Internal 32P-labeling of L-deoxyoligonucleotides [PDF]
Nucleic Acids Research, 2003 A general two step procedure for the internal labeling of L-deoxyoligonucleotides, Spiegelmers, has been developed. Through radioactive labeling oligonucleotides can easily be detected and monitored in biological samples. T4 polynucleotide kinase is shown to efficiently phosphorylate strands of L-nucleic acids which allows the labeling with phosphorous
Christian, Frauendorf +5 more
openaire +2 more sources
Origin of phospholipids in the chick embryo during development
Journal of Lipid Research, 1965 Inorganic-32P, injected into yolks of eggs incubated to produce embryos of different ages, was incorporated into all phospholipid fractions in both whole chick embryo and embryo brain. Specific activity values compared between individual phospholipids of
T.J. Siek, R.W. Newburgh
doaj +1 more source
Outcomes of Phosphorus-32 Intracavitary Irradiation for Craniopharyngiomas: A Review of the Literature [PDF]
International Clinical Neuroscience Journal, 2016 Purpose: To review on outcomes of Phosphorus-32 (32P) intracavitary irradiation for Craniopharyngiomas. Methods: A literature review of all full publications in English biomedical journals (1981-2014) was performed.
Parisa Azimi +2 more
doaj +1 more source
Insulin Action on Polyunsaturated Phosphatidic Acid Formation in Rat Brain: An “In Vitro” Model with Synaptic Endings from Cerebral Cortex and Hippocampus [PDF]
, 2009 The highly efficient formation of phosphatidic acid from exogenous 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG) in rat brain synaptic nerve endings (synaptosomes) from cerebral cortex and hippocampus is reported.
Giusto, Norma Maria +2 more
core +1 more source
Desenvolvimento de técnicas de bioanálise in vitro para monitoração individual de 32P
Brazilian Journal of Radiation Sciences, 2017 The 32P is used in the form of liquid unsealed sources in medical facilities, research and teaching, representing a risk of internal exposure in routine activities and in case of accidental incorporation.
Ana Letícia Almeida Dantas +3 more
doaj +1 more source
APE1 Activity is Controlled by Non-G-Quadruplex Conformations in Single- and Double-Stranded G-Quadruplex Constructs. [PDF]
ChemistryG‐quadruplex (G4) folding in DNA is inhibitory to AP endonuclease, although the protein still binds well to G4s. Curiously, partially folded G‐rich sequences, possibly transient hairpins, containing AP are very good substrates for cleavage by APE1. Herein, the enzyme activity and G4 folding are studied in single‐stranded vs. duplex contexts.
Trabucco BL, Fleming AM, Burrows CJ.
europepmc +2 more sources
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Advanced Science, EarlyView.CircMT‐RNR2 promotes wound healing by enhancing fibroblast proliferation, migration, contraction, and extracellular matrix (ECM) production. It sustains mitochondrial redox balance by stabilizing the antioxidant protein PRDX3, thereby reducing ROS‐induced damage. In diabetic foot ulcers, hypoxia, hyperglycemia, and reduced FGF2 and EGF expression lower
Guanglin Niu +13 more
wiley +1 more source
Phospholipid removal during degradation of rat plasma very low density lipoprotein in vitro1
Journal of Lipid Research, 1976 The hydrolysis of glycerophospholipids in very low density lipoprotein by enzyme(s) released into circulation after the injection of heparin to rats was studied.
S Eisenberg, D Schurr
doaj +1 more source
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring [PDF]
, 2002 Surface proteins of Staphylococcus aureus are anchored to the cell wall peptidoglycan by a mechanism requiring a C-terminal sorting signal with an LPXTG motif.
Mazmanian, Sarkis K. +3 more
core