Results 101 to 110 of about 3,329,624 (262)
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Acid phosphatase activity in human semen
, 1988 Acid phosphatase activity was examined in normal and abnormal human semen of individuals not suffering from testosterone deficiency. No differences in enzymatic activity between the examined groups were observed.
Miriam Sagiv, D. +10 more
core +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Frontiers in Plant Science, 2017 Phytate is the most abundant form of soil organic phosphorus (P). Increased P nutrition of arbuscular mycorrhizal plants derived from phytate has been repeatedly reported.
Gu Feng +4 more
doaj +1 more source
Ciencias Marinas, 2006 A subchronic toxicity study was performed by exposing organisms to two surfactants in a continuous flow-through system for 120 h, followed by a depuration phase of 48 h.
D Álvarez-Muñoz +4 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
ASSOCIATION OF ACID AND ALKALINE PHOSPHATASE AMONG THE THYROID PATIENTS [PDF]
, 2013 Thyroidism is becoming one of the most prevalent diseases due to the malfunctioning of the thyroid gland. The objective of the present study is to observe the association of acid phosphatase and alkaline phosphatase with thyroid disorder.
PRATHYUSHA, U., B.DHARANI PRIYA
core +1 more source
Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
Bio-Protocol, 2019 Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells.
Amalia Papadaki, Haralabia Boleti
doaj +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Threonine 348 regulates the subcellular localization of PTEN
FEBS Open Bio, EarlyView.Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source