Results 221 to 230 of about 155,151 (257)

The bacterial 4S pathway – an economical alternative for crude oil desulphurization that reduces CO2 emissions

Green Chemistry, 2020
We discuss structural and mechanistic aspects of the Dsz enzymes in the 4S pathway, with a focus on rational molecular strategies for enzyme engineering, aiming at enzyme catalytic rate and efficiency improvement to meet industrial demands.
João P. M. Sousa, Pedro Ferreira, Rui P. P. Neves, Maria J. Ramos, Pedro A. Fernandes   +4 more
openaire   +1 more source

Metabolic kinetic model for dibenzothiophene desulfurization through 4S pathway using intracellular compound concentrations

Biochemical Engineering Journal, 2017
Abstract Dibenzothiophene desulfurization with Pseudomonas putida CECT5279 in resting cells has been studied. A rational evolution of the kinetic modeling of the 4S desulfurization route is presented based on the recent advances in the knowledge of this bioprocess.
Igor Martínez, Victoria E. Santos, Fèlix Garcìa-Ochoa   +2 more
openaire   +1 more source

Elucidation of a [4Fe-4S] cluster degradation pathway: rapid kinetic studies of the degradation of Chromatium vinosum HiPIP

JBIC Journal of Biological Inorganic Chemistry, 2001
Irreversible disassembly of the 4Fe-4S cluster in Chromatium vinosum high-potential iron protein (HiPIP) has been investigated in the presence of a low concentration of guanidinium hydrochloride. From the dependence of degradation rate on [H+], it is deduced that at least three protons are required to trigger efficient cluster degradation.
M W, Foster, S, Bian, K K, Surerus, J A, Cowan   +3 more
openaire   +2 more sources

Contribution of the Kodama and 4S pathways to the dibenzothiophene biodegradation in different coastal wetlands under different C/N ratios

Journal of Environmental Sciences, 2019
Dibenzothiophene (DBT) degradation mechanisms and the transformation of pathways during the incubation of three types of coastal sediments with C/N ratios ranging from 1 to 9 were investigated. The DBT degradation efficiencies were clearly improved with increasing C/N ratio in reed wetland sediments, tidal wetlands sediments and estuary wetland ...
Lei Wang, Guodong Ji, Siqiao Huang
openaire   +2 more sources

The Effect of Elimination of thedszCGene in Energy Consuming of Biodesulfurization in Broken 4S Pathway

Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 2011
Abstract In this study, the dszC gene was eliminated in the 4S pathway to evaluate the energy consuming in the biodesulfurization process. Therefore, a new recombinant plasmid was constructed harboring the dszAB gene of Rhodococcus erythropolis IGTS8 was transferred into E. coli DH5α. In addition, another construct harboring the complete dsz operon was
J. Raheb, M. J. Hajipour
openaire   +1 more source

Enhancement/evolution of biodesulfurization 4S pathway by genetic engineering and bioinformatic approaches

2019
Biodesulfurization could be a beneficial method in the industry for lowering the sulfur content of crude oil and could improve the efficiency of currently and costly used method, i.e., hydrodesulfurization. But, to achieve this goal, it is critical to enhancing the BDS rate using genetics and other omics approaches.
Borhani, Matia Sadat, Etemadifar, Zahra
openaire   +1 more source

A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo

Biotechnology Letters, 2019
To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes.This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be A:B:C = 1:2:4 and 1:4:2.
Lu Li, Lei Ye, Zhijie Guo, Wei Zhang, Xihao Liao, Ying Lin, Shuli Liang   +6 more
openaire   +2 more sources

Isoprenoid Biosynthesis through the Methylerythritol Phosphate Pathway: The (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate Synthase (GcpE) is a [4Fe–4S] Protein

Angewandte Chemie International Edition, 2002
nitrilotriaceticacidagarosecolumn. The enzyme was found to be 95% pure by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electro-phoresis) and presented an apparent molecular mass of43kDa.
Myriam, Seemann, Bernadette Tse Sum, Bui, Murielle, Wolff, Denis, Tritsch, Narciso, Campos, Albert, Boronat, Andrée, Marquet, Michel, Rohmer   +7 more
openaire   +2 more sources

Study of a newly isolated thermophilic bacterium capable of Kuhemond heavy crude oil and dibenzothiophene biodesulfurization following 4S pathway at 60 °C

Journal of Chemical Technology & Biotechnology, 2008
AbstractBACKGROUND: To meet stringent emission standards stipulated by regulatory agencies, the oil industry is required to bring down the sulfur content in fuels. As some compounds cannot be desulfurized by existing desulfurizing processes (such as hydrodesulfurization, HDS) biodesulfurization has become an interesting topic for researchers.
Sarah Torkamani, Jalal Shayegan, Soheila Yaghmaei, Iran Alemzadeh   +3 more
openaire   +1 more source

Isoprenoid Biosynthesis via the MEP Pathway: In Vivo Mössbauer Spectroscopy Identifies a [4Fe-4S] ²⁺ Center with Unusual Coordination Sphere in the LytB Protein

Journal of the American Chemical Society, 2009
The MEP pathway for the biosynthesis of isoprene units is present in most pathogenic bacteria, in the parasite responsible for malaria, and in plant plastids. This pathway is absent in animals and is accordingly a target for the development of antimicrobial drugs. LytB, also called IspH, the last enzyme of this pathway catalyzes the conversion of (E)-4-
Myriam, Seemann, Karnjapan, Janthawornpong, Julia, Schweizer, Lars H, Böttger, Adam, Janoschka, Anne, Ahrens-Botzong, Erasmienne Ngouamegne, Tambou, Olaf, Rotthaus, Alfred X, Trautwein, Michel, Rohmer   +9 more
openaire   +2 more sources