Results 181 to 190 of about 149,531 (197)

Spins of levels in 32P and 34S

Nuclear Physics A, 1970
Abstract The method II angular-correlation technique of Litherland and Ferguson has been applied to the reactions 29 Si(α, pγ) 32 P and 31 P(α, pγ) 34 S. In 32 P the state at 1.755 MeV was determined to have J = 3. In 34 S the state at 4.07 MeV was determined to have J = 1, and the state at 4.89 MeV, J = 2. For other low-lying levels in 32
N. R. Roberson, D. R. Tilley, C. E. Moss, R. V. Poore   +3 more
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Effects of 32P decay on enzyme synthesis

Journal of Molecular Biology, 1961
Cultures of Escherichia coli 15T − inactivated by 32 P decay to a survival of 0·1% are capable of synthesizing phosphatase at about 25% of the rate found in completely viable cells. These cultures also retain a similar fraction of their ability to form β -galactosidase, if no energy source is present during the induction.
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An enzymatic method for [32P]phosphoenolpyruvate synthesis

Analytical Biochemistry, 1983
Abstract A convenient method for the enzymatic synthesis of [32P]phosphoenolpyruvate from [γ-32P]ATP using partially pufified phosphoenolpyruvate carboxykinase from Escherichia coli is described. The synthesis was shown to convert essentially all the [γ-32P]ATP to [32P]phosphoenolpyruvate, which was subsequently separated from residual [γ-32P]ATP and
Roshan L. Mattoo, E. Bruce Waygood
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Plasma membrane-associated phosphatase activities hydrolyzing [32P]phosphotyrosyl histones and [32P]phosphatidylinositol phosphate

Analytical Biochemistry, 1987
We describe a procedure of preparing [32P]phosphotyrosyl histones with minimal contamination by 32P-labeled lipids; the latter was usually found to be mixed with the phosphoproteins when the cell membrane-enriched fraction of A-431 cells was used as a source of tyrosine kinase.
Susan S. Imes, Aileen F. Knowles, Nathan O. Kaplan   +2 more
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Enzymatic synthesis of 32P-labeled coenzyme A

Analytical Biochemistry, 1974
Abstract A method for the enzymatic synthesis and purification of [3′- 32 P]coenzyme. A is described.
O.H. Wieland, E.A. Siess
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The incorporation of 32P-labelled orthophosphate into nucleotides

Biochimica et Biophysica Acta, 1954
Abstract 1. 2. Chemical fractionation, column and paper chromatographic methods have beee employed to study the short-term incorporation of 32P-orthophosphate into various nucleotides and other phosphorylated compounds in respiring rabbit liver homogenates. 2. 2.
Vernon H. Cheldelin, H.R. Mahler, Lester J. Teply, E. Dimant, Frank M. Huennekens, Gerson Jacobs, Jesse C. Rabinowitz   +6 more
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Application of HPLC in the 32P-postlabeling assay

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1993
The postlabeling procedure for the detection of DNA modifications entails enzyme-catalyzed incorporation of 32P into nucleotides and chromatographic separation of radiolabeled products for quantification. Alternate versions of this procedure have been developed which vary in sensitivity and in applicability for the detection of different DNA adducts ...
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Preparation of [β-32P] ATP and [γ-32P] ATP

Biochimica et Biophysica Acta, 1958
Yoshitake Mano, Ryō Tanaka, Norio Shimazono   +2 more
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[32P]Pi uptake in cultured fibroblasts and osteoblasts: most of the cellular 32P is not Pi

Biochemical Society Transactions, 1989
Graham J. Kemp, Alan Bevington, Hamed I. Khouja, R. Graham G. Russell   +3 more
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Preparation of 32P and 35S labelled coenzme A

Biochimica et Biophysica Acta, 1955
A.C. Leopold, S.P. Sen
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