Effect of High‐Intensity Interval Training With Varying Work‐to‐Rest Ratios on Specific Physical Fitness in Adolescent Taekwondo Athletes [PDF]
European Journal of Sport ScienceThis study investigated the effects of high‐intensity interval training (HIIT) with different work‐to‐rest ratios during the same training duration on the specific physical fitness of Chinese adolescent taekwondo athletes (N = 30, 16–20 years, 18 males ...
Ning Xu, Yadong Xue, Meng Zhang
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Staphylococcus aureus 30S Ribosomal Subunit Purification and Its Biochemical and Cryo-EM Analysis
Bio-Protocol, 2022 The ribosome is a complex cellular machinery whose solved structure allowed for an incredible leap in structural biology research. Different ions bind to the ribosome, stabilizing inter-subunit interfaces and structurally linking rRNAs, proteins, and ...
Margarita Belinite +2 more
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Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation [PDF]
Nature CommunicationsIn many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that
Johann J. Roske +6 more
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An Additional 30-s Observation of the Right-Sided Colon Using a Novel Endoscopic System with Texture and Color Enhancement Imaging Decreases Polyp Miss Rates: A Multicenter Study [PDF]
DiagnosticsBackground/Objectives: White light imaging (WLI) of colonoscopy has a 26% adenoma miss rate. We aimed to evaluate the effectiveness of an additional 30 s (Add-30s) observation of the right-sided colon using a novel system (EVIS X1; Olympus Co.) with ...
Yoshikazu Inagaki +12 more
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Abstract P-31: Assembly of the Complex of the 30S Ribosomal Subunit and the Ribosome Maturation Factor P from Staphylococcus aureus for Structural Studies by Cryo-Electron Microscopy [PDF]
International Journal of Biomedicine, 2021 Background: Staphylococcus aureus (S. aureus) is one of the main human pathogens causing numerous nosocomial soft tissue infections and is among the best-known causes of bacterial infections.
Natalia Garaeva +5 more
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Abstract P-21: The Investigation of S.aureus Ribosome-Binding Factor A Localization on the 30S Ribosomal Subunit by Cryo-Electron Microscopy [PDF]
International Journal of Biomedicine, 2021 Background: Ribosome biogenesis is a complex process of ribosomal RNA and protein binding. Bacterial ribosome maturation and components involved in it are especially interesting, because they are widespread targets for antibiotics.
Aydar G. Bikmullin +7 more
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Abstract P-27: The 30S Ribosomal Subunit Assembly Factor Rbfa Plays a Key Role in the Formation of the Central Pseudoknot and in the Correct Docking of Helix 44 of the Decoding Center [PDF]
International Journal of Biomedicine, 2021 Background: Ribosome biogenesis is a complicated multi-stage process. In the cell, 30S ribosomal subunit assembly is fast and efficient, proceeding with the help of numerous assembly protein factors.
Elena M. Maksimova +7 more
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Abstract P-26: Staphylococcus Aureus 30S Ribosomal Subunit in a Complex with the Era GTPase: Sample Preparation for Cryo-EM [PDF]
International Journal of Biomedicine, 2021 Background: An essential in bacteria GTPase Era is a multifunctional protein that is involved in cell cycle regulation and appears to play a significant role in ribosome biogenesis. It is required for the maturation of the 30S ribosomal subunit.
Evelina Klochkova +5 more
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A Complementary Mechanism of Bacterial mRNA Translation Inhibition by Tetracyclines
Frontiers in Microbiology, 2021 Tetracycline has positively impacted human health as well as the farming and animal industries. Its extensive usage and versatility led to the spread of resistance mechanisms followed by the development of new variants of the antibiotic.
Victor Barrenechea +4 more
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The Cryo-EM structure of a complete 30S translation initiation complex from Escherichia coli. [PDF]
PLoS Biology, 2011 Formation of the 30S initiation complex (30S IC) is an important checkpoint in regulation of gene expression. The selection of mRNA, correct start codon, and the initiator fMet-tRNA(fMet) requires the presence of three initiation factors (IF1, IF2, IF3 ...
Patricia Julián +6 more
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