Results 171 to 180 of about 149,531 (197)
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A procedure for the preparation of [32P]phosphatidic acid

Analytical Biochemistry, 1986
The phosphorylation procedure of F. Cramer, W. Rittersdorf, and W. Bohm [(1961) Chem. Ber. 654, 180] using bis(triethylammonium) phosphate and trichloroacetonitrile was shown to be effective in the synthesis of [32P]phosphatidic acid. From diacylglyceride and 0.5 mCi H(3)32PO4, 25-50 microCi of labeled material (sp act = 1 mCi/mumol) can be prepared in
R K Keller, Nancy Cafmeyer, W L Adair
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Metabolic studies of 32P-labelled triethylenethiophosphoramide

Biochemical Pharmacology, 1959
Abstract Triethylenethiophosphoramide (ThioTEPA) labelled with radioactive phosphorus has been prepared and its metabolism examined in the rat, mouse, dog and rabbit. The drug was metabolized rapidly in vivo . The primary stage was the rapid replacement of S by O with the formation of TEPA. The mouse was exceptional in that the compound was degraded
B. W. Fox, A. W. Craig, Harold Jackson
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[7] Autoradiograms: 35S and 32P

1987
Autoradiography in the strict sense refers to the exposure of film by radioactive particles, whereas fluorography refers to the exposure of film by light generated from the interaction of radioactive particles with added compounds called fluors. Whether fluors are mixed with or are external to the sample depends on the energy and range of the emissions.
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The 32P-postlabeling assay for DNA adducts [PDF]

open access: possibleNature Protocols, 2007
32P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen-DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3'-monophosphates; (ii) enrichment of the adduct fraction of the DNA digest; (iii) 5'-labeling of the adducts by transfer of 32P-orthophosphate from [gamma ...
Phillips, David H, Arlt, Volker M
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Synthesis of [γ-32P]thiamine triphosphate

Analytical Biochemistry, 1988
We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate.
Ernest Schoffeniels   +3 more
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The absolute standardization of 32P and 204Tl at LNMRI

Applied Radiation and Isotopes, 2004
32P and 204Tl solutions were standardized within the frame of the international key comparisons organized by bureau international des poids et mesures, in 2002. The activity concentration of 32P was measured by counting solid sources in a 4pibeta proportional gas flow counter and by liquid scintillation counting.
Carlos José da Silva   +3 more
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Bioavailability and metabolism of [32P]malathion in the hen

Environmental Research, 1976
Abstract In this study separation of urine and faeces was made possible in the hen and bioavailability and metabolism of [ 32 P] malathion were studied after a single oral dose of 262.4 mg/kg. The results suggested that the compound is absorbed fairly rapidly from the gastrointestinal tract, significant quantities being detected in plasma and blood ...
P.K. Gupta, B.S. Paul
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False-Positive 32P Uptake Tests

Archives of Ophthalmology, 1978
Three patients had suspected choroidal melanoma and positive results for radioactive phosphorus (32P) uptake tests, leading to enuclearion in two of the patients. No malignancy was present on histopathologic examination. This experience reemphasizes the need for the ophthalmologist to evaluate the total clinical picture and not to give inappropriate ...
Z. Nicholas Zakov   +2 more
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A route for the preparation of [32P]‐sodium monofluorophosphate

Journal of Labelled Compounds and Radiopharmaceuticals, 1999
[32P]-labelled sodium monofluorophosphate (Na2FPO3) was prepared according to a procedure, which is based on melting of a mixture of carrier-added [32P]-phosphoric acid and ammonium bifluoride, both solid. Monofluorophosphate was precipitated as the silver salt from the reaction mixture, then it was converted to the sodium salt by using a cation ...
B. Wierczinski   +2 more
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Absorption and Translocation of Phosphate-32P

1974
Terrestrial seed plants require approximately 12 mineral elements, in addition to nitrogen, which they absorb as inorganic ions through their roots from the soil. Ion absorption occurs by two basic processes commonly known as passive and active ion uptake.
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