.
Thomas, Colby +3 more
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Optimisation of 2D-PAGE protocols for proteomic analysis of recalcitrant plant tissues
2010Plant tissues contain relatively low amounts of proteins whose extraction is often difficult due to presence of other compounds such as rigid cellulosic cell wall, storage polysaccharides, lipids and contaminating compounds that can result in protein degradation or modification.
Križnik, Bojana +2 more
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2D-page analysis: a practical guide to principle critical parameters.
Cellular and molecular biology (Noisy-le-Grand, France), 1994Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a technique that is becoming more and more popular as the secrets of the critical parameters involved in its execution are being continually unravelled. The majority of these parameters are linked to the successful realization of the first dimension.
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Casting and Running Vertical Slab-Gel Electrophoresis for 2D-PAGE
2003B J, Walsh, B R, Herbert
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Recognition of Pornographic Web Pages by Classifying Texts and Images
IEEE Transactions on Pattern Analysis and Machine Intelligence, 2007Weiming Hu, Ou Wu, Stephen J. Maybank
exaly