Results 81 to 90 of about 59,806 (250)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

STUDY OF ELECTROCHEMICAL BEHAVIOR OF VI-B GROUP METAL OXIDES IN TUNGSTATE MELT

Studia Universitatis Babes-Bolyai Chemia
Thermodynamic assessment of the probability of interaction of metal oxides of group VI-B with tungstate melts, the results of the study of the acid-base properties of tungstate melt by potentiometric method.
Victor MALYSHEV, Angelina GAB, Ana-Maria POPESCU, Virgil CONSTANTIN   +3 more
doaj   +1 more source

An Exact Method to Determine the Conductivity of Aqueous Solutions in Acid-Base Titrations

Journal of Chemistry, 2015
Several works in the literature show that it is possible to establish the analytic equations to estimate the volume V of a strong base or a strong acid (Vb and Va, resp.) being added to a solution of a substance or a mix of substances during an acid-base
Norma Rodríguez-Laguna, Alberto Rojas-Hernández, María Teresa Ramírez-Silva, Lucero Hernández-García, Mario Romero-Romo   +4 more
doaj   +1 more source

Electrochemical oxidation of oxalic acid and hydrazinium nitrate on platinum in nitric acid media [PDF]

, 2012
Several studies in the literature have investigated the electrochemical effects of oxalic acid and hydrazine on various materials in neutral (pH buffered to 7), basic or weakly acidic media (pH 6).
Féraud, Jean-Pierre, Ode, Denis, Queffelec, Benoit, Rockombeny, L.C., Tzedakis, Théodore   +4 more
core   +2 more sources

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Organisation of the Analytical, Stoichiometric, and Thermodynamic Information for water Chemistry Calculations [PDF]

, 2015
A common feature of the chemical processes of the hydrosphere and water treatment plants is that essentially the same types of chemical equilibrium reactions occur in both fields.
Földényi, Rita, Marton, Aurél
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Study of β-Cyclodextrin Effect on Acidity Constant of 2-(2H-Benzo(d)(1,2,3) triazol-2-yl) phenol by Spectrophotometry

E-Journal of Chemistry, 2010
The interaction of solutes with β-cyclodextrin leads to apparent changes in their chemical properties, such as shifts in their absorption spectra and acid-base equilibrium.
Amir H. M. Sarrafi, Elahe Konoz, Negin Ghorashi   +2 more
doaj   +1 more source

Acidification of musts in warm regions with tartaric acid and calcium sulfate at industrial scale [PDF]

, 2015
Acidification of musts is necessary in warm areas where high temperatures during ripening accelerate breathing combustion of tartaric acid and, in particular, malic acid in the berries.
Cubillana Aguilera, Laura, Gómez Benítez, Juan, Lasanta Melero, Cristina, Palacios Santander, José María   +3 more
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source