Results 221 to 230 of about 609,890 (261)
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2020
In this chapter, we go over the main principles and protocols that enable to keep cells and tissues alive in an in vitro environment. This process is called “cell culturing” and can be used for both isolated cells and small tissue constructs. After touching upon main terms such as primary, secondary, continuous, and finite cell lines, we focus on ...
Astghik Karapetyan, Narine Sarvazyan
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In this chapter, we go over the main principles and protocols that enable to keep cells and tissues alive in an in vitro environment. This process is called “cell culturing” and can be used for both isolated cells and small tissue constructs. After touching upon main terms such as primary, secondary, continuous, and finite cell lines, we focus on ...
Astghik Karapetyan, Narine Sarvazyan
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Esophageal stem cells and 3D‐cell culture models
Annals of the New York Academy of Sciences, 2011The following on esophageal stem cells and 3D‐cell culture models contains commentaries on metaplasia through transdifferentiation and through stem cells; transcription factors that may determine an intestinal‐like phenotype; the in vitro, organotypic cell culture models; and the role of stem cells in Barrett's esophagus and its dysplastic progression.
Rhonda F, Souza +2 more
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2017
In the 1960s, scientists searching for a cell-based system that would reflect the physiology and heterogeneity of solid tumors in a well-controlled experimental setting developed multicell spheroid cultures. Early studies focused on the biology, physiology, and drug and radiation response of spheroids.
David M. Evans, Beverly A. Teicher
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In the 1960s, scientists searching for a cell-based system that would reflect the physiology and heterogeneity of solid tumors in a well-controlled experimental setting developed multicell spheroid cultures. Early studies focused on the biology, physiology, and drug and radiation response of spheroids.
David M. Evans, Beverly A. Teicher
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Injectable thermogel for 3D culture of stem cells
Biomaterials, 2018Thermogel is an aqueous polymer solution that undergoes sol-to-gel transition as the temperature increases. Cells, growth factors, and signaling molecules can be incorporated simultaneously during the sol-to-gel transition. The cytocompatible procedure makes the thermogel an excellent platform for 3D culture of stem cells.
Madhumita, Patel +4 more
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3D Cell Culture Systems: Advantages and Applications
Journal of Cellular Physiology, 2014Cell cultures are important material of study for the variety of advantages that they offer. Both established continuous cell lines and primary cell cultures continue to be invaluable for basic research and for direct applications. Technological advancements are necessary to address emerging complex challenges and the way cells are cultured in vitro is
Maddaly, Ravi +4 more
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Nanoimprinting of Topographical and 3D Cell Culture Scaffolds
Nanomedicine, 2014The extracellular matrix exhibits several nanostructures such as fibers, filaments, nanopores and ridges that can be mimicked by topographical and 3D substrates for cell and tissue cultures for an environment closer to in vivo conditions. This review summarizes and discusses a growing number of reports employing nanoimprint lithography to obtain such ...
Maha, Elsayed, Olivia M, Merkel
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3D-FISH on Cultured Cells Combined with Immunostaining
2010Fluorescence in situ hybridization on three-dimensionally preserved nuclei (3D-FISH), in combination with immunocytochemistry and 3D fluorescence microscopy, is a key tool to analyze the functional organization of the interphase nucleus. In the last decade, 3D-FISH on cultured cells has become a routine technique and is now widely used in nuclear ...
Irina, Solovei, Marion, Cremer
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Fabrication of Cell Spheroids for 3D Cell Culture and Biomedical Applications
Biochip Journal, 2022Hye Jin Hong, Hyun Jong Lee
exaly