Results 1 to 10 of about 2,356 (197)

Targeting Transcriptional Regulators Affecting Acarbose Biosynthesis in Actinoplanes sp. SE50/110 Using CRISPRi Silencing [PDF]

Microorganisms
Acarbose, a pseudo-tetrasaccharide produced by Actinoplanes sp. SE50/110, is an α-glucosidase inhibitor and is used as a medication to treat type 2 diabetes.
Saskia Dymek, Lucas Jacob, Alfred Pühler, Jörn Kalinowski   +3 more
doaj   +3 more sources

Discovery of Thiazostatin D/E Using UPLC-HR-MS2-Based Metabolomics and σ-Factor Engineering of Actinoplanes sp. SE50/110 [PDF]

Frontiers in Bioengineering and Biotechnology
As the natural producer of acarbose, Actinoplanes sp. SE50/110 has high industrial relevance. Like most Actinobacteria, the strain carries several more putative biosynthetic gene clusters (BGCs) to produce further natural products, which are to be ...
Johan Isaksson, Jeanette H. Andersen, Kine Østnes Hansen   +2 more
exaly   +4 more sources

Involvement of a Putative Acyltransferase Gene in Sporangium Formation in Actinoplanes missouriensis. [PDF]

Microbiology Spectrum
The actinomycete Actinoplanes missouriensis forms branched substrate mycelia during vegetative growth and produces terminal sporangia, each of which contains a few hundred spherical flagellated spores, from the substrate mycelia through short ...
Yasuo Ohnishi, Takeaki Tezuka, Shixuan Hu   +2 more
exaly   +4 more sources

Involvement of an orphan response regulator of the two-component regulatory system in the formation of physiologically mature sporangia in Actinoplanes missouriensis [PDF]

Microbiology Spectrum
The actinomycete Actinoplanes missouriensis forms terminal sporangia that contain dormant sporangiospores. Upon contact with water, sporangia release zoospores through a process called sporangium dehiscence.
Takuya Akutsu, Zhuwen Tan, Aiko Hirata, Takeaki Tezuka, Yasuo Ohnishi   +4 more
doaj   +2 more sources

Components of the Clp complex are required for sporangium formation and dehiscence in Actinoplanes missouriensis [PDF]

Journal of Bacteriology
The actinomycete Actinoplanes missouriensis forms terminal sporangia that contain a few hundred spores. When immersed in water, the sporangium opens to release spores, which start swimming using flagella, via a process referred to as sporangium ...
Ryota Suzuki, Manato Maruko, Remi Suzuki, Takeaki Tezuka, Yasuo Ohnishi   +4 more
doaj   +2 more sources

Genomic insights into Dactylosporangium sp. AC04546, a rubber degrader with three latex clearing proteins [PDF]

Frontiers in Microbiology
With more than 100 rubber-degrading strains being reported, only 9 Lcp proteins isolated from Nocardia, Gordonia, Streptomyces, Rhodococcus, Actinoplanes, and Solimonas have been purified and biochemically characterized.
Ann Anni Basik, Namiko Gibu, Yukimura Kawagiwa, Siuk-Mun Ng, Tiong Chia Yeo, Kumar Sudesh, Daisuke Kasai   +6 more
doaj   +2 more sources

Class E sortase SrtE and two SrtE-dependent cell wall-anchored hydrophobic proteins are involved in morphogenesis in Actinoplanes missouriensis: occurrence of exploratory growth beyond genus Streptomyces [PDF]

mBio
The actinomycete Actinoplanes missouriensis grows by extending branched hyphae and forms terminal sporangia containing a few hundred spores. A sortase-dependent spore surface protein, SspA, has recently been shown to be important for sporangium ...
Zhuwen Tan, Kazuki Nosho, Kyohei Umebayashi, Kenji Akamatsu, Reiichi Ariizumi, Takeaki Tezuka, Yasuo Ohnishi   +6 more
doaj   +2 more sources

Two glycoside hydrolases decompose the sporangium matrix to release spores during sporangium dehiscence in Actinoplanes missouriensis [PDF]

mBio
The actinomycete Actinoplanes missouriensis grows as a substrate mycelium and forms terminal sporangia containing a few hundred spores surrounded by an unidentified matrix called the sporangium matrix.
Kyota Mitsuyama, Shixuan Hu, Naoki Sunagawa, Kiyohiko Igarashi, Takeaki Tezuka, Yasuo Ohnishi   +5 more
doaj   +2 more sources

Sigma Factor Engineering in Actinoplanes sp. SE50/110: Expression of the Alternative Sigma Factor Gene ACSP50_0507 (σH^As) Enhances Acarbose Yield and Alters Cell Morphology [PDF]

Microorganisms
Sigma factors are transcriptional regulators that are part of complex regulatory networks for major cellular processes, as well as for growth phase-dependent regulation and stress response. Actinoplanes sp.
Laura Schlüter, Tobias Busche, Laila Bondzio, Andreas Hütten, Karsten Niehaus, Susanne Schneiker-Bekel, Alfred Pühler, Jörn Kalinowski   +7 more
doaj   +2 more sources

Role of MalQ Enzyme in a Reconstructed Maltose/Maltodextrin Pathway in Actinoplanes sp. SE50/110 [PDF]

Microorganisms
The pseudotetrasaccharide acarbose, produced by Actinoplanes sp. SE50/110, is a relevant secondary metabolite used in diabetes type II medication.
Camilla März, Sophia Nölting, Lars Wollenschläger, Alfred Pühler, Jörn Kalinowski   +4 more
doaj   +2 more sources