Effects of Non-Ionic Micelles on the Acid-Base Equilibria of a Weak Polyelectrolyte. [PDF]
Yekymov E +4 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
This study examines the impact of a Science–Technology–Society–Environment (STSE) educational intervention on the teaching of acid–base reactions to 11th-grade students (n = 17).
Gonçalo Gorito, Carla Morais
doaj +1 more source
Cerebrospinal fluid and arterial acid-base equilibria in spontaneously breathing third-trimester pregnant women. [PDF]
Zadek F +10 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Interfacial Acid-Base Equilibria and Electric Fields Concurrently Probed by <i>In Situ</i> Surface-Enhanced Infrared Spectroscopy. [PDF]
Delley MF, Nichols EM, Mayer JM.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Spectral, cyclic voltammetric and molecular modeling studies have been carried out on some 3-(2-hydroxybenzalhydrazino-4-thiozolyl)coumarins (1), to understand their solution and electrochemical characteristics.
N. Raju, A. Ramachandraiah
doaj +1 more source
The Importance of Acid-Base Equilibria in Bicarbonate Electrolytes for CO2 Electrochemical Reduction and CO Reoxidation Studied on Au(hkl) Electrodes. [PDF]
Marcandalli G, Villalba M, Koper MTM.
europepmc +1 more source
A Depolarizing Leak in Sodium Bicarbonate Cotransporter NBCe1 Causes Brain Edema
ABSTRACT Objectives SLC4A4 encodes electrogenic sodium bicarbonate cotransporter NBCe1, prominently expressed in kidney and brain. Recessive loss‐of‐function variants in SLC4A4 cause proximal renal tubular acidosis, no brain edema. In the brain, NBCe1 is expressed by astrocytes, where it regulates pH and mediates astrocyte volume changes.
Quinty Bisseling +16 more
wiley +1 more source

