Results 121 to 130 of about 10,459,359 (387)
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Africa Spectrum, 2010 In this farewell lecture on the occasion of his departure as Professor of Development in sub-Saharan Africa at Leiden University and Director of the African Studies Centre (ASC), Leiden, the author starts with the vuvuzela issue as an illustration of the
Leo J. de Haan
doaj
Inverse Drop Calorimeter for Enthalpy Determinations in the Range 300 to 1500 K. [PDF]
, 1972 Fredrik Grønvold +4 more
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Quality of Different Scales in an Online Survey in Mexico and Colombia
Journal of Politics in Latin America, 2015 The formulation of theories and hypotheses is done at the level of concepts. These concepts are often tested by operationalizing them using survey questions.
Melanie Revilla, Carlos Ochoa
doaj
FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
Myanmar: Political Reforms and the Recalibration of External Relations
Journal of Current Southeast Asian Affairs, 2015 This opening chapter provides some background to the domestic reform agenda, along with its drivers and motivations. From 1988 to 2011, the military built up institutions that guaranteed the military’s dominant position in the political arena.
Marco Bünte, Jörn Dosch
doaj
Surface and Bulk Structural Properties of Single Crystalline Sr3Ru2O7
, 2010 We report temperature and thermal-cycling dependence of surface and bulk structures of double-layered perovskite Sr3Ru2O7 single crystals. The surface and bulk structures were investigated using low-energy electron diffraction (LEED) and single-crystal X-
Biao Hu +8 more
core +1 more source
FEBS Open Bio, EarlyView.The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source