Transformation of Acetobacter xylinum with plasmid DNA by electroporation
Plasmid, 1992Genetic analysis of Acetobacter xylinum, a cellulose-synthesizing bacterium, has been limited by lack of a successful transformation method. Transformation of A. xylinum was attempted using two broad-host-range plasmids (pUCD2 and pRK248) and a variety of transformation methods.
P E, Hall +3 more
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Synthesis of Cellulose by Resting Cells of Acetobacter xylinum
Nature, 1947IN plants, cellulose is formed intracellularly, by a process the chemistry of which is completely unknown. Microscopic evidence adduced by Farr suggests that the conditions of cellulose formation in green plants are very complex1,2. But in cultures of the bacterium Acetobacter xylinum, true cellulose3,4,5,6 in the form of a mesh of beautifully defined ...
S, HESTRIN, M, ASCHNER, J, MAGER
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THE FORMATION OF CELLULOSE MICROFIBRILS IN SUSPENSIONS OF ACETOBACTER XYLINUM
Canadian Journal of Microbiology, 1960Formation of cellulose microfibrils from precursors elaborated by Acetobacter xylinum takes place remote from the cell surface and without an extensive, amorphous, intermediate high polymer. The microfibrils increase in mass by growth only at one or both tips. The rate of microfibrillar growth per bacterial cell at 25 °C is constant at 0.1 μ per minute
J R, COLVIN, M, BEER
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Phosphorylation coupled to malate oxidation in Acetobacter xylinum
Biochemical and Biophysical Research Communications, 1966Abstract The oxidation of malate to oxaloacetate in A. xylinum has been shown to be irreversible. It is catalyzed by a FAD flavoprotein which is not affected by high concentrations of oxaloacetate and is linked to the cytochrome chain by a vitamin K-like compound ( Benziman and Abeliovitz, 1964 ; Benziman and Galenter, 1964 ; Benziman and ...
M, Benziman, L, Levy
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Helical, non-cellulosic microfibrils from acetobacter xylinum and acetobacter suboxydans
Biochimica et Biophysica Acta, 1962Abstract Helical, non-cellulosic microfibrils are formed in water from substance(s) soluble in 80% ethanol, which are concentrated in (or close to) the cell walls of Acetobacter xylinum and Acetobacter suboxydans but not in those of Acetobacter acetigenum or Acetobacter aceti .
A L, CURRIE, N, RAMANATHAN, J R, COLVIN
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Synthesis of mannosyl cellobiose diphosphate prenol in Acetobacter xylinum
Archives of Biochemistry and Biophysics, 1980Abstract The enzymatic synthesis of a β-mannosyl (1 → 3) β-glucosyl (1 → 4) α-glucose-1-pyrophosphate-prenol (allylic) by Acetobacter xylinum preparations is described. Glucose pyrophosphate lipid, already known to be formed from UDP-glucose and endogenous phosphate lipid, is demonstrated to accept another glucose from UDP-glucose to give a ...
R O, Couso +3 more
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THE FORMATION OF CELLULOSE MICROFIBRILS BY ACETOBACTER XYLINUM IN AGAR SURFACES
Canadian Journal of Microbiology, 1961The formation of extracellular cellulose microfibrils by Acetobacter xylinum on agar surfaces is remote from the cell membrane and does not involve an intermediate, amorphous high polymer, in agreement with conclusions from studies of liquid suspensions.
B, MILLMAN, J R, COLVIN
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Synthesis of Cellulose in Ethanol Extracts of Acetobacter xylinum
Nature, 1959FORMATION of bacterial cellulose in cell-free fractions of homogenates of Acetobacter xylinum, supplemented by either adenosine triphosphate1,2 or uridine diphosphoglucose3, has recently been reported. Such homogenate fractions are necessarily complex, and unequivocal identification of the immediate precursor of cellulose might be easier if a cell-free
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Structural studies of acetan, an exopolysaccharide elaborated by Acetobacter xylinum
Carbohydrate Research, 1993The exopolysaccharide acetan, elaborated by Acetobacter xylinum, has been investigated. The polysaccharide and a heptasaccharide, obtained on enzymic hydrolysis, corresponding to the repeating unit were characterised by sugar and methylation analysis and by NMR spectroscopy and MS.
P E, Jansson +3 more
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Regulation of endoglucanase gene (cmcax) expression in Acetobacter xylinum
Journal of Bioscience and Bioengineering, 2008Although cellulose is the most abundant biopolymer in nature, the detailed mechanisms of cellulose biosynthesis remain unknown. Acetobacter xylinum is one of the best-studied model organisms for cellulose biosynthesis. Interestingly, the over-expression of the cmcax gene cause enhancement of cellulose production in A. xylinum, while its product (CMCax)
Shin, Kawano +6 more
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