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Comparison of 2n and Non-2n Pollen-Producing Haploid × Wild Species Hybrids in Potato

Journal of Heredity, 1989
Haploid × wild species hybrids (2n=2x=24) provide a means of capturing the germ plasm of the 2x, 2EBN wild Solanum spp. in a form in which it can be maintained and evaluated. 2n pollen-producing and non-2n pollen-producing hybrids were compared from the same population in regard to seven agronomic and breeding characteristics to determine appropriate ...
G. L. Yerk, S. J. Peloquin
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Discrepancy between spindle anomalies and 2N pollen production insolanum interspecific hybrids

American Potato Journal, 1997
The cytological basis of 2n pollen production was studied in a diploidSolanum progeny coming from a cross between aS. tuberosum S. chacoense hybrid which does not produce2n pollen (T710) and a S.phurejar S. tuberosum hybrid (W5295.7) which does. Both parental clones showed anomalous orientation of spindles at Metaphase II of microsporogenesis, but only
BARONE, AMALIA   +3 more
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Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus

Euphytica, 2016
Artificially inducing 2n gametes through chromosome doubling is an effective way to obtain polyploids. In this study, Eucalyptus urophylla microsporogenesis and flower development were investigated to guide 2n pollen induction. We also investigated suitable conditions for colchicine treatment. Our results showed that E.
Jun Yang   +5 more
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Production of 2n Pollen in Red Clover1

Crop Science, 1984
The success of tetraploid red clover (Trifolium pratense L.) in Europe has stimulated interest in developing tetraploid red clover adapted to the United States. The use of 2n gametes to produce tetraploid alfalfa (Medicago sativa L.) and potato (Solanum tuberosum L.) from diploid germplasm suggests that 2n gametes may be an alternative to chemical ...
W. A. Parrott, R. R. Smith
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Pollen transcriptome analysis of Solanum tuberosum (2n = 4x = 48), S. demissum (2n = 6x = 72), and their reciprocal F1 hybrids

Plant Cell Reports, 2013
Pollen mRNAs were different in reciprocal F 1 hybrids, which were probably caused by a cytoplasm-nuclear chromosomal genes interaction. We have found reciprocal differences in crossability between F1 hybrids of Solanum tuberosum (T) and a Mexican wild potato species S. demissum (D).
Rena, Sanetomo, Kazuyoshi, Hosaka
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Male fertility and 2n pollen production in haploid-wild species hybrids

American Potato Journal, 1985
Hybrids (2n = 2x = 24) from crosses between Group Tuberosum haploids (2n = 2x = 24) and 24-chromosome, wild species must be fertile and produce 2n gametes if they are to be used in breeding schemes involving sexual polyploidization. Male fertility of the 2x hybrids depends primarily on the species parent. Hybrids involvingS. berthaultii, S.
Shelley A. Hermundstad, S. J. Peloquin
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Manipulation of the 2n-gametes frequencies in Solanum pollen

Euphytica, 1988
Besides the normal gametes, some 2x potato genotypes produce 2n-gametes as well. In interploidy crosses (2x×4x, 4x×2x) 2n-gametes are necessary, whilst in intercrossing at the diploid level they are undesirable.
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Incidence and molecular markers of 2n pollen in Populus tomentosa Carr.

Euphytica, 2006
Microscopic examination, amplified fragment length polymorphism (AFLP) and SCAR (sequence-characterized amplified region) molecular markers were employed to determine the incidence of 2n pollen (unreduced pollen) in Chinese white poplar (Populus tomentosa Carr.) and to identify related molecular markers.
Zhenghai Zhang   +4 more
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An efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in Brassica napus pollen

Plant Cell Reports, 2004
A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable for flow cytometric analysis as well ...
Gang, Pan   +3 more
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2n pollen formation via parallel spindles in the potato cultivar sebago

American Potato Journal, 1980
Parallel spindles occur during microsporogenesis in the cultivar Sebago (2n=4x =48) and are the basis of 2n pollen production in this clone. The relations between the frequencies of parallel spindles, 2n microspores, and 2n pollen are striking. The predicted frequency of 2n pollen based on the percentage of microsporocytes with parallel spindles is ...
E. W. Souter, J. C. Dawe, S. J. Peloquin
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