Results 91 to 100 of about 444 (134)

A procedure for the preparation of [32P]phosphatidic acid

Analytical Biochemistry, 1986
The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in
R K, Keller, W L, Adair, N, Cafmeyer
openaire   +2 more sources

A route for the preparation of [32P]‐sodium monofluorophosphate

Journal of Labelled Compounds and Radiopharmaceuticals, 1999
[32P]-labelled sodium monofluorophosphate (Na2FPO3) was prepared according to a procedure, which is based on melting of a mixture of carrier-added [32P]-phosphoric acid and ammonium bifluoride, both solid. Monofluorophosphate was precipitated as the silver salt from the reaction mixture, then it was converted to the sodium salt by using a cation ...
B. Wierczinski, Z. I. Kolar, E. P. Sedlick   +2 more
openaire   +1 more source

False-Positive 32P Uptake Tests

Archives of Ophthalmology, 1978
Three patients had suspected choroidal melanoma and positive results for radioactive phosphorus (32P) uptake tests, leading to enuclearion in two of the patients. No malignancy was present on histopathologic examination. This experience reemphasizes the need for the ophthalmologist to evaluate the total clinical picture and not to give inappropriate ...
Z N, Zakov, T R, Smith, D M, Albert
openaire   +2 more sources

Absorption and Translocation of Phosphate-32P

1974
Terrestrial seed plants require approximately 12 mineral elements, in addition to nitrogen, which they absorb as inorganic ions through their roots from the soil. Ion absorption occurs by two basic processes commonly known as passive and active ion uptake.
openaire   +1 more source

32P-Postlabeling Analysis of DNA Adducts

2014
32P-Postlabeling analysis is an ultra-sensitive method for the detection of DNA adducts, such as those formed directly by the covalent binding of carcinogens and mutagens to bases in DNA, and other DNA lesions resulting from modification of bases by endogenous or exogenous agents (e.g., oxidative damage).
Phillips, David H, Arlt, Volker M
openaire   +4 more sources

Effects of 32P decay on enzyme synthesis

Journal of Molecular Biology, 1961
Cultures of Escherichia coli 15T − inactivated by 32 P decay to a survival of 0·1% are capable of synthesizing phosphatase at about 25% of the rate found in completely viable cells. These cultures also retain a similar fraction of their ability to form β -galactosidase, if no energy source is present during the induction.
openaire   +2 more sources

Application of HPLC in the 32P-postlabeling assay

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993
The postlabeling procedure for the detection of DNA modifications entails enzyme-catalyzed incorporation of 32P into nucleotides and chromatographic separation of radiolabeled products for quantification. Alternate versions of this procedure have been developed which vary in sensitivity and in applicability for the detection of different DNA adducts ...
openaire   +2 more sources

Preparation of [β-32P] ATP and [γ-32P] ATP

Biochimica et Biophysica Acta, 1958
Ryō Tanaka, Yoshitake Mano, Norio Shimazono   +2 more
openaire   +1 more source

Abstract 32P

Plastic and Reconstructive Surgery, 2012
S Shakir, JJ Cray, JE Losee, GM Cooper
openaire   +1 more source

Results of a single-arm pilot study of 32P microparticles in unresectable locally advanced pancreatic adenocarcinoma with gemcitabine/nab-paclitaxel or FOLFIRINOX chemotherapy

ESMO Open, 2022
Elisa Young, Nam Nguyen, Paul Ross
exaly