Results 1 to 10 of about 1,602 (228)
Macromolecular Ab Initio Phasing Enforcing Secondary and Tertiary Structure [PDF]
IUCrJ, 2015 Ab initio phasing of macromolecular structures, from the native intensities alone with no experimental phase information or previous particular structural knowledge, has been the object of a long quest, limited by two main barriers: structure size and ...
Massimo Sammito +2 more
exaly +4 more sources
High-throughput automated molecular replacement for small-molecule MicroED data [PDF]
IUCrJInterest in electron diffraction (ED) for structural characterization of both proteins and small molecules has grown significantly over the last decade.
Adam Thibodeaux +3 more
doaj +4 more sources
Ab-initio phasing using nanocrystal shape transforms with incomplete unit cells [PDF]
IUCrJ, 2014 X-ray free electron lasers are used in measuring diffraction patterns from nanocrystals in the `diffract-before-destroy' mode by outrunning radiation damage. The finite-sized nanocrystals provide an opportunity to recover intensity between Bragg spots by
Haiguang Liu +2 more
doaj +2 more sources
Direct from the seed: an atomic resolution protein structure by ab initio MicroED [PDF]
Nature CommunicationsWhile purifying the seed protein crambin, we find that needles of pure protein nanocrystals form spontaneously during the drying of a simple ethanolic purification drop.
Purna Chandra Rao Vasireddy +5 more
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Resolution Dependence of an Ab Initio Phasing Method in Protein X-Ray Crystallography
Crystals, 2018 For direct phasing of protein crystals, a method based on the hybrid-input-output (HIO) algorithm has been proposed and tested on a variety of structures.
Hongxing He
exaly +3 more sources
Direct Phasing of Coiled-Coil Protein Crystals
Crystals, 2022 Coiled-coil proteins consisting of multiple copies of helices take part in transmembrane transportation and oligomerization, and are used for drug delivery.
Ruijiang Fu, Wu-Pei Su, Hongxing He
doaj +1 more source
Extending Ab Initio Phasing up to 2.2 Å Resolution: New Superposition Techniques
Crystals, 2023 Patterson superposition techniques are a historical method for solving the structures of small molecules ab initio, provided they contain heavy atoms in the unit cell.
Maria Cristina Burla +4 more
doaj +1 more source
Computational and Structural Biotechnology Journal, 2022 Computational methods for protein structure prediction have made significant strides forward, as evidenced by the last development of the neural network AlphaFold, which outperformed the CASP14 competitors by consistently predicting the structure of ...
Eugenio Ferrario +3 more
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Routine phasing of coiled-coil protein crystal structures with AMPLE
IUCrJ, 2015 Coiled-coil protein folds are among the most abundant in nature. These folds consist of long wound α-helices and are architecturally simple, but paradoxically their crystallographic structures are notoriously difficult to solve with molecular-replacement
Jens M. H. Thomas +5 more
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Draft crystal structure of the vault shell at 9-A resolution. [PDF]
PLoS Biology, 2007 Vaults are the largest known cytoplasmic ribonucleoprotein structures and may function in innate immunity. The vault shell self-assembles from 96 copies of major vault protein and encapsulates two other proteins and a small RNA. We crystallized rat liver
Daniel H Anderson +4 more
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